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Retroviral vector insertion sites associated with dominant hematopoietic clones mark "stemness" pathways.

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Retroviral vector insertion sites associated with dominant hematopoietic clones mark "stemness" pathways. / Kustikova, Olga S; Geiger, Hartmut; Li, Zhixiong; Brugman, Martijn H; Chambers, Stuart M; Shaw, Chad A; Pike-Overzet, Karin; de Ridder, Dick; Staal, Frank J T; von Keudell, Gottfried; Cornils, Kerstin; Nattamai, Kalpana Jekumar; Modlich, Ute; Wagemaker, Gerard; Goodell, Margaret A; Fehse, Boris; Baum, Christopher.

In: BLOOD, Vol. 109, No. 5, 5, 2007, p. 1897-1907.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kustikova, OS, Geiger, H, Li, Z, Brugman, MH, Chambers, SM, Shaw, CA, Pike-Overzet, K, de Ridder, D, Staal, FJT, von Keudell, G, Cornils, K, Nattamai, KJ, Modlich, U, Wagemaker, G, Goodell, MA, Fehse, B & Baum, C 2007, 'Retroviral vector insertion sites associated with dominant hematopoietic clones mark "stemness" pathways.', BLOOD, vol. 109, no. 5, 5, pp. 1897-1907. <http://www.ncbi.nlm.nih.gov/pubmed/17119121?dopt=Citation>

APA

Kustikova, O. S., Geiger, H., Li, Z., Brugman, M. H., Chambers, S. M., Shaw, C. A., Pike-Overzet, K., de Ridder, D., Staal, F. J. T., von Keudell, G., Cornils, K., Nattamai, K. J., Modlich, U., Wagemaker, G., Goodell, M. A., Fehse, B., & Baum, C. (2007). Retroviral vector insertion sites associated with dominant hematopoietic clones mark "stemness" pathways. BLOOD, 109(5), 1897-1907. [5]. http://www.ncbi.nlm.nih.gov/pubmed/17119121?dopt=Citation

Vancouver

Kustikova OS, Geiger H, Li Z, Brugman MH, Chambers SM, Shaw CA et al. Retroviral vector insertion sites associated with dominant hematopoietic clones mark "stemness" pathways. BLOOD. 2007;109(5):1897-1907. 5.

Bibtex

@article{d7a72b20acdd4f6392da4b31448bd22d,
title = "Retroviral vector insertion sites associated with dominant hematopoietic clones mark {"}stemness{"} pathways.",
abstract = "Evidence from model organisms and clinical trials reveals that the random insertion of retrovirus-based vectors in the genome of long-term repopulating hematopoietic cells may increase self-renewal or initiate malignant transformation. Clonal dominance of nonmalignant cells is a particularly interesting phenotype as it may be caused by the dysregulation of genes that affect self-renewal and competitive fitness. We have accumulated 280 retrovirus vector insertion sites (RVISs) from murine long-term studies resulting in benign or malignant clonal dominance. RVISs (22.5%) are located in or near (up to 100 kb [kilobase]) to known proto-oncogenes, 49.6% in signaling genes, and 27.9% in other or unknown genes. The resulting insertional dominance database (IDDb) shows substantial overlaps with the transcriptome of hematopoietic stem/progenitor cells and the retrovirus-tagged cancer gene database (RTCGD). RVISs preferentially marked genes with high expression in hematopoietic stem/progenitor cells, and Gene Ontology revealed an overrepresentation of genes associated with cell-cycle control, apoptosis signaling, and transcriptional regulation, including major {"}stemness{"} pathways. The IDDb forms a powerful resource for the identification of genes that stimulate or transform hematopoietic stem/progenitor cells and is an important reference for vector biosafety studies in human gene therapy.",
author = "Kustikova, {Olga S} and Hartmut Geiger and Zhixiong Li and Brugman, {Martijn H} and Chambers, {Stuart M} and Shaw, {Chad A} and Karin Pike-Overzet and {de Ridder}, Dick and Staal, {Frank J T} and {von Keudell}, Gottfried and Kerstin Cornils and Nattamai, {Kalpana Jekumar} and Ute Modlich and Gerard Wagemaker and Goodell, {Margaret A} and Boris Fehse and Christopher Baum",
year = "2007",
language = "Deutsch",
volume = "109",
pages = "1897--1907",
journal = "BLOOD",
issn = "0006-4971",
publisher = "American Society of Hematology",
number = "5",

}

RIS

TY - JOUR

T1 - Retroviral vector insertion sites associated with dominant hematopoietic clones mark "stemness" pathways.

AU - Kustikova, Olga S

AU - Geiger, Hartmut

AU - Li, Zhixiong

AU - Brugman, Martijn H

AU - Chambers, Stuart M

AU - Shaw, Chad A

AU - Pike-Overzet, Karin

AU - de Ridder, Dick

AU - Staal, Frank J T

AU - von Keudell, Gottfried

AU - Cornils, Kerstin

AU - Nattamai, Kalpana Jekumar

AU - Modlich, Ute

AU - Wagemaker, Gerard

AU - Goodell, Margaret A

AU - Fehse, Boris

AU - Baum, Christopher

PY - 2007

Y1 - 2007

N2 - Evidence from model organisms and clinical trials reveals that the random insertion of retrovirus-based vectors in the genome of long-term repopulating hematopoietic cells may increase self-renewal or initiate malignant transformation. Clonal dominance of nonmalignant cells is a particularly interesting phenotype as it may be caused by the dysregulation of genes that affect self-renewal and competitive fitness. We have accumulated 280 retrovirus vector insertion sites (RVISs) from murine long-term studies resulting in benign or malignant clonal dominance. RVISs (22.5%) are located in or near (up to 100 kb [kilobase]) to known proto-oncogenes, 49.6% in signaling genes, and 27.9% in other or unknown genes. The resulting insertional dominance database (IDDb) shows substantial overlaps with the transcriptome of hematopoietic stem/progenitor cells and the retrovirus-tagged cancer gene database (RTCGD). RVISs preferentially marked genes with high expression in hematopoietic stem/progenitor cells, and Gene Ontology revealed an overrepresentation of genes associated with cell-cycle control, apoptosis signaling, and transcriptional regulation, including major "stemness" pathways. The IDDb forms a powerful resource for the identification of genes that stimulate or transform hematopoietic stem/progenitor cells and is an important reference for vector biosafety studies in human gene therapy.

AB - Evidence from model organisms and clinical trials reveals that the random insertion of retrovirus-based vectors in the genome of long-term repopulating hematopoietic cells may increase self-renewal or initiate malignant transformation. Clonal dominance of nonmalignant cells is a particularly interesting phenotype as it may be caused by the dysregulation of genes that affect self-renewal and competitive fitness. We have accumulated 280 retrovirus vector insertion sites (RVISs) from murine long-term studies resulting in benign or malignant clonal dominance. RVISs (22.5%) are located in or near (up to 100 kb [kilobase]) to known proto-oncogenes, 49.6% in signaling genes, and 27.9% in other or unknown genes. The resulting insertional dominance database (IDDb) shows substantial overlaps with the transcriptome of hematopoietic stem/progenitor cells and the retrovirus-tagged cancer gene database (RTCGD). RVISs preferentially marked genes with high expression in hematopoietic stem/progenitor cells, and Gene Ontology revealed an overrepresentation of genes associated with cell-cycle control, apoptosis signaling, and transcriptional regulation, including major "stemness" pathways. The IDDb forms a powerful resource for the identification of genes that stimulate or transform hematopoietic stem/progenitor cells and is an important reference for vector biosafety studies in human gene therapy.

M3 - SCORING: Zeitschriftenaufsatz

VL - 109

SP - 1897

EP - 1907

JO - BLOOD

JF - BLOOD

SN - 0006-4971

IS - 5

M1 - 5

ER -