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Retinoids induce differential expression and DNA binding of the mouse germ cell nuclear factor in P19 embryonal carcinoma cells

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Retinoids induce differential expression and DNA binding of the mouse germ cell nuclear factor in P19 embryonal carcinoma cells. / Heinzer, C; Süsens, U; Schmitz, T P; Borgmeyer, U.

In: BIOL CHEM, Vol. 379, No. 3, 03.1998, p. 349-59.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Heinzer, C, Süsens, U, Schmitz, TP & Borgmeyer, U 1998, 'Retinoids induce differential expression and DNA binding of the mouse germ cell nuclear factor in P19 embryonal carcinoma cells', BIOL CHEM, vol. 379, no. 3, pp. 349-59.

APA

Heinzer, C., Süsens, U., Schmitz, T. P., & Borgmeyer, U. (1998). Retinoids induce differential expression and DNA binding of the mouse germ cell nuclear factor in P19 embryonal carcinoma cells. BIOL CHEM, 379(3), 349-59.

Vancouver

Heinzer C, Süsens U, Schmitz TP, Borgmeyer U. Retinoids induce differential expression and DNA binding of the mouse germ cell nuclear factor in P19 embryonal carcinoma cells. BIOL CHEM. 1998 Mar;379(3):349-59.

Bibtex

@article{9d64bf6d23ab4dcc93322159692372ca,
title = "Retinoids induce differential expression and DNA binding of the mouse germ cell nuclear factor in P19 embryonal carcinoma cells",
abstract = "The mouse germ cell nuclear factor (GCNF), a member of the nuclear receptor superfamily, is highly expressed during gametogenesis and in the developing nervous system. The in vitro translated protein binds as a homodimer to the direct repeat (DR) of the sequence -AGGTCA- (DR-0). In this report, we characterize a DR-0 binding activity in P19 cell extracts that is induced by retinoids. This induction is concentration dependent and specific for embryonal carcinoma cells. The cellular protein binds with the same specificity as in vitro expressed GCNF, but migrates as a slower complex, indicating interaction with partner proteins. Because antisera directed against GCNF recognize this complex, we propose that GCNF is part of the binding activity. Combining in vitro translated GCNF and extracts of non-expressing cells shows that such interactions can be formed posttranslationally. Northern analysis demonstrates a concentration dependent induction of GCNF mRNA by retinoic acid. A time course shows that the level of GCNF binding is transiently elevated, later downregulated, and not detectable in differentiated cells. We propose that GCNF regulation is an important step during determination of embryonal carcinoma cells.",
keywords = "Animals, Antibodies, Base Sequence, Binding, Competitive, Blotting, Northern, COS Cells, Carcinoma, Embryonal, DNA, DNA-Binding Proteins, Gene Expression Regulation, Neoplastic, Kinetics, Mice, Nuclear Receptor Subfamily 6, Group A, Member 1, Protein Binding, RNA, Messenger, Receptors, Cytoplasmic and Nuclear, Retinoids, Tumor Cells, Cultured",
author = "C Heinzer and U S{\"u}sens and Schmitz, {T P} and U Borgmeyer",
year = "1998",
month = mar,
language = "English",
volume = "379",
pages = "349--59",
journal = "BIOL CHEM",
issn = "1431-6730",
publisher = "Walter de Gruyter GmbH & Co. KG",
number = "3",

}

RIS

TY - JOUR

T1 - Retinoids induce differential expression and DNA binding of the mouse germ cell nuclear factor in P19 embryonal carcinoma cells

AU - Heinzer, C

AU - Süsens, U

AU - Schmitz, T P

AU - Borgmeyer, U

PY - 1998/3

Y1 - 1998/3

N2 - The mouse germ cell nuclear factor (GCNF), a member of the nuclear receptor superfamily, is highly expressed during gametogenesis and in the developing nervous system. The in vitro translated protein binds as a homodimer to the direct repeat (DR) of the sequence -AGGTCA- (DR-0). In this report, we characterize a DR-0 binding activity in P19 cell extracts that is induced by retinoids. This induction is concentration dependent and specific for embryonal carcinoma cells. The cellular protein binds with the same specificity as in vitro expressed GCNF, but migrates as a slower complex, indicating interaction with partner proteins. Because antisera directed against GCNF recognize this complex, we propose that GCNF is part of the binding activity. Combining in vitro translated GCNF and extracts of non-expressing cells shows that such interactions can be formed posttranslationally. Northern analysis demonstrates a concentration dependent induction of GCNF mRNA by retinoic acid. A time course shows that the level of GCNF binding is transiently elevated, later downregulated, and not detectable in differentiated cells. We propose that GCNF regulation is an important step during determination of embryonal carcinoma cells.

AB - The mouse germ cell nuclear factor (GCNF), a member of the nuclear receptor superfamily, is highly expressed during gametogenesis and in the developing nervous system. The in vitro translated protein binds as a homodimer to the direct repeat (DR) of the sequence -AGGTCA- (DR-0). In this report, we characterize a DR-0 binding activity in P19 cell extracts that is induced by retinoids. This induction is concentration dependent and specific for embryonal carcinoma cells. The cellular protein binds with the same specificity as in vitro expressed GCNF, but migrates as a slower complex, indicating interaction with partner proteins. Because antisera directed against GCNF recognize this complex, we propose that GCNF is part of the binding activity. Combining in vitro translated GCNF and extracts of non-expressing cells shows that such interactions can be formed posttranslationally. Northern analysis demonstrates a concentration dependent induction of GCNF mRNA by retinoic acid. A time course shows that the level of GCNF binding is transiently elevated, later downregulated, and not detectable in differentiated cells. We propose that GCNF regulation is an important step during determination of embryonal carcinoma cells.

KW - Animals

KW - Antibodies

KW - Base Sequence

KW - Binding, Competitive

KW - Blotting, Northern

KW - COS Cells

KW - Carcinoma, Embryonal

KW - DNA

KW - DNA-Binding Proteins

KW - Gene Expression Regulation, Neoplastic

KW - Kinetics

KW - Mice

KW - Nuclear Receptor Subfamily 6, Group A, Member 1

KW - Protein Binding

KW - RNA, Messenger

KW - Receptors, Cytoplasmic and Nuclear

KW - Retinoids

KW - Tumor Cells, Cultured

M3 - SCORING: Journal article

C2 - 9563832

VL - 379

SP - 349

EP - 359

JO - BIOL CHEM

JF - BIOL CHEM

SN - 1431-6730

IS - 3

ER -