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Retinal regions shape human and murine Müller cell proteome profile and functionality

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Retinal regions shape human and murine Müller cell proteome profile and functionality. / Kaplan, Lew; Drexler, Corinne; Pfaller, Anna M.; Brenna, Santra; Wunderlich, Kirsten A.; Dimitracopoulos, Andrea; Merl‐Pham, Juliane; Perez, Maria‐Theresa; Schlötzer‐Schrehardt, Ursula; Enzmann, Volker; Samardzija, Marijana; Puig, Berta; Fuchs, Peter; Franze, Kristian; Hauck, Stefanie M.; Grosche, Antje.

In: GLIA, Vol. 71, No. 2, 02.2023, p. 391-414.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kaplan, L, Drexler, C, Pfaller, AM, Brenna, S, Wunderlich, KA, Dimitracopoulos, A, Merl‐Pham, J, Perez, MT, Schlötzer‐Schrehardt, U, Enzmann, V, Samardzija, M, Puig, B, Fuchs, P, Franze, K, Hauck, SM & Grosche, A 2023, 'Retinal regions shape human and murine Müller cell proteome profile and functionality', GLIA, vol. 71, no. 2, pp. 391-414. https://doi.org/10.1002/glia.24283

APA

Kaplan, L., Drexler, C., Pfaller, A. M., Brenna, S., Wunderlich, K. A., Dimitracopoulos, A., Merl‐Pham, J., Perez, MT., Schlötzer‐Schrehardt, U., Enzmann, V., Samardzija, M., Puig, B., Fuchs, P., Franze, K., Hauck, S. M., & Grosche, A. (2023). Retinal regions shape human and murine Müller cell proteome profile and functionality. GLIA, 71(2), 391-414. https://doi.org/10.1002/glia.24283

Vancouver

Kaplan L, Drexler C, Pfaller AM, Brenna S, Wunderlich KA, Dimitracopoulos A et al. Retinal regions shape human and murine Müller cell proteome profile and functionality. GLIA. 2023 Feb;71(2):391-414. https://doi.org/10.1002/glia.24283

Bibtex

@article{726b9800eb4d4ae387d5761f46aeba40,
title = "Retinal regions shape human and murine M{\"u}ller cell proteome profile and functionality",
abstract = "The human macula is a highly specialized retinal region with pit-like morphology and rich in cones. How M{\"u}ller cells, the principal glial cell type in the retina, are adapted to this environment is still poorly understood. We compared proteomic data from cone- and rod-rich retinae from human and mice and identified different expression profiles of cone- and rod-associated M{\"u}ller cells that converged on pathways representing extracellular matrix and cell adhesion. In particular, epiplakin (EPPK1), which is thought to play a role in intermediate filament organization, was highly expressed in macular M{\"u}ller cells. Furthermore, EPPK1 knockout in a human M{\"u}ller cell-derived cell line led to a decrease in traction forces as well as to changes in cell size, shape, and filopodia characteristics. We here identified EPPK1 as a central molecular player in the region-specific architecture of the human retina, which likely enables specific functions under the immense mechanical loads in vivo.",
author = "Lew Kaplan and Corinne Drexler and Pfaller, {Anna M.} and Santra Brenna and Wunderlich, {Kirsten A.} and Andrea Dimitracopoulos and Juliane Merl‐Pham and Maria‐Theresa Perez and Ursula Schl{\"o}tzer‐Schrehardt and Volker Enzmann and Marijana Samardzija and Berta Puig and Peter Fuchs and Kristian Franze and Hauck, {Stefanie M.} and Antje Grosche",
year = "2023",
month = feb,
doi = "10.1002/glia.24283",
language = "English",
volume = "71",
pages = "391--414",
journal = "GLIA",
issn = "0894-1491",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Retinal regions shape human and murine Müller cell proteome profile and functionality

AU - Kaplan, Lew

AU - Drexler, Corinne

AU - Pfaller, Anna M.

AU - Brenna, Santra

AU - Wunderlich, Kirsten A.

AU - Dimitracopoulos, Andrea

AU - Merl‐Pham, Juliane

AU - Perez, Maria‐Theresa

AU - Schlötzer‐Schrehardt, Ursula

AU - Enzmann, Volker

AU - Samardzija, Marijana

AU - Puig, Berta

AU - Fuchs, Peter

AU - Franze, Kristian

AU - Hauck, Stefanie M.

AU - Grosche, Antje

PY - 2023/2

Y1 - 2023/2

N2 - The human macula is a highly specialized retinal region with pit-like morphology and rich in cones. How Müller cells, the principal glial cell type in the retina, are adapted to this environment is still poorly understood. We compared proteomic data from cone- and rod-rich retinae from human and mice and identified different expression profiles of cone- and rod-associated Müller cells that converged on pathways representing extracellular matrix and cell adhesion. In particular, epiplakin (EPPK1), which is thought to play a role in intermediate filament organization, was highly expressed in macular Müller cells. Furthermore, EPPK1 knockout in a human Müller cell-derived cell line led to a decrease in traction forces as well as to changes in cell size, shape, and filopodia characteristics. We here identified EPPK1 as a central molecular player in the region-specific architecture of the human retina, which likely enables specific functions under the immense mechanical loads in vivo.

AB - The human macula is a highly specialized retinal region with pit-like morphology and rich in cones. How Müller cells, the principal glial cell type in the retina, are adapted to this environment is still poorly understood. We compared proteomic data from cone- and rod-rich retinae from human and mice and identified different expression profiles of cone- and rod-associated Müller cells that converged on pathways representing extracellular matrix and cell adhesion. In particular, epiplakin (EPPK1), which is thought to play a role in intermediate filament organization, was highly expressed in macular Müller cells. Furthermore, EPPK1 knockout in a human Müller cell-derived cell line led to a decrease in traction forces as well as to changes in cell size, shape, and filopodia characteristics. We here identified EPPK1 as a central molecular player in the region-specific architecture of the human retina, which likely enables specific functions under the immense mechanical loads in vivo.

U2 - 10.1002/glia.24283

DO - 10.1002/glia.24283

M3 - SCORING: Journal article

C2 - 36334068

VL - 71

SP - 391

EP - 414

JO - GLIA

JF - GLIA

SN - 0894-1491

IS - 2

ER -