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Resting membrane potential is less negative in trabeculae from right atrial appendages of women, but action potential duration does not shorten with age

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Resting membrane potential is less negative in trabeculae from right atrial appendages of women, but action potential duration does not shorten with age. / Pecha, Simon; Ismaili, Djemail; Geelhoed, Bastiaan; Knaut, Michael; Reichenspurner, Hermann; Eschenhagen, Thomas; Schnabel, Renate B; Christ, Torsten; Ravens, Ursula.

In: J MOL CELL CARDIOL, Vol. 176, 03.2023, p. 1-10.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Pecha, S, Ismaili, D, Geelhoed, B, Knaut, M, Reichenspurner, H, Eschenhagen, T, Schnabel, RB, Christ, T & Ravens, U 2023, 'Resting membrane potential is less negative in trabeculae from right atrial appendages of women, but action potential duration does not shorten with age', J MOL CELL CARDIOL, vol. 176, pp. 1-10. https://doi.org/10.1016/j.yjmcc.2023.01.006

APA

Pecha, S., Ismaili, D., Geelhoed, B., Knaut, M., Reichenspurner, H., Eschenhagen, T., Schnabel, R. B., Christ, T., & Ravens, U. (2023). Resting membrane potential is less negative in trabeculae from right atrial appendages of women, but action potential duration does not shorten with age. J MOL CELL CARDIOL, 176, 1-10. https://doi.org/10.1016/j.yjmcc.2023.01.006

Vancouver

Pecha S, Ismaili D, Geelhoed B, Knaut M, Reichenspurner H, Eschenhagen T et al. Resting membrane potential is less negative in trabeculae from right atrial appendages of women, but action potential duration does not shorten with age. J MOL CELL CARDIOL. 2023 Mar;176:1-10. https://doi.org/10.1016/j.yjmcc.2023.01.006

Bibtex

@article{ce2841ef600f410e8c4e1370df8af1ea,
title = "Resting membrane potential is less negative in trabeculae from right atrial appendages of women, but action potential duration does not shorten with age",
abstract = "AIMS: The incidence of atrial fibrillation (AF) increases with age. Women have a lower risk. Little is known on the impact of age, sex and clinical variables on action potentials (AP) recorded in right atrial tissue obtained during open heart surgery from patients in sinus rhythm (SR) and in longstanding AF. We here investigated whether age or sex have an impact on the shape of AP recorded in vitro from right atrial tissue.METHODS: We performed multivariable analysis of individual AP data from trabeculae obtained during heart surgery of patients in SR (n = 320) or in longstanding AF (n = 201). AP were recorded by sharp microelectrodes at 37 °C at 1 Hz. Impact of clinical variables were modeled using a multivariable mixed model regression.RESULTS: In SR, AP duration at 90% repolarization (APD90) increased with age. Lower ejection fraction and higher body mass index were associated with smaller action potential amplitude (APA) and maximum upstroke velocity (Vmax). The use of beta-blockers was associated with larger APD90. In tissues from women, resting membrane potential was less negative and APA as well as Vmax were smaller. Besides shorter APD20 in elderly patients, effects of age and sex on atrial AP were lost in AF.CONCLUSION: The higher probability to develop AF at advanced age cannot be explained by a shortening in APD90. Less negative RMP and lower upstroke velocity might contribute to lower incidence of AF in women, which may be of clinical relevance.",
keywords = "Humans, Female, Aged, Action Potentials, Atrial Fibrillation, Atrial Appendage, Membrane Potentials, Heart Atria",
author = "Simon Pecha and Djemail Ismaili and Bastiaan Geelhoed and Michael Knaut and Hermann Reichenspurner and Thomas Eschenhagen and Schnabel, {Renate B} and Torsten Christ and Ursula Ravens",
note = "Copyright {\textcopyright} 2023. Published by Elsevier Ltd.",
year = "2023",
month = mar,
doi = "10.1016/j.yjmcc.2023.01.006",
language = "English",
volume = "176",
pages = "1--10",
journal = "J MOL CELL CARDIOL",
issn = "0022-2828",
publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Resting membrane potential is less negative in trabeculae from right atrial appendages of women, but action potential duration does not shorten with age

AU - Pecha, Simon

AU - Ismaili, Djemail

AU - Geelhoed, Bastiaan

AU - Knaut, Michael

AU - Reichenspurner, Hermann

AU - Eschenhagen, Thomas

AU - Schnabel, Renate B

AU - Christ, Torsten

AU - Ravens, Ursula

N1 - Copyright © 2023. Published by Elsevier Ltd.

PY - 2023/3

Y1 - 2023/3

N2 - AIMS: The incidence of atrial fibrillation (AF) increases with age. Women have a lower risk. Little is known on the impact of age, sex and clinical variables on action potentials (AP) recorded in right atrial tissue obtained during open heart surgery from patients in sinus rhythm (SR) and in longstanding AF. We here investigated whether age or sex have an impact on the shape of AP recorded in vitro from right atrial tissue.METHODS: We performed multivariable analysis of individual AP data from trabeculae obtained during heart surgery of patients in SR (n = 320) or in longstanding AF (n = 201). AP were recorded by sharp microelectrodes at 37 °C at 1 Hz. Impact of clinical variables were modeled using a multivariable mixed model regression.RESULTS: In SR, AP duration at 90% repolarization (APD90) increased with age. Lower ejection fraction and higher body mass index were associated with smaller action potential amplitude (APA) and maximum upstroke velocity (Vmax). The use of beta-blockers was associated with larger APD90. In tissues from women, resting membrane potential was less negative and APA as well as Vmax were smaller. Besides shorter APD20 in elderly patients, effects of age and sex on atrial AP were lost in AF.CONCLUSION: The higher probability to develop AF at advanced age cannot be explained by a shortening in APD90. Less negative RMP and lower upstroke velocity might contribute to lower incidence of AF in women, which may be of clinical relevance.

AB - AIMS: The incidence of atrial fibrillation (AF) increases with age. Women have a lower risk. Little is known on the impact of age, sex and clinical variables on action potentials (AP) recorded in right atrial tissue obtained during open heart surgery from patients in sinus rhythm (SR) and in longstanding AF. We here investigated whether age or sex have an impact on the shape of AP recorded in vitro from right atrial tissue.METHODS: We performed multivariable analysis of individual AP data from trabeculae obtained during heart surgery of patients in SR (n = 320) or in longstanding AF (n = 201). AP were recorded by sharp microelectrodes at 37 °C at 1 Hz. Impact of clinical variables were modeled using a multivariable mixed model regression.RESULTS: In SR, AP duration at 90% repolarization (APD90) increased with age. Lower ejection fraction and higher body mass index were associated with smaller action potential amplitude (APA) and maximum upstroke velocity (Vmax). The use of beta-blockers was associated with larger APD90. In tissues from women, resting membrane potential was less negative and APA as well as Vmax were smaller. Besides shorter APD20 in elderly patients, effects of age and sex on atrial AP were lost in AF.CONCLUSION: The higher probability to develop AF at advanced age cannot be explained by a shortening in APD90. Less negative RMP and lower upstroke velocity might contribute to lower incidence of AF in women, which may be of clinical relevance.

KW - Humans

KW - Female

KW - Aged

KW - Action Potentials

KW - Atrial Fibrillation

KW - Atrial Appendage

KW - Membrane Potentials

KW - Heart Atria

U2 - 10.1016/j.yjmcc.2023.01.006

DO - 10.1016/j.yjmcc.2023.01.006

M3 - SCORING: Journal article

C2 - 36681268

VL - 176

SP - 1

EP - 10

JO - J MOL CELL CARDIOL

JF - J MOL CELL CARDIOL

SN - 0022-2828

ER -