Requirement of secondary lymphoid tissues for the induction of primary and secondary T cell responses against Listeria monocytogenes.
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Requirement of secondary lymphoid tissues for the induction of primary and secondary T cell responses against Listeria monocytogenes. / Kursar, Mischo; Jänner, Nathalie; Pfeffer, Klaus; Brinkmann, Volker; Kaufmann, Stefan H E; Mittrücker, Hans Willi.
In: EUR J IMMUNOL, Vol. 38, No. 1, 1, 2008, p. 127-138.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Requirement of secondary lymphoid tissues for the induction of primary and secondary T cell responses against Listeria monocytogenes.
AU - Kursar, Mischo
AU - Jänner, Nathalie
AU - Pfeffer, Klaus
AU - Brinkmann, Volker
AU - Kaufmann, Stefan H E
AU - Mittrücker, Hans Willi
PY - 2008
Y1 - 2008
N2 - Activation of naive T cells is tightly controlled and depends on cognate interactions with professional antigen-presenting cells. We analyzed dependency on secondary lymphoid tissues for the activation of naive and memory CD4(+) and CD8(+) T cells following primary and secondary Listeria monocytogenes infection, respectively. In splenectomized lymphotoxin-beta receptor-deficient mice, lacking all secondary lymphoid tissues, oral infection with L. monocytogenes failed to induce bacteria-specific CD4(+) and CD8(+) T cell responses. Treatment of splenectomized wild-type mice with FTY720, a drug that prevents egress of T cells from lymph nodes, also reduced T cell responses after oral L. monocytogenes infection and blocked T cell responses after intravenous infection. FTY720-treated wild-type and lymphotoxin-beta receptor-deficient mice show only slightly impaired recall responses. However, T cell responses were profoundly inhibited when mice were splenectomized subsequently to recovery from primary infection. T cell transfer experiments demonstrated that the impaired secondary T cell response was not simply due to removal of a large fraction of memory T cells by splenectomy. Overall, these results indicate that not only primary T cell responses, but also secondary T cell responses, highly depend on the lymphoid environment for effective activation.
AB - Activation of naive T cells is tightly controlled and depends on cognate interactions with professional antigen-presenting cells. We analyzed dependency on secondary lymphoid tissues for the activation of naive and memory CD4(+) and CD8(+) T cells following primary and secondary Listeria monocytogenes infection, respectively. In splenectomized lymphotoxin-beta receptor-deficient mice, lacking all secondary lymphoid tissues, oral infection with L. monocytogenes failed to induce bacteria-specific CD4(+) and CD8(+) T cell responses. Treatment of splenectomized wild-type mice with FTY720, a drug that prevents egress of T cells from lymph nodes, also reduced T cell responses after oral L. monocytogenes infection and blocked T cell responses after intravenous infection. FTY720-treated wild-type and lymphotoxin-beta receptor-deficient mice show only slightly impaired recall responses. However, T cell responses were profoundly inhibited when mice were splenectomized subsequently to recovery from primary infection. T cell transfer experiments demonstrated that the impaired secondary T cell response was not simply due to removal of a large fraction of memory T cells by splenectomy. Overall, these results indicate that not only primary T cell responses, but also secondary T cell responses, highly depend on the lymphoid environment for effective activation.
M3 - SCORING: Zeitschriftenaufsatz
VL - 38
SP - 127
EP - 138
JO - EUR J IMMUNOL
JF - EUR J IMMUNOL
SN - 0014-2980
IS - 1
M1 - 1
ER -
