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Relaxin-like factor: a highly specific and constitutive new marker for Leydig cells in the human testis.

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Relaxin-like factor: a highly specific and constitutive new marker for Leydig cells in the human testis. / Ivell, R; Balvers, M; Domagalski, R; Ungefroren, H; Hunt, N; Schulze, Wolfgang.

In: MOL HUM REPROD, Vol. 3, No. 6, 6, 1997, p. 459-466.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Ivell, R, Balvers, M, Domagalski, R, Ungefroren, H, Hunt, N & Schulze, W 1997, 'Relaxin-like factor: a highly specific and constitutive new marker for Leydig cells in the human testis.', MOL HUM REPROD, vol. 3, no. 6, 6, pp. 459-466. <http://www.ncbi.nlm.nih.gov/pubmed/9239733?dopt=Citation>

APA

Ivell, R., Balvers, M., Domagalski, R., Ungefroren, H., Hunt, N., & Schulze, W. (1997). Relaxin-like factor: a highly specific and constitutive new marker for Leydig cells in the human testis. MOL HUM REPROD, 3(6), 459-466. [6]. http://www.ncbi.nlm.nih.gov/pubmed/9239733?dopt=Citation

Vancouver

Ivell R, Balvers M, Domagalski R, Ungefroren H, Hunt N, Schulze W. Relaxin-like factor: a highly specific and constitutive new marker for Leydig cells in the human testis. MOL HUM REPROD. 1997;3(6):459-466. 6.

Bibtex

@article{a697e31c239c460aa8f9eed05056d43b,
title = "Relaxin-like factor: a highly specific and constitutive new marker for Leydig cells in the human testis.",
abstract = "The complete protein-coding region of the human relaxin-like factor (RLF; formerly Ley-I-L) was cloned by reverse transcription-polymerase chain reaction from human testis and subcloned into a bacterial expression plasmid for the production of recombinant human RLF in Escherichia coli. Polyclonal antibodies were raised against the recombinant RLF, as well as against a peptide epitope from the B-domain of the RLF polypeptide. Antibodies were used for immunohistochemistry of Bouin-fixed, paraffin-embedded samples of human testis tissues. Specific immunoreactivity was located exclusively in the Leydig cells with a consistent high intensity of staining, showing similar spatial distribution to other Leydig cell markers, such as the luteinizing hormone (LH) receptor and 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), and to the pattern of RLF mRNA shown by in-situ transcript hybridization. In biopsy samples from patients with severe disturbances of spermatogenesis, RLF staining intensity was consistently high in all cases, unlike staining for 3 beta-HSD which varied considerably between patients. Immunostaining for RLF would thus appear to offer an interesting new marker for Leydig cells in human testis samples.",
author = "R Ivell and M Balvers and R Domagalski and H Ungefroren and N Hunt and Wolfgang Schulze",
year = "1997",
language = "Deutsch",
volume = "3",
pages = "459--466",
journal = "MOL HUM REPROD",
issn = "1360-9947",
publisher = "Oxford University Press",
number = "6",

}

RIS

TY - JOUR

T1 - Relaxin-like factor: a highly specific and constitutive new marker for Leydig cells in the human testis.

AU - Ivell, R

AU - Balvers, M

AU - Domagalski, R

AU - Ungefroren, H

AU - Hunt, N

AU - Schulze, Wolfgang

PY - 1997

Y1 - 1997

N2 - The complete protein-coding region of the human relaxin-like factor (RLF; formerly Ley-I-L) was cloned by reverse transcription-polymerase chain reaction from human testis and subcloned into a bacterial expression plasmid for the production of recombinant human RLF in Escherichia coli. Polyclonal antibodies were raised against the recombinant RLF, as well as against a peptide epitope from the B-domain of the RLF polypeptide. Antibodies were used for immunohistochemistry of Bouin-fixed, paraffin-embedded samples of human testis tissues. Specific immunoreactivity was located exclusively in the Leydig cells with a consistent high intensity of staining, showing similar spatial distribution to other Leydig cell markers, such as the luteinizing hormone (LH) receptor and 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), and to the pattern of RLF mRNA shown by in-situ transcript hybridization. In biopsy samples from patients with severe disturbances of spermatogenesis, RLF staining intensity was consistently high in all cases, unlike staining for 3 beta-HSD which varied considerably between patients. Immunostaining for RLF would thus appear to offer an interesting new marker for Leydig cells in human testis samples.

AB - The complete protein-coding region of the human relaxin-like factor (RLF; formerly Ley-I-L) was cloned by reverse transcription-polymerase chain reaction from human testis and subcloned into a bacterial expression plasmid for the production of recombinant human RLF in Escherichia coli. Polyclonal antibodies were raised against the recombinant RLF, as well as against a peptide epitope from the B-domain of the RLF polypeptide. Antibodies were used for immunohistochemistry of Bouin-fixed, paraffin-embedded samples of human testis tissues. Specific immunoreactivity was located exclusively in the Leydig cells with a consistent high intensity of staining, showing similar spatial distribution to other Leydig cell markers, such as the luteinizing hormone (LH) receptor and 3 beta-hydroxysteroid dehydrogenase (3 beta-HSD), and to the pattern of RLF mRNA shown by in-situ transcript hybridization. In biopsy samples from patients with severe disturbances of spermatogenesis, RLF staining intensity was consistently high in all cases, unlike staining for 3 beta-HSD which varied considerably between patients. Immunostaining for RLF would thus appear to offer an interesting new marker for Leydig cells in human testis samples.

M3 - SCORING: Zeitschriftenaufsatz

VL - 3

SP - 459

EP - 466

JO - MOL HUM REPROD

JF - MOL HUM REPROD

SN - 1360-9947

IS - 6

M1 - 6

ER -