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Regulation of Pseudomonas aeruginosa hemF and hemN by the dual action of the redox response regulators Anr and Dnr

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Regulation of Pseudomonas aeruginosa hemF and hemN by the dual action of the redox response regulators Anr and Dnr. / Rompf, A; Hungerer, C; Hoffmann, T; Lindenmeyer, M; Römling, U; Gross, U; Doss, M O; Arai, H; Igarashi, Y; Jahn, D.

In: MOL MICROBIOL, Vol. 29, No. 4, 08.1998, p. 985-97.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Rompf, A, Hungerer, C, Hoffmann, T, Lindenmeyer, M, Römling, U, Gross, U, Doss, MO, Arai, H, Igarashi, Y & Jahn, D 1998, 'Regulation of Pseudomonas aeruginosa hemF and hemN by the dual action of the redox response regulators Anr and Dnr', MOL MICROBIOL, vol. 29, no. 4, pp. 985-97.

APA

Rompf, A., Hungerer, C., Hoffmann, T., Lindenmeyer, M., Römling, U., Gross, U., Doss, M. O., Arai, H., Igarashi, Y., & Jahn, D. (1998). Regulation of Pseudomonas aeruginosa hemF and hemN by the dual action of the redox response regulators Anr and Dnr. MOL MICROBIOL, 29(4), 985-97.

Vancouver

Rompf A, Hungerer C, Hoffmann T, Lindenmeyer M, Römling U, Gross U et al. Regulation of Pseudomonas aeruginosa hemF and hemN by the dual action of the redox response regulators Anr and Dnr. MOL MICROBIOL. 1998 Aug;29(4):985-97.

Bibtex

@article{c42293c841444749b0ababa1e088e2ae,
title = "Regulation of Pseudomonas aeruginosa hemF and hemN by the dual action of the redox response regulators Anr and Dnr",
abstract = "The oxidative decarboxylation of coproporphyrinogen III catalysed by an oxygen-dependent oxidase (HemF) and an oxygen-independent dehydrogenase (HemN) is one of the key regulatory points of haem biosynthesis in Pseudomonas aeruginosa. To investigate the oxygen-dependent regulation of hemF and hemN, the corresponding genes were cloned from the P. aeruginosa chromosome. Recognition sequences for the Fnr-type transcriptional regulator Anr were detected -44.5 bp from the 5' end of the hemF mRNA transcript and at an optimal distance of -41.5 bp with respect to the transcriptional start of hemN. An approximately 10-fold anaerobic induction of hemN gene expression was mediated by the dual action of Anr and a second Fnr-type regulator, Dnr. Regulation by both proteins required the Anr recognition sequence. Surprisingly, aerobic expression of hemN was dependent only on Anr. An anr mutant did not contain detectable amounts of hemN mRNA and accumulated coproporphyrin III both aerobically and anaerobically, indicating the importance of HemN for aerobic and anaerobic haem formation. Mutation of hemN and hemF did not abolish aerobic or anaerobic growth, indicating the existence of an additional HemN-type enzyme, which was termed HemZ. Expression of hemF was induced approximately 20-fold during anaerobic growth and, as was found for hemN, both Anr and Dnr were required for anaerobic induction. Paradoxically, oxygen is necessary for HemF catalysis, suggesting the existence of an additional physiological function for the P. aeruginosa HemF protein.",
keywords = "Aerobiosis, Amino Acid Sequence, Anaerobiosis, Bacterial Proteins, Base Sequence, Chromosome Mapping, Cloning, Molecular, Coproporphyrinogen Oxidase, Coproporphyrinogens, DNA Primers, DNA, Bacterial, DNA-Binding Proteins, Gene Expression Regulation, Bacterial, Genes, Bacterial, Molecular Sequence Data, Mutation, Oxidation-Reduction, Pseudomonas aeruginosa, RNA, Bacterial, RNA, Messenger, Trans-Activators, Transcription Factors, Journal Article, Research Support, Non-U.S. Gov't",
author = "A Rompf and C Hungerer and T Hoffmann and M Lindenmeyer and U R{\"o}mling and U Gross and Doss, {M O} and H Arai and Y Igarashi and D Jahn",
year = "1998",
month = aug,
language = "English",
volume = "29",
pages = "985--97",
journal = "MOL MICROBIOL",
issn = "0950-382X",
publisher = "Wiley-Blackwell",
number = "4",

}

RIS

TY - JOUR

T1 - Regulation of Pseudomonas aeruginosa hemF and hemN by the dual action of the redox response regulators Anr and Dnr

AU - Rompf, A

AU - Hungerer, C

AU - Hoffmann, T

AU - Lindenmeyer, M

AU - Römling, U

AU - Gross, U

AU - Doss, M O

AU - Arai, H

AU - Igarashi, Y

AU - Jahn, D

PY - 1998/8

Y1 - 1998/8

N2 - The oxidative decarboxylation of coproporphyrinogen III catalysed by an oxygen-dependent oxidase (HemF) and an oxygen-independent dehydrogenase (HemN) is one of the key regulatory points of haem biosynthesis in Pseudomonas aeruginosa. To investigate the oxygen-dependent regulation of hemF and hemN, the corresponding genes were cloned from the P. aeruginosa chromosome. Recognition sequences for the Fnr-type transcriptional regulator Anr were detected -44.5 bp from the 5' end of the hemF mRNA transcript and at an optimal distance of -41.5 bp with respect to the transcriptional start of hemN. An approximately 10-fold anaerobic induction of hemN gene expression was mediated by the dual action of Anr and a second Fnr-type regulator, Dnr. Regulation by both proteins required the Anr recognition sequence. Surprisingly, aerobic expression of hemN was dependent only on Anr. An anr mutant did not contain detectable amounts of hemN mRNA and accumulated coproporphyrin III both aerobically and anaerobically, indicating the importance of HemN for aerobic and anaerobic haem formation. Mutation of hemN and hemF did not abolish aerobic or anaerobic growth, indicating the existence of an additional HemN-type enzyme, which was termed HemZ. Expression of hemF was induced approximately 20-fold during anaerobic growth and, as was found for hemN, both Anr and Dnr were required for anaerobic induction. Paradoxically, oxygen is necessary for HemF catalysis, suggesting the existence of an additional physiological function for the P. aeruginosa HemF protein.

AB - The oxidative decarboxylation of coproporphyrinogen III catalysed by an oxygen-dependent oxidase (HemF) and an oxygen-independent dehydrogenase (HemN) is one of the key regulatory points of haem biosynthesis in Pseudomonas aeruginosa. To investigate the oxygen-dependent regulation of hemF and hemN, the corresponding genes were cloned from the P. aeruginosa chromosome. Recognition sequences for the Fnr-type transcriptional regulator Anr were detected -44.5 bp from the 5' end of the hemF mRNA transcript and at an optimal distance of -41.5 bp with respect to the transcriptional start of hemN. An approximately 10-fold anaerobic induction of hemN gene expression was mediated by the dual action of Anr and a second Fnr-type regulator, Dnr. Regulation by both proteins required the Anr recognition sequence. Surprisingly, aerobic expression of hemN was dependent only on Anr. An anr mutant did not contain detectable amounts of hemN mRNA and accumulated coproporphyrin III both aerobically and anaerobically, indicating the importance of HemN for aerobic and anaerobic haem formation. Mutation of hemN and hemF did not abolish aerobic or anaerobic growth, indicating the existence of an additional HemN-type enzyme, which was termed HemZ. Expression of hemF was induced approximately 20-fold during anaerobic growth and, as was found for hemN, both Anr and Dnr were required for anaerobic induction. Paradoxically, oxygen is necessary for HemF catalysis, suggesting the existence of an additional physiological function for the P. aeruginosa HemF protein.

KW - Aerobiosis

KW - Amino Acid Sequence

KW - Anaerobiosis

KW - Bacterial Proteins

KW - Base Sequence

KW - Chromosome Mapping

KW - Cloning, Molecular

KW - Coproporphyrinogen Oxidase

KW - Coproporphyrinogens

KW - DNA Primers

KW - DNA, Bacterial

KW - DNA-Binding Proteins

KW - Gene Expression Regulation, Bacterial

KW - Genes, Bacterial

KW - Molecular Sequence Data

KW - Mutation

KW - Oxidation-Reduction

KW - Pseudomonas aeruginosa

KW - RNA, Bacterial

KW - RNA, Messenger

KW - Trans-Activators

KW - Transcription Factors

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

M3 - SCORING: Journal article

C2 - 9767567

VL - 29

SP - 985

EP - 997

JO - MOL MICROBIOL

JF - MOL MICROBIOL

SN - 0950-382X

IS - 4

ER -