Reference intervals for plasma L-arginine and the L-arginine:asymmetric dimethylarginine ratio in the Framingham Offspring Cohort.
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Reference intervals for plasma L-arginine and the L-arginine:asymmetric dimethylarginine ratio in the Framingham Offspring Cohort. / Lüneburg, Nicole; Xanthakis, Vanessa; Schwedhelm, Edzard; Sullivan, Lisa M; Maas, Renke; Anderssohn, Maike; Riederer, Ulrich; Glazer, Nicole L; Vasan, Ramachandran S; Böger, Rainer.
In: J NUTR, Vol. 141, No. 12, 12, 2011, p. 2186-2190.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Reference intervals for plasma L-arginine and the L-arginine:asymmetric dimethylarginine ratio in the Framingham Offspring Cohort.
AU - Lüneburg, Nicole
AU - Xanthakis, Vanessa
AU - Schwedhelm, Edzard
AU - Sullivan, Lisa M
AU - Maas, Renke
AU - Anderssohn, Maike
AU - Riederer, Ulrich
AU - Glazer, Nicole L
AU - Vasan, Ramachandran S
AU - Böger, Rainer
PY - 2011
Y1 - 2011
N2 - L-arginine, as a precursor of NO synthesis, has attracted much scientific attention in recent years. Experimental mouse models suggest that L-arginine supplementation can retard, halt, or even reverse atherogenesis. In human studies, supplementation with L-arginine improved endothelium-dependent vasodilation. However, L-arginine levels are best interpreted in the context of levels of asymmetric dimethylarginine (ADMA), a competitive inhibitor of NO synthase. Thus, reference limits for circulating L-arginine and the L-arginine:ADMA ratio may help to determine the nutritional state of individuals at high cardiovascular risk in light of increased ADMA levels. We defined reference limits for plasma L-arginine in 1141 people and for the L-arginine:ADMA ratio in 1138 relatively healthy individuals from the Framingham Offspring Cohort. Plasma L-arginine and ADMA concentrations were determined by using a stable isotope-based LC-MS/MS method. The reference limits (2.5th and 97.5th percentiles) for plasma L-arginine were 41.0 ?mol/L (95% CI = 39.5-42.5 ?mol/L) and 114 ?mol/L (95% CI = 112-115 ?mol/L), whereas corresponding reference limits (2.5th and 97.5th percentiles) for the L-arginine:ADMA ratio were 74.3 ?mol/L (95% CI = 71.1-77.3 ?mol/L) and 225 ?mol/L (95% CI = 222-228 ?mol/L). Plasma L-arginine was positively associated with the estimated glomerular filtration rate (eGFR) and blood glucose levels, whereas the L-arginine:ADMA ratio was positively associated with eGFR and diastolic blood pressure but inversely associated with homocysteine and (log)C-reactive protein. We report reference levels for plasma L-arginine and for the L-arginine:ADMA ratio that may be helpful for evaluation of the effects of L-arginine supplementation in participants with an impaired L-arginine/NO pathway.
AB - L-arginine, as a precursor of NO synthesis, has attracted much scientific attention in recent years. Experimental mouse models suggest that L-arginine supplementation can retard, halt, or even reverse atherogenesis. In human studies, supplementation with L-arginine improved endothelium-dependent vasodilation. However, L-arginine levels are best interpreted in the context of levels of asymmetric dimethylarginine (ADMA), a competitive inhibitor of NO synthase. Thus, reference limits for circulating L-arginine and the L-arginine:ADMA ratio may help to determine the nutritional state of individuals at high cardiovascular risk in light of increased ADMA levels. We defined reference limits for plasma L-arginine in 1141 people and for the L-arginine:ADMA ratio in 1138 relatively healthy individuals from the Framingham Offspring Cohort. Plasma L-arginine and ADMA concentrations were determined by using a stable isotope-based LC-MS/MS method. The reference limits (2.5th and 97.5th percentiles) for plasma L-arginine were 41.0 ?mol/L (95% CI = 39.5-42.5 ?mol/L) and 114 ?mol/L (95% CI = 112-115 ?mol/L), whereas corresponding reference limits (2.5th and 97.5th percentiles) for the L-arginine:ADMA ratio were 74.3 ?mol/L (95% CI = 71.1-77.3 ?mol/L) and 225 ?mol/L (95% CI = 222-228 ?mol/L). Plasma L-arginine was positively associated with the estimated glomerular filtration rate (eGFR) and blood glucose levels, whereas the L-arginine:ADMA ratio was positively associated with eGFR and diastolic blood pressure but inversely associated with homocysteine and (log)C-reactive protein. We report reference levels for plasma L-arginine and for the L-arginine:ADMA ratio that may be helpful for evaluation of the effects of L-arginine supplementation in participants with an impaired L-arginine/NO pathway.
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Cross-Sectional Studies
KW - Glomerular Filtration Rate
KW - Reference Values
KW - Chromatography, Liquid
KW - Tandem Mass Spectrometry
KW - Arginine/analogs & derivatives/blood/metabolism
KW - Dietary Supplements
KW - Endothelium-Dependent Relaxing Factors/metabolism
KW - Massachusetts
KW - Nitric Oxide Synthase/antagonists & inhibitors
KW - Humans
KW - Male
KW - Aged
KW - Female
KW - Middle Aged
KW - Cross-Sectional Studies
KW - Glomerular Filtration Rate
KW - Reference Values
KW - Chromatography, Liquid
KW - Tandem Mass Spectrometry
KW - Arginine/analogs & derivatives/blood/metabolism
KW - Dietary Supplements
KW - Endothelium-Dependent Relaxing Factors/metabolism
KW - Massachusetts
KW - Nitric Oxide Synthase/antagonists & inhibitors
M3 - SCORING: Journal article
VL - 141
SP - 2186
EP - 2190
JO - J NUTR
JF - J NUTR
SN - 0022-3166
IS - 12
M1 - 12
ER -
