Recycling of apoprotein E is associated with cholesterol efflux and high density lipoprotein internalization.

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Recycling of apoprotein E is associated with cholesterol efflux and high density lipoprotein internalization. / Heeren, Joerg; Grewal, Thomas; Laatsch, Alexander; Rottke, Daniel; Rinninger, Franz; Enrich, Carlos; Beisiegel, Ulrike.

In: J BIOL CHEM, Vol. 278, No. 16, 16, 2003, p. 14370-14378.

Research output: SCORING: Contribution to journalSCORING: Journal articleResearchpeer-review

Harvard

Heeren, J, Grewal, T, Laatsch, A, Rottke, D, Rinninger, F, Enrich, C & Beisiegel, U 2003, 'Recycling of apoprotein E is associated with cholesterol efflux and high density lipoprotein internalization.', J BIOL CHEM, vol. 278, no. 16, 16, pp. 14370-14378. <http://www.ncbi.nlm.nih.gov/pubmed/12584196?dopt=Citation>

APA

Heeren, J., Grewal, T., Laatsch, A., Rottke, D., Rinninger, F., Enrich, C., & Beisiegel, U. (2003). Recycling of apoprotein E is associated with cholesterol efflux and high density lipoprotein internalization. J BIOL CHEM, 278(16), 14370-14378. [16]. http://www.ncbi.nlm.nih.gov/pubmed/12584196?dopt=Citation

Vancouver

Heeren J, Grewal T, Laatsch A, Rottke D, Rinninger F, Enrich C et al. Recycling of apoprotein E is associated with cholesterol efflux and high density lipoprotein internalization. J BIOL CHEM. 2003;278(16):14370-14378. 16.

Bibtex

@article{cedc7faaee944deabc580d0b72425f15,
title = "Recycling of apoprotein E is associated with cholesterol efflux and high density lipoprotein internalization.",
abstract = "After receptor-mediated endocytosis of triglyceride-rich lipoproteins (TRL) into the liver, TRL particles are immediately disintegrated in peripheral endosomal compartments. Whereas core lipids and apoprotein B are delivered for degradation into lysosomes, TRL-derived apoE is efficiently recycled back to the plasma membrane. This is followed by apoE re-secretion and association of apoE with high density lipoproteins (HDL). Because HDL and apoE can independently promote cholesterol efflux, we investigated whether recycling of TRL-derived apoE in human hepatoma cells and fibroblasts could be linked to intracellular cholesterol transport. In this study we demonstrate that HDL(3) does not only act as an extracellular acceptor for recycled apoE but also stimulates the recycling of internalized TRL-derived apoE. Furthermore, radioactive pulse-chase experiments indicate that apoE recycling is accompanied by cholesterol efflux. Confocal imaging reveals co-localization of apoE and cholesterol in early endosome antigen 1-positive endosomes. During apoE re-secretion, HDL(3)-derived apoA-I is found in these early endosome antigen 1, cholesterol-containing endosomes. As shown by time-lapse fluorescence microscopy, apoE recycling involves the intracellular trafficking of apoA-I to pre-existing and TRL-derived apoE/cholesterol-containing endosomes in the periphery. Thus, these studies provide evidence for a new intracellular link between TRL-derived apoE, cellular cholesterol transport, and HDL metabolism.",
author = "Joerg Heeren and Thomas Grewal and Alexander Laatsch and Daniel Rottke and Franz Rinninger and Carlos Enrich and Ulrike Beisiegel",
year = "2003",
language = "Deutsch",
volume = "278",
pages = "14370--14378",
journal = "J BIOL CHEM",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "16",

}

RIS

TY - JOUR

T1 - Recycling of apoprotein E is associated with cholesterol efflux and high density lipoprotein internalization.

AU - Heeren, Joerg

AU - Grewal, Thomas

AU - Laatsch, Alexander

AU - Rottke, Daniel

AU - Rinninger, Franz

AU - Enrich, Carlos

AU - Beisiegel, Ulrike

PY - 2003

Y1 - 2003

N2 - After receptor-mediated endocytosis of triglyceride-rich lipoproteins (TRL) into the liver, TRL particles are immediately disintegrated in peripheral endosomal compartments. Whereas core lipids and apoprotein B are delivered for degradation into lysosomes, TRL-derived apoE is efficiently recycled back to the plasma membrane. This is followed by apoE re-secretion and association of apoE with high density lipoproteins (HDL). Because HDL and apoE can independently promote cholesterol efflux, we investigated whether recycling of TRL-derived apoE in human hepatoma cells and fibroblasts could be linked to intracellular cholesterol transport. In this study we demonstrate that HDL(3) does not only act as an extracellular acceptor for recycled apoE but also stimulates the recycling of internalized TRL-derived apoE. Furthermore, radioactive pulse-chase experiments indicate that apoE recycling is accompanied by cholesterol efflux. Confocal imaging reveals co-localization of apoE and cholesterol in early endosome antigen 1-positive endosomes. During apoE re-secretion, HDL(3)-derived apoA-I is found in these early endosome antigen 1, cholesterol-containing endosomes. As shown by time-lapse fluorescence microscopy, apoE recycling involves the intracellular trafficking of apoA-I to pre-existing and TRL-derived apoE/cholesterol-containing endosomes in the periphery. Thus, these studies provide evidence for a new intracellular link between TRL-derived apoE, cellular cholesterol transport, and HDL metabolism.

AB - After receptor-mediated endocytosis of triglyceride-rich lipoproteins (TRL) into the liver, TRL particles are immediately disintegrated in peripheral endosomal compartments. Whereas core lipids and apoprotein B are delivered for degradation into lysosomes, TRL-derived apoE is efficiently recycled back to the plasma membrane. This is followed by apoE re-secretion and association of apoE with high density lipoproteins (HDL). Because HDL and apoE can independently promote cholesterol efflux, we investigated whether recycling of TRL-derived apoE in human hepatoma cells and fibroblasts could be linked to intracellular cholesterol transport. In this study we demonstrate that HDL(3) does not only act as an extracellular acceptor for recycled apoE but also stimulates the recycling of internalized TRL-derived apoE. Furthermore, radioactive pulse-chase experiments indicate that apoE recycling is accompanied by cholesterol efflux. Confocal imaging reveals co-localization of apoE and cholesterol in early endosome antigen 1-positive endosomes. During apoE re-secretion, HDL(3)-derived apoA-I is found in these early endosome antigen 1, cholesterol-containing endosomes. As shown by time-lapse fluorescence microscopy, apoE recycling involves the intracellular trafficking of apoA-I to pre-existing and TRL-derived apoE/cholesterol-containing endosomes in the periphery. Thus, these studies provide evidence for a new intracellular link between TRL-derived apoE, cellular cholesterol transport, and HDL metabolism.

M3 - SCORING: Zeitschriftenaufsatz

VL - 278

SP - 14370

EP - 14378

JO - J BIOL CHEM

JF - J BIOL CHEM

SN - 0021-9258

IS - 16

M1 - 16

ER -