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Recycling of apolipoprotein E is not associated with cholesterol efflux in neuronal cells.

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Recycling of apolipoprotein E is not associated with cholesterol efflux in neuronal cells. / Rellin, Lars; Heeren, Jörg; Beisiegel, Ulrike.

In: BBA-MOL CELL BIOL L, Vol. 1781, No. 5, 5, 2008, p. 232-238.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Rellin, L, Heeren, J & Beisiegel, U 2008, 'Recycling of apolipoprotein E is not associated with cholesterol efflux in neuronal cells.', BBA-MOL CELL BIOL L, vol. 1781, no. 5, 5, pp. 232-238. <http://www.ncbi.nlm.nih.gov/pubmed/18359298?dopt=Citation>

APA

Rellin, L., Heeren, J., & Beisiegel, U. (2008). Recycling of apolipoprotein E is not associated with cholesterol efflux in neuronal cells. BBA-MOL CELL BIOL L, 1781(5), 232-238. [5]. http://www.ncbi.nlm.nih.gov/pubmed/18359298?dopt=Citation

Vancouver

Rellin L, Heeren J, Beisiegel U. Recycling of apolipoprotein E is not associated with cholesterol efflux in neuronal cells. BBA-MOL CELL BIOL L. 2008;1781(5):232-238. 5.

Bibtex

@article{0d23eac1dc424e78800088c993493218,
title = "Recycling of apolipoprotein E is not associated with cholesterol efflux in neuronal cells.",
abstract = "After receptor-mediated endocytosis of apolipoprotein E (apoE)-containing lipoproteins in hepatocytes, the isoform apoE3 is efficiently recycled in a process which is associated with cholesterol efflux. Recycling and cholesterol efflux are greatly reduced when apoE4 is the only isoform present. ApoE is the main apolipoprotein in cerebrospinal fluid, and it plays a pivotal role in maintaining cholesterol homeostasis in the brain. The isoform apoE4 is associated with an increased risk of Alzheimer's disease and it has been postulated that high intracellular cholesterol levels promote the amyloidogenic processing of amyloid precursor protein. Therefore we investigated the cellular processing of different apoE isoforms as well as the associated cholesterol efflux in the murine neuronal cell line HT-22. Uptake of apoE3-containing lipoproteins resulted in the expected recycling while, as seen in non-neuronal cells, recycling of apoE4 was significantly reduced. However, despite these differences in apoE recycling, there was no difference in rates of cholesterol efflux. Therefore we conclude that in this neuronal cell model the reduced recycling of apoE4 does not affect cellular cholesterol metabolism.",
author = "Lars Rellin and J{\"o}rg Heeren and Ulrike Beisiegel",
year = "2008",
language = "Deutsch",
volume = "1781",
pages = "232--238",
journal = "BBA-MOL CELL BIOL L",
issn = "1388-1981",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Recycling of apolipoprotein E is not associated with cholesterol efflux in neuronal cells.

AU - Rellin, Lars

AU - Heeren, Jörg

AU - Beisiegel, Ulrike

PY - 2008

Y1 - 2008

N2 - After receptor-mediated endocytosis of apolipoprotein E (apoE)-containing lipoproteins in hepatocytes, the isoform apoE3 is efficiently recycled in a process which is associated with cholesterol efflux. Recycling and cholesterol efflux are greatly reduced when apoE4 is the only isoform present. ApoE is the main apolipoprotein in cerebrospinal fluid, and it plays a pivotal role in maintaining cholesterol homeostasis in the brain. The isoform apoE4 is associated with an increased risk of Alzheimer's disease and it has been postulated that high intracellular cholesterol levels promote the amyloidogenic processing of amyloid precursor protein. Therefore we investigated the cellular processing of different apoE isoforms as well as the associated cholesterol efflux in the murine neuronal cell line HT-22. Uptake of apoE3-containing lipoproteins resulted in the expected recycling while, as seen in non-neuronal cells, recycling of apoE4 was significantly reduced. However, despite these differences in apoE recycling, there was no difference in rates of cholesterol efflux. Therefore we conclude that in this neuronal cell model the reduced recycling of apoE4 does not affect cellular cholesterol metabolism.

AB - After receptor-mediated endocytosis of apolipoprotein E (apoE)-containing lipoproteins in hepatocytes, the isoform apoE3 is efficiently recycled in a process which is associated with cholesterol efflux. Recycling and cholesterol efflux are greatly reduced when apoE4 is the only isoform present. ApoE is the main apolipoprotein in cerebrospinal fluid, and it plays a pivotal role in maintaining cholesterol homeostasis in the brain. The isoform apoE4 is associated with an increased risk of Alzheimer's disease and it has been postulated that high intracellular cholesterol levels promote the amyloidogenic processing of amyloid precursor protein. Therefore we investigated the cellular processing of different apoE isoforms as well as the associated cholesterol efflux in the murine neuronal cell line HT-22. Uptake of apoE3-containing lipoproteins resulted in the expected recycling while, as seen in non-neuronal cells, recycling of apoE4 was significantly reduced. However, despite these differences in apoE recycling, there was no difference in rates of cholesterol efflux. Therefore we conclude that in this neuronal cell model the reduced recycling of apoE4 does not affect cellular cholesterol metabolism.

M3 - SCORING: Zeitschriftenaufsatz

VL - 1781

SP - 232

EP - 238

JO - BBA-MOL CELL BIOL L

JF - BBA-MOL CELL BIOL L

SN - 1388-1981

IS - 5

M1 - 5

ER -