Recombinase polymerase amplification and lateral flow assay for ultrasensitive detection of low-density Plasmodium falciparum infection from controlled human malaria infection studies and naturally acquired infections

Albert Lalremruata; The Trong Nguyen; Matthew B.B. McCall; Ghyslain Mombo-Ngoma; Selidji T. Agnandji; Ayôla A. Adegnika; Bertrand Lell; Michael Ramharter; Stephen L. Hoffman; Peter G. Kremsner; Benjamin Mordmüller

doi:10.1128/JCM.01879-19

Recombinase polymerase amplification and lateral flow assay for ultrasensitive detection of low-density Plasmodium falciparum infection from controlled human malaria infection studies and naturally acquired infections

Standard

Recombinase polymerase amplification and lateral flow assay for ultrasensitive detection of low-density Plasmodium falciparum infection from controlled human malaria infection studies and naturally acquired infections. / Lalremruata, Albert; Nguyen, The Trong; McCall, Matthew B.B.; Mombo-Ngoma, Ghyslain; Agnandji, Selidji T.; Adegnika, Ayôla A.; Lell, Bertrand; Ramharter, Michael; Hoffman, Stephen L.; Kremsner, Peter G.; Mordmüller, Benjamin.

In: Journal of Clinical Microbiology, Vol. 58, No. 5, e01879-19, 23.04.2020.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Lalremruata, A, Nguyen, TT, McCall, MBB, Mombo-Ngoma, G, Agnandji, ST, Adegnika, AA, Lell, B, Ramharter, M, Hoffman, SL, Kremsner, PG & Mordmüller, B 2020, 'Recombinase polymerase amplification and lateral flow assay for ultrasensitive detection of low-density Plasmodium falciparum infection from controlled human malaria infection studies and naturally acquired infections', Journal of Clinical Microbiology, vol. 58, no. 5, e01879-19. https://doi.org/10.1128/JCM.01879-19

APA

Lalremruata, A., Nguyen, T. T., McCall, M. B. B., Mombo-Ngoma, G., Agnandji, S. T., Adegnika, A. A., Lell, B., Ramharter, M., Hoffman, S. L., Kremsner, P. G., & Mordmüller, B. (2020). Recombinase polymerase amplification and lateral flow assay for ultrasensitive detection of low-density Plasmodium falciparum infection from controlled human malaria infection studies and naturally acquired infections. Journal of Clinical Microbiology, 58(5), [e01879-19]. https://doi.org/10.1128/JCM.01879-19

Vancouver

Lalremruata A, Nguyen TT, McCall MBB, Mombo-Ngoma G, Agnandji ST, Adegnika AA et al. Recombinase polymerase amplification and lateral flow assay for ultrasensitive detection of low-density Plasmodium falciparum infection from controlled human malaria infection studies and naturally acquired infections. Journal of Clinical Microbiology. 2020 Apr 23;58(5). e01879-19. https://doi.org/10.1128/JCM.01879-19

Bibtex

@article{357ff87471864596a8f01aff4ea385e0,

title = "Recombinase polymerase amplification and lateral flow assay for ultrasensitive detection of low-density Plasmodium falciparum infection from controlled human malaria infection studies and naturally acquired infections",

abstract = "Microscopy and rapid diagnostic tests (RDTs) are the main diagnostic tools for malaria but fail to detect low-density parasitemias that are important for maintaining malaria transmission. To complement existing diagnostic methods, an isothermal reverse transcription-recombinase polymerase amplification and lateral flow assay (RT-RPA) was developed. We compared the performance with that of ultrasensitive reverse transcription-quantitative PCR (uRT-qPCR) using nucleic acid extracts from blood samples (n=114) obtained after standardized controlled human malaria infection (CHMI) with Plasmodium falciparum sporozoites. As a preliminary investigation, we also sampled asymptomatic individuals (n=28) in an area of malaria endemicity (Lambar{\'e}n{\'e}, Gabon) to validate RT-RPA and assess its performance with unprocessed blood samples (dbRT-RPA). In 114 samples analyzed from CHMI trials, the positive percent agreement to uRT-qPCR was 90% (95% confidence interval [CI], 80 to 96). The negative percent agreement was 100% (95% CI, 92 to 100). The lower limit of detection was 64 parasites/ml. In Gabon, RT-RPA was 100% accurate with asymptomatic volunteers (n=28), while simplified dbRT-RPA showed 89% accuracy. In a subgroup analysis, RT-RPA detected 9/10 RT-qPCR-positive samples, while loop-mediated isothermal amplification (LAMP) detected 2/10. RT-RPA is a reliable diagnostic test for asymptomatic low-density infections. It is particularly useful in settings where uRT-qPCR is difficult to implement.",

keywords = "CHMI, Diagnostics, Elimination, Isothermal, Mass screen and treat, Plasmodium falciparum, Submicroscopic, Malaria, Falciparum/diagnosis, Plasmodium falciparum/genetics, Humans, Recombinases, Malaria/diagnosis, Nucleic Acid Amplification Techniques, Gabon, Molecular Diagnostic Techniques, Sensitivity and Specificity",

author = "Albert Lalremruata and Nguyen, {The Trong} and McCall, {Matthew B.B.} and Ghyslain Mombo-Ngoma and Agnandji, {Selidji T.} and Adegnika, {Ay{\^o}la A.} and Bertrand Lell and Michael Ramharter and Hoffman, {Stephen L.} and Kremsner, {Peter G.} and Benjamin Mordm{\"u}ller",

note = "Copyright {\textcopyright} 2020 American Society for Microbiology.",

year = "2020",

month = apr,

day = "23",

doi = "10.1128/JCM.01879-19",

language = "English",

volume = "58",

journal = "J CLIN MICROBIOL",

issn = "0095-1137",

publisher = "American Society for Microbiology",

number = "5",

}

RIS

TY - JOUR

T1 - Recombinase polymerase amplification and lateral flow assay for ultrasensitive detection of low-density Plasmodium falciparum infection from controlled human malaria infection studies and naturally acquired infections

AU - Lalremruata, Albert

AU - Nguyen, The Trong

AU - McCall, Matthew B.B.

AU - Mombo-Ngoma, Ghyslain

AU - Agnandji, Selidji T.

AU - Adegnika, Ayôla A.

AU - Lell, Bertrand

AU - Ramharter, Michael

AU - Hoffman, Stephen L.

AU - Kremsner, Peter G.

AU - Mordmüller, Benjamin

PY - 2020/4/23

Y1 - 2020/4/23

N2 - Microscopy and rapid diagnostic tests (RDTs) are the main diagnostic tools for malaria but fail to detect low-density parasitemias that are important for maintaining malaria transmission. To complement existing diagnostic methods, an isothermal reverse transcription-recombinase polymerase amplification and lateral flow assay (RT-RPA) was developed. We compared the performance with that of ultrasensitive reverse transcription-quantitative PCR (uRT-qPCR) using nucleic acid extracts from blood samples (n=114) obtained after standardized controlled human malaria infection (CHMI) with Plasmodium falciparum sporozoites. As a preliminary investigation, we also sampled asymptomatic individuals (n=28) in an area of malaria endemicity (Lambaréné, Gabon) to validate RT-RPA and assess its performance with unprocessed blood samples (dbRT-RPA). In 114 samples analyzed from CHMI trials, the positive percent agreement to uRT-qPCR was 90% (95% confidence interval [CI], 80 to 96). The negative percent agreement was 100% (95% CI, 92 to 100). The lower limit of detection was 64 parasites/ml. In Gabon, RT-RPA was 100% accurate with asymptomatic volunteers (n=28), while simplified dbRT-RPA showed 89% accuracy. In a subgroup analysis, RT-RPA detected 9/10 RT-qPCR-positive samples, while loop-mediated isothermal amplification (LAMP) detected 2/10. RT-RPA is a reliable diagnostic test for asymptomatic low-density infections. It is particularly useful in settings where uRT-qPCR is difficult to implement.

AB - Microscopy and rapid diagnostic tests (RDTs) are the main diagnostic tools for malaria but fail to detect low-density parasitemias that are important for maintaining malaria transmission. To complement existing diagnostic methods, an isothermal reverse transcription-recombinase polymerase amplification and lateral flow assay (RT-RPA) was developed. We compared the performance with that of ultrasensitive reverse transcription-quantitative PCR (uRT-qPCR) using nucleic acid extracts from blood samples (n=114) obtained after standardized controlled human malaria infection (CHMI) with Plasmodium falciparum sporozoites. As a preliminary investigation, we also sampled asymptomatic individuals (n=28) in an area of malaria endemicity (Lambaréné, Gabon) to validate RT-RPA and assess its performance with unprocessed blood samples (dbRT-RPA). In 114 samples analyzed from CHMI trials, the positive percent agreement to uRT-qPCR was 90% (95% confidence interval [CI], 80 to 96). The negative percent agreement was 100% (95% CI, 92 to 100). The lower limit of detection was 64 parasites/ml. In Gabon, RT-RPA was 100% accurate with asymptomatic volunteers (n=28), while simplified dbRT-RPA showed 89% accuracy. In a subgroup analysis, RT-RPA detected 9/10 RT-qPCR-positive samples, while loop-mediated isothermal amplification (LAMP) detected 2/10. RT-RPA is a reliable diagnostic test for asymptomatic low-density infections. It is particularly useful in settings where uRT-qPCR is difficult to implement.

KW - CHMI

KW - Diagnostics

KW - Elimination

KW - Isothermal

KW - Mass screen and treat

KW - Plasmodium falciparum

KW - Submicroscopic

KW - Malaria, Falciparum/diagnosis

KW - Plasmodium falciparum/genetics

KW - Humans

KW - Recombinases

KW - Malaria/diagnosis

KW - Nucleic Acid Amplification Techniques

KW - Gabon

KW - Molecular Diagnostic Techniques

KW - Sensitivity and Specificity

UR - http://www.scopus.com/inward/record.url?scp=85084027252&partnerID=8YFLogxK

U2 - 10.1128/JCM.01879-19

DO - 10.1128/JCM.01879-19

M3 - SCORING: Journal article

C2 - 32102854

AN - SCOPUS:85084027252

VL - 58

JO - J CLIN MICROBIOL

JF - J CLIN MICROBIOL

SN - 0095-1137

IS - 5

M1 - e01879-19

ER -