Real-time quantitative Y chromosome-specific PCR (QYCS-PCR) for monitoring hematopoietic chimerism after sex-mismatched allogeneic stem cell transplantation.
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Real-time quantitative Y chromosome-specific PCR (QYCS-PCR) for monitoring hematopoietic chimerism after sex-mismatched allogeneic stem cell transplantation. / Fehse, B; Chukhlovin, A; Kühlcke, K; Marinetz, O; Vorwig, O; Renges, H; Krüger, W; Zabelina, Tatjana; Dudina, O; Finckenstein, F G; Kröger, N; Kabisch, H; Hochhaus, A; Zander, A R.
In: J HEMATOTH STEM CELL, Vol. 10, No. 3, 3, 2001, p. 419-425.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Real-time quantitative Y chromosome-specific PCR (QYCS-PCR) for monitoring hematopoietic chimerism after sex-mismatched allogeneic stem cell transplantation.
AU - Fehse, B
AU - Chukhlovin, A
AU - Kühlcke, K
AU - Marinetz, O
AU - Vorwig, O
AU - Renges, H
AU - Krüger, W
AU - Zabelina, Tatjana
AU - Dudina, O
AU - Finckenstein, F G
AU - Kröger, N
AU - Kabisch, H
AU - Hochhaus, A
AU - Zander, A R
PY - 2001
Y1 - 2001
N2 - Y chromosome-specific sequences can be used to detect remaining male cells after sex-mismatched allogeneic blood stem cell transplantation (HSCT) involving a male patient and female donor, which represents approximately 25% of all cases. We developed a quantitative Y chromosome-specific PCR assay (QYCS-PCR) based on the DFFRY gene for the determination of hematopoietic donor chimerism. We analyzed blood and marrow samples from more than 40 patients at various time points after both standard and nonmyeloablative allogeneic HSCT. We found that real-time PCR combines extreme sensitivity, with a detection level of less than 1 male in 100,000 female cells (
AB - Y chromosome-specific sequences can be used to detect remaining male cells after sex-mismatched allogeneic blood stem cell transplantation (HSCT) involving a male patient and female donor, which represents approximately 25% of all cases. We developed a quantitative Y chromosome-specific PCR assay (QYCS-PCR) based on the DFFRY gene for the determination of hematopoietic donor chimerism. We analyzed blood and marrow samples from more than 40 patients at various time points after both standard and nonmyeloablative allogeneic HSCT. We found that real-time PCR combines extreme sensitivity, with a detection level of less than 1 male in 100,000 female cells (
M3 - SCORING: Zeitschriftenaufsatz
VL - 10
SP - 419
EP - 425
JO - J HEMATOTH STEM CELL
JF - J HEMATOTH STEM CELL
SN - 1525-8165
IS - 3
M1 - 3
ER -