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Random peptide libraries displayed on adeno-associated virus to select for targeted gene therapy vectors

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Random peptide libraries displayed on adeno-associated virus to select for targeted gene therapy vectors. / Müller, Oliver J; Kaul, Felix; Weitzman, Matthew D; Pasqualini, Renata; Arap, Wadih; Kleinschmidt, Jürgen A; Trepel, Martin.

In: NAT BIOTECHNOL, Vol. 21, No. 9, 09.2003, p. 1040-6.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Müller, OJ, Kaul, F, Weitzman, MD, Pasqualini, R, Arap, W, Kleinschmidt, JA & Trepel, M 2003, 'Random peptide libraries displayed on adeno-associated virus to select for targeted gene therapy vectors', NAT BIOTECHNOL, vol. 21, no. 9, pp. 1040-6. https://doi.org/10.1038/nbt856

APA

Müller, O. J., Kaul, F., Weitzman, M. D., Pasqualini, R., Arap, W., Kleinschmidt, J. A., & Trepel, M. (2003). Random peptide libraries displayed on adeno-associated virus to select for targeted gene therapy vectors. NAT BIOTECHNOL, 21(9), 1040-6. https://doi.org/10.1038/nbt856

Vancouver

Müller OJ, Kaul F, Weitzman MD, Pasqualini R, Arap W, Kleinschmidt JA et al. Random peptide libraries displayed on adeno-associated virus to select for targeted gene therapy vectors. NAT BIOTECHNOL. 2003 Sep;21(9):1040-6. https://doi.org/10.1038/nbt856

Bibtex

@article{ce4427e398b643f185e565c840951e9b,
title = "Random peptide libraries displayed on adeno-associated virus to select for targeted gene therapy vectors",
abstract = "Characterizing the molecular diversity of the cell surface is critical for targeting gene therapy. Cell type-specific binding ligands can be used to target gene therapy vectors. However, targeting systems in which optimum eukaryotic vectors can be selected on the cells of interest are not available. Here, we introduce and validate a random adeno-associated virus (AAV) peptide library in which each virus particle displays a random peptide at the capsid surface. This library was generated in a three-step system that ensures encoding of displayed peptides by the packaged DNA. As proof-of-concept, we screened AAV-libraries on human coronary artery endothelial cells. We observed selection of particular peptide motifs. The selected peptides enhanced transduction in coronary endothelial cells but not in control nonendothelial cells. This vector targeting strategy has advantages over other combinatorial approaches such as phage display because selection occurs within the context of the capsid and may have a broad range of applications in biotechnology and medicine.",
keywords = "Amino Acid Sequence, Capsid, Cells, Cultured, Coronary Vessels, Dependovirus, Endothelium, Vascular, Gene Expression Regulation, Viral, Gene Targeting, Gene Transfer Techniques, Genetic Therapy, Genetic Vectors, HeLa Cells, Humans, Molecular Sequence Data, Peptide Library, Sequence Analysis, Protein, Transduction, Genetic, Viral Proteins, Comparative Study, Evaluation Studies, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S., Validation Studies",
author = "M{\"u}ller, {Oliver J} and Felix Kaul and Weitzman, {Matthew D} and Renata Pasqualini and Wadih Arap and Kleinschmidt, {J{\"u}rgen A} and Martin Trepel",
year = "2003",
month = sep,
doi = "10.1038/nbt856",
language = "English",
volume = "21",
pages = "1040--6",
journal = "NAT BIOTECHNOL",
issn = "1087-0156",
publisher = "NATURE PUBLISHING GROUP",
number = "9",

}

RIS

TY - JOUR

T1 - Random peptide libraries displayed on adeno-associated virus to select for targeted gene therapy vectors

AU - Müller, Oliver J

AU - Kaul, Felix

AU - Weitzman, Matthew D

AU - Pasqualini, Renata

AU - Arap, Wadih

AU - Kleinschmidt, Jürgen A

AU - Trepel, Martin

PY - 2003/9

Y1 - 2003/9

N2 - Characterizing the molecular diversity of the cell surface is critical for targeting gene therapy. Cell type-specific binding ligands can be used to target gene therapy vectors. However, targeting systems in which optimum eukaryotic vectors can be selected on the cells of interest are not available. Here, we introduce and validate a random adeno-associated virus (AAV) peptide library in which each virus particle displays a random peptide at the capsid surface. This library was generated in a three-step system that ensures encoding of displayed peptides by the packaged DNA. As proof-of-concept, we screened AAV-libraries on human coronary artery endothelial cells. We observed selection of particular peptide motifs. The selected peptides enhanced transduction in coronary endothelial cells but not in control nonendothelial cells. This vector targeting strategy has advantages over other combinatorial approaches such as phage display because selection occurs within the context of the capsid and may have a broad range of applications in biotechnology and medicine.

AB - Characterizing the molecular diversity of the cell surface is critical for targeting gene therapy. Cell type-specific binding ligands can be used to target gene therapy vectors. However, targeting systems in which optimum eukaryotic vectors can be selected on the cells of interest are not available. Here, we introduce and validate a random adeno-associated virus (AAV) peptide library in which each virus particle displays a random peptide at the capsid surface. This library was generated in a three-step system that ensures encoding of displayed peptides by the packaged DNA. As proof-of-concept, we screened AAV-libraries on human coronary artery endothelial cells. We observed selection of particular peptide motifs. The selected peptides enhanced transduction in coronary endothelial cells but not in control nonendothelial cells. This vector targeting strategy has advantages over other combinatorial approaches such as phage display because selection occurs within the context of the capsid and may have a broad range of applications in biotechnology and medicine.

KW - Amino Acid Sequence

KW - Capsid

KW - Cells, Cultured

KW - Coronary Vessels

KW - Dependovirus

KW - Endothelium, Vascular

KW - Gene Expression Regulation, Viral

KW - Gene Targeting

KW - Gene Transfer Techniques

KW - Genetic Therapy

KW - Genetic Vectors

KW - HeLa Cells

KW - Humans

KW - Molecular Sequence Data

KW - Peptide Library

KW - Sequence Analysis, Protein

KW - Transduction, Genetic

KW - Viral Proteins

KW - Comparative Study

KW - Evaluation Studies

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

KW - Research Support, U.S. Gov't, Non-P.H.S.

KW - Research Support, U.S. Gov't, P.H.S.

KW - Validation Studies

U2 - 10.1038/nbt856

DO - 10.1038/nbt856

M3 - SCORING: Journal article

C2 - 12897791

VL - 21

SP - 1040

EP - 1046

JO - NAT BIOTECHNOL

JF - NAT BIOTECHNOL

SN - 1087-0156

IS - 9

ER -