Radial somatic F-actin organization affects growth cone dynamics during early neuronal development

TY - JOUR

T1 - Radial somatic F-actin organization affects growth cone dynamics during early neuronal development

AU - Meka, Durga Praveen

AU - Scharrenberg, Robin

AU - Zhao, Bing

AU - Kobler, Oliver

AU - König, Theresa

AU - Schaefer, Irina

AU - Schwanke, Birgit

AU - Klykov, Sergei

AU - Richter, Melanie

AU - Eggert, Dennis

AU - Windhorst, Sabine

AU - Dotti, Carlos G

AU - Kreutz, Michael R

AU - Mikhaylova, Marina

AU - Calderon de Anda, Froylan

N1 - © 2019 The Authors. Published under the terms of the CC BY 4.0 license.

PY - 2019/12/5

Y1 - 2019/12/5

N2 - The centrosome is thought to be the major neuronal microtubule-organizing center (MTOC) in early neuronal development, producing microtubules with a radial organization. In addition, albeit in vitro, recent work showed that isolated centrosomes could serve as an actin-organizing center, raising the possibility that neuronal development may, in addition, require a centrosome-based actin radial organization. Here, we report, using super-resolution microscopy and live-cell imaging of cultured rodent neurons, F-actin organization around the centrosome with dynamic F-actin aster-like structures with F-actin fibers extending and retracting actively. Photoactivation/photoconversion experiments and molecular manipulations of F-actin stability reveal a robust flux of somatic F-actin toward the cell periphery. Finally, we show that somatic F-actin intermingles with centrosomal PCM-1 (pericentriolar material 1 protein) satellites. Knockdown of PCM-1 and disruption of centrosomal activity not only affect F-actin dynamics near the centrosome but also in distal growth cones. Collectively, the data show a radial F-actin organization during early neuronal development, which might be a cellular mechanism for providing peripheral regions with a fast and continuous source of actin polymers, hence sustaining initial neuronal development.

AB - The centrosome is thought to be the major neuronal microtubule-organizing center (MTOC) in early neuronal development, producing microtubules with a radial organization. In addition, albeit in vitro, recent work showed that isolated centrosomes could serve as an actin-organizing center, raising the possibility that neuronal development may, in addition, require a centrosome-based actin radial organization. Here, we report, using super-resolution microscopy and live-cell imaging of cultured rodent neurons, F-actin organization around the centrosome with dynamic F-actin aster-like structures with F-actin fibers extending and retracting actively. Photoactivation/photoconversion experiments and molecular manipulations of F-actin stability reveal a robust flux of somatic F-actin toward the cell periphery. Finally, we show that somatic F-actin intermingles with centrosomal PCM-1 (pericentriolar material 1 protein) satellites. Knockdown of PCM-1 and disruption of centrosomal activity not only affect F-actin dynamics near the centrosome but also in distal growth cones. Collectively, the data show a radial F-actin organization during early neuronal development, which might be a cellular mechanism for providing peripheral regions with a fast and continuous source of actin polymers, hence sustaining initial neuronal development.

U2 - 10.15252/embr.201947743

DO - 10.15252/embr.201947743

M3 - SCORING: Journal article

C2 - 31650708

VL - 20

SP - e47743

JO - EMBO REP

JF - EMBO REP

SN - 1469-221X

IS - 12

ER -