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RabGEFs are a major determinant for specific Rab membrane targeting

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RabGEFs are a major determinant for specific Rab membrane targeting. / Blümer, Julia; Rey, Juliana; Dehmelt, Leif; Mazel, Tomáš; Wu, Yao-Wen; Bastiaens, Philippe; Goody, Roger S; Itzen, Aymelt.

In: J CELL BIOL, Vol. 200, No. 3, 04.02.2013, p. 287-300.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Blümer, J, Rey, J, Dehmelt, L, Mazel, T, Wu, Y-W, Bastiaens, P, Goody, RS & Itzen, A 2013, 'RabGEFs are a major determinant for specific Rab membrane targeting', J CELL BIOL, vol. 200, no. 3, pp. 287-300. https://doi.org/10.1083/jcb.201209113

APA

Blümer, J., Rey, J., Dehmelt, L., Mazel, T., Wu, Y-W., Bastiaens, P., Goody, R. S., & Itzen, A. (2013). RabGEFs are a major determinant for specific Rab membrane targeting. J CELL BIOL, 200(3), 287-300. https://doi.org/10.1083/jcb.201209113

Vancouver

Blümer J, Rey J, Dehmelt L, Mazel T, Wu Y-W, Bastiaens P et al. RabGEFs are a major determinant for specific Rab membrane targeting. J CELL BIOL. 2013 Feb 4;200(3):287-300. https://doi.org/10.1083/jcb.201209113

Bibtex

@article{a0dba919646540398bdca083cc4945fb,
title = "RabGEFs are a major determinant for specific Rab membrane targeting",
abstract = "Eukaryotic cells critically depend on the correct regulation of intracellular vesicular trafficking to transport biological material. The Rab subfamily of small guanosine triphosphatases controls these processes by acting as a molecular on/off switch. To fulfill their function, active Rab proteins need to localize to intracellular membranes via posttranslationally attached geranylgeranyl lipids. Each member of the manifold Rab family localizes specifically to a distinct membrane, but it is unclear how this specific membrane recruitment is achieved. Here, we demonstrate that Rab-activating guanosine diphosphate/guanosine triphosphate exchange factors (GEFs) display the minimal targeting machinery for recruiting Rabs from the cytosol to the correct membrane using the Rab-GEF pairs Rab5A-Rabex-5, Rab1A-DrrA, and Rab8-Rabin8 as model systems. Specific mistargeting of Rabex-5/DrrA/Rabin8 to mitochondria led to catalytic recruitment of Rab5A/Rab1A/Rab8A in a time-dependent manner that required the catalytic activity of the GEF. Therefore, RabGEFs are major determinants for specific Rab membrane targeting.",
keywords = "Animals, COS Cells, Cell Membrane, Cercopithecus aethiops, Guanine Nucleotide Exchange Factors, Humans, Microscopy, Confocal, Mitochondria, Mitochondrial Membranes, Models, Biological, Mutant Proteins, Protein Structure, Tertiary, Protein Transport, Subcellular Fractions, rab GTP-Binding Proteins, rab5 GTP-Binding Proteins, Journal Article, Research Support, Non-U.S. Gov't",
author = "Julia Bl{\"u}mer and Juliana Rey and Leif Dehmelt and Tom{\'a}{\v s} Mazel and Yao-Wen Wu and Philippe Bastiaens and Goody, {Roger S} and Aymelt Itzen",
year = "2013",
month = feb,
day = "4",
doi = "10.1083/jcb.201209113",
language = "English",
volume = "200",
pages = "287--300",
journal = "J CELL BIOL",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "3",

}

RIS

TY - JOUR

T1 - RabGEFs are a major determinant for specific Rab membrane targeting

AU - Blümer, Julia

AU - Rey, Juliana

AU - Dehmelt, Leif

AU - Mazel, Tomáš

AU - Wu, Yao-Wen

AU - Bastiaens, Philippe

AU - Goody, Roger S

AU - Itzen, Aymelt

PY - 2013/2/4

Y1 - 2013/2/4

N2 - Eukaryotic cells critically depend on the correct regulation of intracellular vesicular trafficking to transport biological material. The Rab subfamily of small guanosine triphosphatases controls these processes by acting as a molecular on/off switch. To fulfill their function, active Rab proteins need to localize to intracellular membranes via posttranslationally attached geranylgeranyl lipids. Each member of the manifold Rab family localizes specifically to a distinct membrane, but it is unclear how this specific membrane recruitment is achieved. Here, we demonstrate that Rab-activating guanosine diphosphate/guanosine triphosphate exchange factors (GEFs) display the minimal targeting machinery for recruiting Rabs from the cytosol to the correct membrane using the Rab-GEF pairs Rab5A-Rabex-5, Rab1A-DrrA, and Rab8-Rabin8 as model systems. Specific mistargeting of Rabex-5/DrrA/Rabin8 to mitochondria led to catalytic recruitment of Rab5A/Rab1A/Rab8A in a time-dependent manner that required the catalytic activity of the GEF. Therefore, RabGEFs are major determinants for specific Rab membrane targeting.

AB - Eukaryotic cells critically depend on the correct regulation of intracellular vesicular trafficking to transport biological material. The Rab subfamily of small guanosine triphosphatases controls these processes by acting as a molecular on/off switch. To fulfill their function, active Rab proteins need to localize to intracellular membranes via posttranslationally attached geranylgeranyl lipids. Each member of the manifold Rab family localizes specifically to a distinct membrane, but it is unclear how this specific membrane recruitment is achieved. Here, we demonstrate that Rab-activating guanosine diphosphate/guanosine triphosphate exchange factors (GEFs) display the minimal targeting machinery for recruiting Rabs from the cytosol to the correct membrane using the Rab-GEF pairs Rab5A-Rabex-5, Rab1A-DrrA, and Rab8-Rabin8 as model systems. Specific mistargeting of Rabex-5/DrrA/Rabin8 to mitochondria led to catalytic recruitment of Rab5A/Rab1A/Rab8A in a time-dependent manner that required the catalytic activity of the GEF. Therefore, RabGEFs are major determinants for specific Rab membrane targeting.

KW - Animals

KW - COS Cells

KW - Cell Membrane

KW - Cercopithecus aethiops

KW - Guanine Nucleotide Exchange Factors

KW - Humans

KW - Microscopy, Confocal

KW - Mitochondria

KW - Mitochondrial Membranes

KW - Models, Biological

KW - Mutant Proteins

KW - Protein Structure, Tertiary

KW - Protein Transport

KW - Subcellular Fractions

KW - rab GTP-Binding Proteins

KW - rab5 GTP-Binding Proteins

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1083/jcb.201209113

DO - 10.1083/jcb.201209113

M3 - SCORING: Journal article

C2 - 23382462

VL - 200

SP - 287

EP - 300

JO - J CELL BIOL

JF - J CELL BIOL

SN - 0021-9525

IS - 3

ER -