Quantitative Map of Proteome Dynamics during Neuronal Differentiation

Christian K Frese; Marina Mikhaylova; Riccardo Stucchi; Violette Gautier; Qingyang Liu; Shabaz Mohammed; Albert J R Heck; A F Maarten Altelaar; Casper C Hoogenraad

doi:10.1016/j.celrep.2017.01.025

Quantitative Map of Proteome Dynamics during Neuronal Differentiation

Standard

Quantitative Map of Proteome Dynamics during Neuronal Differentiation. / Frese, Christian K; Mikhaylova, Marina; Stucchi, Riccardo; Gautier, Violette; Liu, Qingyang; Mohammed, Shabaz; Heck, Albert J R; Altelaar, A F Maarten; Hoogenraad, Casper C.

In: CELL REP, Vol. 18, No. 6, 07.02.2017, p. 1527-1542.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Frese, CK, Mikhaylova, M, Stucchi, R, Gautier, V, Liu, Q, Mohammed, S, Heck, AJR, Altelaar, AFM & Hoogenraad, CC 2017, 'Quantitative Map of Proteome Dynamics during Neuronal Differentiation', CELL REP, vol. 18, no. 6, pp. 1527-1542. https://doi.org/10.1016/j.celrep.2017.01.025

APA

Frese, C. K., Mikhaylova, M., Stucchi, R., Gautier, V., Liu, Q., Mohammed, S., Heck, A. J. R., Altelaar, A. F. M., & Hoogenraad, C. C. (2017). Quantitative Map of Proteome Dynamics during Neuronal Differentiation. CELL REP, 18(6), 1527-1542. https://doi.org/10.1016/j.celrep.2017.01.025

Vancouver

Frese CK, Mikhaylova M, Stucchi R, Gautier V, Liu Q, Mohammed S et al. Quantitative Map of Proteome Dynamics during Neuronal Differentiation. CELL REP. 2017 Feb 7;18(6):1527-1542. https://doi.org/10.1016/j.celrep.2017.01.025

Bibtex

@article{29f89b08404349768f218f78392c4497,

title = "Quantitative Map of Proteome Dynamics during Neuronal Differentiation",

abstract = "Neuronal differentiation is a multistep process that shapes and re-shapes neurons by progressing through several typical stages, including axon outgrowth, dendritogenesis, and synapse formation. To systematically profile proteome dynamics throughout neuronal differentiation, we took cultured rat hippocampal neurons at different developmental stages and monitored changes in protein abundance using a combination of stable isotope labeling and high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS). Almost one third of all 4,500 proteins quantified underwent a more than 2-fold expression change during neuronal differentiation, indicating extensive remodeling of the neuron proteome. To highlight the strength of our resource, we studied the neural-cell-adhesion molecule 1 (NCAM1) and found that it stimulates dendritic arbor development by promoting actin filament growth at the dendritic growth cone. We anticipate that our quantitative map of neuronal proteome dynamics is a rich resource for further analyses of the many identified proteins in various neurodevelopmental processes.",

author = "Frese, {Christian K} and Marina Mikhaylova and Riccardo Stucchi and Violette Gautier and Qingyang Liu and Shabaz Mohammed and Heck, {Albert J R} and Altelaar, {A F Maarten} and Hoogenraad, {Casper C}",

year = "2017",

month = feb,

day = "7",

doi = "10.1016/j.celrep.2017.01.025",

language = "English",

volume = "18",

pages = "1527--1542",

journal = "CELL REP",

issn = "2211-1247",

publisher = "Elsevier",

number = "6",

}

RIS

TY - JOUR

T1 - Quantitative Map of Proteome Dynamics during Neuronal Differentiation

AU - Frese, Christian K

AU - Mikhaylova, Marina

AU - Stucchi, Riccardo

AU - Gautier, Violette

AU - Liu, Qingyang

AU - Mohammed, Shabaz

AU - Heck, Albert J R

AU - Altelaar, A F Maarten

AU - Hoogenraad, Casper C

PY - 2017/2/7

Y1 - 2017/2/7

N2 - Neuronal differentiation is a multistep process that shapes and re-shapes neurons by progressing through several typical stages, including axon outgrowth, dendritogenesis, and synapse formation. To systematically profile proteome dynamics throughout neuronal differentiation, we took cultured rat hippocampal neurons at different developmental stages and monitored changes in protein abundance using a combination of stable isotope labeling and high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS). Almost one third of all 4,500 proteins quantified underwent a more than 2-fold expression change during neuronal differentiation, indicating extensive remodeling of the neuron proteome. To highlight the strength of our resource, we studied the neural-cell-adhesion molecule 1 (NCAM1) and found that it stimulates dendritic arbor development by promoting actin filament growth at the dendritic growth cone. We anticipate that our quantitative map of neuronal proteome dynamics is a rich resource for further analyses of the many identified proteins in various neurodevelopmental processes.

AB - Neuronal differentiation is a multistep process that shapes and re-shapes neurons by progressing through several typical stages, including axon outgrowth, dendritogenesis, and synapse formation. To systematically profile proteome dynamics throughout neuronal differentiation, we took cultured rat hippocampal neurons at different developmental stages and monitored changes in protein abundance using a combination of stable isotope labeling and high-resolution liquid chromatography-tandem mass spectrometry (LC-MS/MS). Almost one third of all 4,500 proteins quantified underwent a more than 2-fold expression change during neuronal differentiation, indicating extensive remodeling of the neuron proteome. To highlight the strength of our resource, we studied the neural-cell-adhesion molecule 1 (NCAM1) and found that it stimulates dendritic arbor development by promoting actin filament growth at the dendritic growth cone. We anticipate that our quantitative map of neuronal proteome dynamics is a rich resource for further analyses of the many identified proteins in various neurodevelopmental processes.

U2 - 10.1016/j.celrep.2017.01.025

DO - 10.1016/j.celrep.2017.01.025

M3 - SCORING: Journal article

C2 - 28178528

VL - 18

SP - 1527

EP - 1542

JO - CELL REP

JF - CELL REP

SN - 2211-1247

IS - 6

ER -