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Purification of chemically synthesised dinucleoside(5',5') polyphosphates by displacement chromatography

Standard

Purification of chemically synthesised dinucleoside(5',5') polyphosphates by displacement chromatography. / Jankowski, J; Potthoff, W; Zidek, W; Schlüter, H.

In: Journal of chromatography. B, Biomedical sciences and applications, Vol. 719, No. 1-2, 20.11.1998, p. 63-70.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Jankowski, J, Potthoff, W, Zidek, W & Schlüter, H 1998, 'Purification of chemically synthesised dinucleoside(5',5') polyphosphates by displacement chromatography', Journal of chromatography. B, Biomedical sciences and applications, vol. 719, no. 1-2, pp. 63-70.

APA

Jankowski, J., Potthoff, W., Zidek, W., & Schlüter, H. (1998). Purification of chemically synthesised dinucleoside(5',5') polyphosphates by displacement chromatography. Journal of chromatography. B, Biomedical sciences and applications, 719(1-2), 63-70.

Vancouver

Jankowski J, Potthoff W, Zidek W, Schlüter H. Purification of chemically synthesised dinucleoside(5',5') polyphosphates by displacement chromatography. Journal of chromatography. B, Biomedical sciences and applications. 1998 Nov 20;719(1-2):63-70.

Bibtex

@article{9c93567c82594ed9a68c018a7c0df959,
title = "Purification of chemically synthesised dinucleoside(5',5') polyphosphates by displacement chromatography",
abstract = "Dinucleoside(5',5') polyphosphates (ApnA, ApnG, GpnG, n=3-6) are new group of hormones controlling important biological processes. Because some of the dinucleoside(5',5') polyphosphates are commercially not available purification of chemical synthesised dinucleoside(5',5') polyphosphates became necessary in order to test their physiological and pharmacological properties. It was the aim of this study to find a method which allows purification of 0.1-0.2 g quantities of dinucleoside polyphosphates by analytical HPLC columns yielding products with impurities lower than 1.0%. Adenosine(5')-polyphospho-(5')guanosines were synthesised by mixing the corresponding mononucleotides. The reaction results in a complex mixture of ApnA, ApnG and GpnG (with n=3-6 in all cases). The reaction mixture was concentrated on a preparative C18 reversed-phase column. The concentrate was displaced on a reversed-phase stationary. As a result of displacement chromatography, anion-exchange chromatography in gradient modus yielded baseline separated dinucleoside polyphosphates (homogeneity of the fractions>99%). The identity of the substances were determined by matrix assisted laser desorption ionisation mass spectrometry.",
keywords = "Chromatography, High Pressure Liquid, Chromatography, Ion Exchange, Dinucleoside Phosphates, Molecular Structure, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Journal Article",
author = "J Jankowski and W Potthoff and W Zidek and H Schl{\"u}ter",
year = "1998",
month = nov,
day = "20",
language = "English",
volume = "719",
pages = "63--70",
number = "1-2",

}

RIS

TY - JOUR

T1 - Purification of chemically synthesised dinucleoside(5',5') polyphosphates by displacement chromatography

AU - Jankowski, J

AU - Potthoff, W

AU - Zidek, W

AU - Schlüter, H

PY - 1998/11/20

Y1 - 1998/11/20

N2 - Dinucleoside(5',5') polyphosphates (ApnA, ApnG, GpnG, n=3-6) are new group of hormones controlling important biological processes. Because some of the dinucleoside(5',5') polyphosphates are commercially not available purification of chemical synthesised dinucleoside(5',5') polyphosphates became necessary in order to test their physiological and pharmacological properties. It was the aim of this study to find a method which allows purification of 0.1-0.2 g quantities of dinucleoside polyphosphates by analytical HPLC columns yielding products with impurities lower than 1.0%. Adenosine(5')-polyphospho-(5')guanosines were synthesised by mixing the corresponding mononucleotides. The reaction results in a complex mixture of ApnA, ApnG and GpnG (with n=3-6 in all cases). The reaction mixture was concentrated on a preparative C18 reversed-phase column. The concentrate was displaced on a reversed-phase stationary. As a result of displacement chromatography, anion-exchange chromatography in gradient modus yielded baseline separated dinucleoside polyphosphates (homogeneity of the fractions>99%). The identity of the substances were determined by matrix assisted laser desorption ionisation mass spectrometry.

AB - Dinucleoside(5',5') polyphosphates (ApnA, ApnG, GpnG, n=3-6) are new group of hormones controlling important biological processes. Because some of the dinucleoside(5',5') polyphosphates are commercially not available purification of chemical synthesised dinucleoside(5',5') polyphosphates became necessary in order to test their physiological and pharmacological properties. It was the aim of this study to find a method which allows purification of 0.1-0.2 g quantities of dinucleoside polyphosphates by analytical HPLC columns yielding products with impurities lower than 1.0%. Adenosine(5')-polyphospho-(5')guanosines were synthesised by mixing the corresponding mononucleotides. The reaction results in a complex mixture of ApnA, ApnG and GpnG (with n=3-6 in all cases). The reaction mixture was concentrated on a preparative C18 reversed-phase column. The concentrate was displaced on a reversed-phase stationary. As a result of displacement chromatography, anion-exchange chromatography in gradient modus yielded baseline separated dinucleoside polyphosphates (homogeneity of the fractions>99%). The identity of the substances were determined by matrix assisted laser desorption ionisation mass spectrometry.

KW - Chromatography, High Pressure Liquid

KW - Chromatography, Ion Exchange

KW - Dinucleoside Phosphates

KW - Molecular Structure

KW - Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

KW - Journal Article

M3 - SCORING: Journal article

C2 - 9869365

VL - 719

SP - 63

EP - 70

IS - 1-2

ER -