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Purification, crystallization and preliminary X-ray diffraction analysis of the thiaminase type II from Staphylococcus aureus.

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Purification, crystallization and preliminary X-ray diffraction analysis of the thiaminase type II from Staphylococcus aureus. / Begum, Afshan; Drebes, Julia; Perbandt, Markus; Wrenger, Carsten; Betzel, Christian.

In: ACTA CRYSTALLOGR F, Vol. 67, No. Pt 1, Pt 1, 2011, p. 51-53.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Begum, A, Drebes, J, Perbandt, M, Wrenger, C & Betzel, C 2011, 'Purification, crystallization and preliminary X-ray diffraction analysis of the thiaminase type II from Staphylococcus aureus.', ACTA CRYSTALLOGR F, vol. 67, no. Pt 1, Pt 1, pp. 51-53. <http://www.ncbi.nlm.nih.gov/pubmed/21206023?dopt=Citation>

APA

Begum, A., Drebes, J., Perbandt, M., Wrenger, C., & Betzel, C. (2011). Purification, crystallization and preliminary X-ray diffraction analysis of the thiaminase type II from Staphylococcus aureus. ACTA CRYSTALLOGR F, 67(Pt 1), 51-53. [Pt 1]. http://www.ncbi.nlm.nih.gov/pubmed/21206023?dopt=Citation

Vancouver

Begum A, Drebes J, Perbandt M, Wrenger C, Betzel C. Purification, crystallization and preliminary X-ray diffraction analysis of the thiaminase type II from Staphylococcus aureus. ACTA CRYSTALLOGR F. 2011;67(Pt 1):51-53. Pt 1.

Bibtex

@article{8c524159a443489ebb52b9d824700c6d,
title = "Purification, crystallization and preliminary X-ray diffraction analysis of the thiaminase type II from Staphylococcus aureus.",
abstract = "Thiaminase type II (TenA) catalyzes the deamination of aminopyrimidines, including the cleavage of thiamine to 4-amino-5-hydroxymethyl-2-methylpyrimidine and 5-(2-hydroxyethyl)-4-methylthiazole in the metabolism of thiamine (vitamin B1), in Staphylococcus aureus (Sa). SaTenA was crystallized by the vapour-diffusion method and the resulting crystal diffracted to 2.6?{\AA} resolution usng synchrotron radiation. The crystal is orthorhombic, belonging to space group P2(1)2(1)2(1) with unit-cell parameters a=103.5, b=104.1, c=109.6?{\AA}. With four molecules in the asymmetric unit, the Matthews coefficient is 2.85?{\AA}3?Da(-1). Initial attempts to solve the structure by molecular-replacement techniques were successful.",
author = "Afshan Begum and Julia Drebes and Markus Perbandt and Carsten Wrenger and Christian Betzel",
year = "2011",
language = "English",
volume = "67",
pages = "51--53",
journal = "ACTA CRYSTALLOGR F",
issn = "2053-230X",
publisher = "John Wiley and Sons Ltd",
number = "Pt 1",

}

RIS

TY - JOUR

T1 - Purification, crystallization and preliminary X-ray diffraction analysis of the thiaminase type II from Staphylococcus aureus.

AU - Begum, Afshan

AU - Drebes, Julia

AU - Perbandt, Markus

AU - Wrenger, Carsten

AU - Betzel, Christian

PY - 2011

Y1 - 2011

N2 - Thiaminase type II (TenA) catalyzes the deamination of aminopyrimidines, including the cleavage of thiamine to 4-amino-5-hydroxymethyl-2-methylpyrimidine and 5-(2-hydroxyethyl)-4-methylthiazole in the metabolism of thiamine (vitamin B1), in Staphylococcus aureus (Sa). SaTenA was crystallized by the vapour-diffusion method and the resulting crystal diffracted to 2.6?Å resolution usng synchrotron radiation. The crystal is orthorhombic, belonging to space group P2(1)2(1)2(1) with unit-cell parameters a=103.5, b=104.1, c=109.6?Å. With four molecules in the asymmetric unit, the Matthews coefficient is 2.85?Å3?Da(-1). Initial attempts to solve the structure by molecular-replacement techniques were successful.

AB - Thiaminase type II (TenA) catalyzes the deamination of aminopyrimidines, including the cleavage of thiamine to 4-amino-5-hydroxymethyl-2-methylpyrimidine and 5-(2-hydroxyethyl)-4-methylthiazole in the metabolism of thiamine (vitamin B1), in Staphylococcus aureus (Sa). SaTenA was crystallized by the vapour-diffusion method and the resulting crystal diffracted to 2.6?Å resolution usng synchrotron radiation. The crystal is orthorhombic, belonging to space group P2(1)2(1)2(1) with unit-cell parameters a=103.5, b=104.1, c=109.6?Å. With four molecules in the asymmetric unit, the Matthews coefficient is 2.85?Å3?Da(-1). Initial attempts to solve the structure by molecular-replacement techniques were successful.

M3 - SCORING: Journal article

VL - 67

SP - 51

EP - 53

JO - ACTA CRYSTALLOGR F

JF - ACTA CRYSTALLOGR F

SN - 2053-230X

IS - Pt 1

M1 - Pt 1

ER -