Purification, crystallization and preliminary X-ray diffraction analysis of crotamine, a myotoxic polypeptide from the Brazilian snake Crotalus durissus terrificus
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Purification, crystallization and preliminary X-ray diffraction analysis of crotamine, a myotoxic polypeptide from the Brazilian snake Crotalus durissus terrificus. / Coronado, Mônika A; Georgieva, Dessislava; Buck, Friedrich; Gabdoulkhakov, Azat H; Ullah, Anwar; Spencer, Patrick J; Arni, Raghuvir K; Betzel, Christian.
In: ACTA CRYSTALLOGR F, Vol. 68, No. Pt 9, 01.09.2012, p. 1052-4.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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T1 - Purification, crystallization and preliminary X-ray diffraction analysis of crotamine, a myotoxic polypeptide from the Brazilian snake Crotalus durissus terrificus
AU - Coronado, Mônika A
AU - Georgieva, Dessislava
AU - Buck, Friedrich
AU - Gabdoulkhakov, Azat H
AU - Ullah, Anwar
AU - Spencer, Patrick J
AU - Arni, Raghuvir K
AU - Betzel, Christian
PY - 2012/9/1
Y1 - 2012/9/1
N2 - Crotamine, a highly basic myotoxic polypeptide (molecular mass 4881 Da) isolated from the venom of the Brazilian rattlesnake Crotalus durissus terrificus, causes skeletal muscle contraction and spasms, affects the functioning of voltage-sensitive sodium channels by inducing sodium influx and possesses antitumour activity, suggesting potential pharmaceutical applications. Crotamine was purified from C. durissus terrificus venom; the crystals diffracted to 1.9 Å resolution and belonged to the orthorhombic space group I2(1)2(1)2(1) or I222, with unit-cell parameters a = 67.75, b = 74.4, c = 81.01 Å. The self-rotation function indicated that the asymmetric unit contained three molecules. However, structure determination by molecular replacement using NMR-determined coordinates was unsuccessful and a search for potential derivatives has been initiated.
AB - Crotamine, a highly basic myotoxic polypeptide (molecular mass 4881 Da) isolated from the venom of the Brazilian rattlesnake Crotalus durissus terrificus, causes skeletal muscle contraction and spasms, affects the functioning of voltage-sensitive sodium channels by inducing sodium influx and possesses antitumour activity, suggesting potential pharmaceutical applications. Crotamine was purified from C. durissus terrificus venom; the crystals diffracted to 1.9 Å resolution and belonged to the orthorhombic space group I2(1)2(1)2(1) or I222, with unit-cell parameters a = 67.75, b = 74.4, c = 81.01 Å. The self-rotation function indicated that the asymmetric unit contained three molecules. However, structure determination by molecular replacement using NMR-determined coordinates was unsuccessful and a search for potential derivatives has been initiated.
KW - Animals
KW - Crotalid Venoms
KW - Crotalus
KW - Crystallization
KW - Crystallography, X-Ray
U2 - 10.1107/S1744309112032721
DO - 10.1107/S1744309112032721
M3 - SCORING: Journal article
C2 - 22949192
VL - 68
SP - 1052
EP - 1054
JO - ACTA CRYSTALLOGR F
JF - ACTA CRYSTALLOGR F
SN - 2053-230X
IS - Pt 9
ER -