Proteomics: A Tool to Study Platelet Function
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Proteomics: A Tool to Study Platelet Function. / Shevchuk, Olga; Begonja, Antonija Jurak; Gambaryan, Stepan; Totzeck, Matthias; Rassaf, Tienush; Huber, Tobias B; Greinacher, Andreas; Renne, Thomas; Sickmann, Albert.
In: INT J MOL SCI, Vol. 22, No. 9, 30.04.2021, p. 4776.Research output: SCORING: Contribution to journal › SCORING: Review article › Research
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TY - JOUR
T1 - Proteomics: A Tool to Study Platelet Function
AU - Shevchuk, Olga
AU - Begonja, Antonija Jurak
AU - Gambaryan, Stepan
AU - Totzeck, Matthias
AU - Rassaf, Tienush
AU - Huber, Tobias B
AU - Greinacher, Andreas
AU - Renne, Thomas
AU - Sickmann, Albert
PY - 2021/4/30
Y1 - 2021/4/30
N2 - Platelets are components of the blood that are highly reactive, and they quickly respond to multiple physiological and pathophysiological processes. In the last decade, it became clear that platelets are the key components of circulation, linking hemostasis, innate, and acquired immunity. Protein composition, localization, and activity are crucial for platelet function and regulation. The current state of mass spectrometry-based proteomics has tremendous potential to identify and quantify thousands of proteins from a minimal amount of material, unravel multiple post-translational modifications, and monitor platelet activity during drug treatments. This review focuses on the role of proteomics in understanding the molecular basics of the classical and newly emerging functions of platelets. including the recently described role of platelets in immunology and the development of COVID-19.The state-of-the-art proteomic technologies and their application in studying platelet biogenesis, signaling, and storage are described, and the potential of newly appeared trapped ion mobility spectrometry (TIMS) is highlighted. Additionally, implementing proteomic methods in platelet transfusion medicine, and as a diagnostic and prognostic tool, is discussed.
AB - Platelets are components of the blood that are highly reactive, and they quickly respond to multiple physiological and pathophysiological processes. In the last decade, it became clear that platelets are the key components of circulation, linking hemostasis, innate, and acquired immunity. Protein composition, localization, and activity are crucial for platelet function and regulation. The current state of mass spectrometry-based proteomics has tremendous potential to identify and quantify thousands of proteins from a minimal amount of material, unravel multiple post-translational modifications, and monitor platelet activity during drug treatments. This review focuses on the role of proteomics in understanding the molecular basics of the classical and newly emerging functions of platelets. including the recently described role of platelets in immunology and the development of COVID-19.The state-of-the-art proteomic technologies and their application in studying platelet biogenesis, signaling, and storage are described, and the potential of newly appeared trapped ion mobility spectrometry (TIMS) is highlighted. Additionally, implementing proteomic methods in platelet transfusion medicine, and as a diagnostic and prognostic tool, is discussed.
KW - Animals
KW - Blood Platelets/cytology
KW - COVID-19/immunology
KW - Humans
KW - Mass Spectrometry/methods
KW - Platelet Function Tests/methods
KW - Platelet Transfusion
KW - Protein Processing, Post-Translational
KW - Proteomics/methods
KW - Signal Transduction
KW - Transfusion Medicine/methods
U2 - 10.3390/ijms22094776
DO - 10.3390/ijms22094776
M3 - SCORING: Review article
C2 - 33946341
VL - 22
SP - 4776
JO - INT J MOL SCI
JF - INT J MOL SCI
SN - 1661-6596
IS - 9
ER -
