Proteomic analysis of the rare Uracoan rattlesnake Crotalus vegrandis venom: Evidence of a broad arsenal of toxins
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Proteomic analysis of the rare Uracoan rattlesnake Crotalus vegrandis venom: Evidence of a broad arsenal of toxins. / Viala, Vincent Louis; Hildebrand, Diana; Fucase, Tamara Mieco; Sciani, Juliana Mozer; Prezotto-Neto, José Pedro; Riedner, Maria; Sanches, Leonardo; Nishimura, Paula Juliana; Oguiura, Nancy; Pimenta, Daniel Carvalho; Schlüter, Hartmut; Betzel, Christian; Arni, Raghuvir Krishnaswami; Spencer, Patrick Jack.
In: TOXICON, Vol. 107, No. Pt B, 01.12.2015, p. 234-51.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Proteomic analysis of the rare Uracoan rattlesnake Crotalus vegrandis venom: Evidence of a broad arsenal of toxins
AU - Viala, Vincent Louis
AU - Hildebrand, Diana
AU - Fucase, Tamara Mieco
AU - Sciani, Juliana Mozer
AU - Prezotto-Neto, José Pedro
AU - Riedner, Maria
AU - Sanches, Leonardo
AU - Nishimura, Paula Juliana
AU - Oguiura, Nancy
AU - Pimenta, Daniel Carvalho
AU - Schlüter, Hartmut
AU - Betzel, Christian
AU - Arni, Raghuvir Krishnaswami
AU - Spencer, Patrick Jack
N1 - Copyright © 2015 Elsevier Ltd. All rights reserved.
PY - 2015/12/1
Y1 - 2015/12/1
N2 - The investigation of venoms has many clinical, pharmacological, ecological and evolutionary outcomes. The Crotalus spp. venom can cause hemorrhage, neurotoxicity, myotoxicity, coagulopathy and hypotension. Although neurotoxicity and hemorrhage usually does not occur for the same species, the rare Venezuelan species Crotalus vegrandis presents both characteristic. Different from the other species it has a restricted ecological niche and geographical distribution. Nevertheless, it has a raising medical importance as this rattlesnake population is increasing. Few works describe its neurotoxic and hemorrhagic features, but other toxins might play an important role in envenomation. We combined proteomic methods to identify for the first time the main components of it venom: 2D SDS-PAGE and gel-filtration chromatography for protein mixture decomplexation; LC-MS(2) of low molecular mass fractions and tryptic peptides; bioinformatic identification of toxin families and specific protein species based on unique peptide analysis and sequence database enriched with species-specific venom gland transcripts; and finally polyclonal anti-crotamine Western-blotting. Our results point to a broad arsenal of toxins in C. vegrandis venom: PIII and PII metalloproteases, crotoxin subunits, other phospholipases, isoforms of serine proteases and lectins, l-amino-acid oxidase, nerve growth factor, as well as other less abundant toxins.
AB - The investigation of venoms has many clinical, pharmacological, ecological and evolutionary outcomes. The Crotalus spp. venom can cause hemorrhage, neurotoxicity, myotoxicity, coagulopathy and hypotension. Although neurotoxicity and hemorrhage usually does not occur for the same species, the rare Venezuelan species Crotalus vegrandis presents both characteristic. Different from the other species it has a restricted ecological niche and geographical distribution. Nevertheless, it has a raising medical importance as this rattlesnake population is increasing. Few works describe its neurotoxic and hemorrhagic features, but other toxins might play an important role in envenomation. We combined proteomic methods to identify for the first time the main components of it venom: 2D SDS-PAGE and gel-filtration chromatography for protein mixture decomplexation; LC-MS(2) of low molecular mass fractions and tryptic peptides; bioinformatic identification of toxin families and specific protein species based on unique peptide analysis and sequence database enriched with species-specific venom gland transcripts; and finally polyclonal anti-crotamine Western-blotting. Our results point to a broad arsenal of toxins in C. vegrandis venom: PIII and PII metalloproteases, crotoxin subunits, other phospholipases, isoforms of serine proteases and lectins, l-amino-acid oxidase, nerve growth factor, as well as other less abundant toxins.
U2 - 10.1016/j.toxicon.2015.09.023
DO - 10.1016/j.toxicon.2015.09.023
M3 - SCORING: Journal article
C2 - 26403864
VL - 107
SP - 234
EP - 251
JO - TOXICON
JF - TOXICON
SN - 0041-0101
IS - Pt B
ER -