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Proteomic Analysis in Valvular Cardiomyopathy: Aortic Regurgitation vs. Aortic Stenosis

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Proteomic Analysis in Valvular Cardiomyopathy: Aortic Regurgitation vs. Aortic Stenosis. / Holst, Theresa; Petersen, Johannes; Ameling, Sabine; Müller, Lisa; Christ, Torsten; Gedeon, Naomi; Eschenhagen, Thomas; Reichenspurner, Hermann; Hammer, Elke; Girdauskas, Evaldas.

In: CELLS-BASEL, Vol. 12, No. 6, 878, 11.03.2023.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Holst, T, Petersen, J, Ameling, S, Müller, L, Christ, T, Gedeon, N, Eschenhagen, T, Reichenspurner, H, Hammer, E & Girdauskas, E 2023, 'Proteomic Analysis in Valvular Cardiomyopathy: Aortic Regurgitation vs. Aortic Stenosis', CELLS-BASEL, vol. 12, no. 6, 878. https://doi.org/10.3390/cells12060878

APA

Holst, T., Petersen, J., Ameling, S., Müller, L., Christ, T., Gedeon, N., Eschenhagen, T., Reichenspurner, H., Hammer, E., & Girdauskas, E. (2023). Proteomic Analysis in Valvular Cardiomyopathy: Aortic Regurgitation vs. Aortic Stenosis. CELLS-BASEL, 12(6), [878]. https://doi.org/10.3390/cells12060878

Vancouver

Holst T, Petersen J, Ameling S, Müller L, Christ T, Gedeon N et al. Proteomic Analysis in Valvular Cardiomyopathy: Aortic Regurgitation vs. Aortic Stenosis. CELLS-BASEL. 2023 Mar 11;12(6). 878. https://doi.org/10.3390/cells12060878

Bibtex

@article{7108f555ca2a43b8a98749be06ea70b1,
title = "Proteomic Analysis in Valvular Cardiomyopathy: Aortic Regurgitation vs. Aortic Stenosis",
abstract = "Left ventricular (LV) reverse remodeling after aortic valve (AV) surgery is less predictable in chronic aortic regurgitation (AR) than in aortic stenosis (AS). We aimed to disclose specific LV myocardial protein signatures possibly contributing to differential disease progression. Global protein profiling of LV myocardial samples excised from the subaortic interventricular septum in patients with isolated AR or AS undergoing AV surgery was performed using liquid chromatography-electrospray ionization-tandem mass spectrometry. Based on label-free quantitation protein intensities, a logistic regression model was calculated and adjusted for age, sex and protein concentration. Web-based functional enrichment analyses of phenotype-associated proteins were performed utilizing g:Profiler and STRING. Data are available via ProteomeXchange with identifier PXD039662. Lysates from 38 patients, including 25 AR and 13 AS samples, were analyzed. AR patients presented with significantly larger LV diameters and volumes (end-diastolic diameter: 61 (12) vs. 48 (13) mm, p < 0.001; end-diastolic volume: 180.0 (74.6) vs. 92.3 (78.4), p = 0.001). A total of 171 proteins were associated with patient phenotype: 117 were positively associated with AR and the enrichment of intracellular compartment proteins (i.e., assigned to carbohydrate and nucleotide metabolism, protein biosynthesis and the proteasome) was detected. Additionally, 54 were positively associated with AS and the enrichment of extracellular compartment proteins (i.e., assigned to the immune and hematopoietic system) was observed. In summary, functional enrichment analysis revealed specific AR- and AS-associated signatures of LV myocardial proteins.",
keywords = "Humans, Aortic Valve Insufficiency/surgery, Proteomics, Aortic Valve Stenosis, Cardiomyopathies/complications, Disease Progression",
author = "Theresa Holst and Johannes Petersen and Sabine Ameling and Lisa M{\"u}ller and Torsten Christ and Naomi Gedeon and Thomas Eschenhagen and Hermann Reichenspurner and Elke Hammer and Evaldas Girdauskas",
year = "2023",
month = mar,
day = "11",
doi = "10.3390/cells12060878",
language = "English",
volume = "12",
journal = "CELLS-BASEL",
issn = "2073-4409",
publisher = "MDPI Multidisciplinary Digital Publishing Institute",
number = "6",

}

RIS

TY - JOUR

T1 - Proteomic Analysis in Valvular Cardiomyopathy: Aortic Regurgitation vs. Aortic Stenosis

AU - Holst, Theresa

AU - Petersen, Johannes

AU - Ameling, Sabine

AU - Müller, Lisa

AU - Christ, Torsten

AU - Gedeon, Naomi

AU - Eschenhagen, Thomas

AU - Reichenspurner, Hermann

AU - Hammer, Elke

AU - Girdauskas, Evaldas

PY - 2023/3/11

Y1 - 2023/3/11

N2 - Left ventricular (LV) reverse remodeling after aortic valve (AV) surgery is less predictable in chronic aortic regurgitation (AR) than in aortic stenosis (AS). We aimed to disclose specific LV myocardial protein signatures possibly contributing to differential disease progression. Global protein profiling of LV myocardial samples excised from the subaortic interventricular septum in patients with isolated AR or AS undergoing AV surgery was performed using liquid chromatography-electrospray ionization-tandem mass spectrometry. Based on label-free quantitation protein intensities, a logistic regression model was calculated and adjusted for age, sex and protein concentration. Web-based functional enrichment analyses of phenotype-associated proteins were performed utilizing g:Profiler and STRING. Data are available via ProteomeXchange with identifier PXD039662. Lysates from 38 patients, including 25 AR and 13 AS samples, were analyzed. AR patients presented with significantly larger LV diameters and volumes (end-diastolic diameter: 61 (12) vs. 48 (13) mm, p < 0.001; end-diastolic volume: 180.0 (74.6) vs. 92.3 (78.4), p = 0.001). A total of 171 proteins were associated with patient phenotype: 117 were positively associated with AR and the enrichment of intracellular compartment proteins (i.e., assigned to carbohydrate and nucleotide metabolism, protein biosynthesis and the proteasome) was detected. Additionally, 54 were positively associated with AS and the enrichment of extracellular compartment proteins (i.e., assigned to the immune and hematopoietic system) was observed. In summary, functional enrichment analysis revealed specific AR- and AS-associated signatures of LV myocardial proteins.

AB - Left ventricular (LV) reverse remodeling after aortic valve (AV) surgery is less predictable in chronic aortic regurgitation (AR) than in aortic stenosis (AS). We aimed to disclose specific LV myocardial protein signatures possibly contributing to differential disease progression. Global protein profiling of LV myocardial samples excised from the subaortic interventricular septum in patients with isolated AR or AS undergoing AV surgery was performed using liquid chromatography-electrospray ionization-tandem mass spectrometry. Based on label-free quantitation protein intensities, a logistic regression model was calculated and adjusted for age, sex and protein concentration. Web-based functional enrichment analyses of phenotype-associated proteins were performed utilizing g:Profiler and STRING. Data are available via ProteomeXchange with identifier PXD039662. Lysates from 38 patients, including 25 AR and 13 AS samples, were analyzed. AR patients presented with significantly larger LV diameters and volumes (end-diastolic diameter: 61 (12) vs. 48 (13) mm, p < 0.001; end-diastolic volume: 180.0 (74.6) vs. 92.3 (78.4), p = 0.001). A total of 171 proteins were associated with patient phenotype: 117 were positively associated with AR and the enrichment of intracellular compartment proteins (i.e., assigned to carbohydrate and nucleotide metabolism, protein biosynthesis and the proteasome) was detected. Additionally, 54 were positively associated with AS and the enrichment of extracellular compartment proteins (i.e., assigned to the immune and hematopoietic system) was observed. In summary, functional enrichment analysis revealed specific AR- and AS-associated signatures of LV myocardial proteins.

KW - Humans

KW - Aortic Valve Insufficiency/surgery

KW - Proteomics

KW - Aortic Valve Stenosis

KW - Cardiomyopathies/complications

KW - Disease Progression

U2 - 10.3390/cells12060878

DO - 10.3390/cells12060878

M3 - SCORING: Journal article

C2 - 36980219

VL - 12

JO - CELLS-BASEL

JF - CELLS-BASEL

SN - 2073-4409

IS - 6

M1 - 878

ER -