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Proof of principle

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Proof of principle : detection of genotoxicity by a fluorescence-based recombination test in mammalian cells. / Akyüz, Nuray; Wiesmüller, Lisa.

In: ALTEX-ALTERN ANIM EX, Vol. 20, No. 2, 2003, p. 77-84.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Akyüz, N & Wiesmüller, L 2003, 'Proof of principle: detection of genotoxicity by a fluorescence-based recombination test in mammalian cells', ALTEX-ALTERN ANIM EX, vol. 20, no. 2, pp. 77-84.

APA

Akyüz, N., & Wiesmüller, L. (2003). Proof of principle: detection of genotoxicity by a fluorescence-based recombination test in mammalian cells. ALTEX-ALTERN ANIM EX, 20(2), 77-84.

Vancouver

Akyüz N, Wiesmüller L. Proof of principle: detection of genotoxicity by a fluorescence-based recombination test in mammalian cells. ALTEX-ALTERN ANIM EX. 2003;20(2):77-84.

Bibtex

@article{8251068c84be471899e91f52461f6259,
title = "Proof of principle: detection of genotoxicity by a fluorescence-based recombination test in mammalian cells",
abstract = "Genotoxicity tests available today have several shortcomings. The widely applied Ames assay measures mutations in bacteria, thereby disregarding the physiological particularities of the human cell and organism.We provide first evidence for a new concept of genotoxicity detection in living human cell cultures. The data were obtained by use of a newly developed assay, which is based on the quantification of fluorescent signals, i.e. counting of the relative number of fluorescent cells in the sample. It is characterised by a short reaction time and fulfills the requirements for automated performance. The new system monitors chromosomal rearrangements and, therefore, is predicted to detect a broad spectrum of genotoxic substances. Indeed, we demonstrate the genotoxic effect of ionising radiation, of an Ames assay positive compound, and of two compounds which are poorly mutagenic in the Ames assay. The new assay will be optimised further and adapted to the requirements for routine analysis in order to help to further reduce animal experimentation in genotoxicity testing.",
keywords = "Animal Testing Alternatives, Animals, Cell Line, DNA Damage, Etoposide, Green Fluorescent Proteins, Humans, Luminescent Proteins, Mutagens, Recombinant Proteins, Recombination, Genetic, Spectrometry, Fluorescence, Tumor Cells, Cultured",
author = "Nuray Aky{\"u}z and Lisa Wiesm{\"u}ller",
year = "2003",
language = "English",
volume = "20",
pages = "77--84",
journal = "ALTEX-ALTERN ANIM EX",
issn = "1868-596X",
publisher = "Springer International Publishing",
number = "2",

}

RIS

TY - JOUR

T1 - Proof of principle

T2 - detection of genotoxicity by a fluorescence-based recombination test in mammalian cells

AU - Akyüz, Nuray

AU - Wiesmüller, Lisa

PY - 2003

Y1 - 2003

N2 - Genotoxicity tests available today have several shortcomings. The widely applied Ames assay measures mutations in bacteria, thereby disregarding the physiological particularities of the human cell and organism.We provide first evidence for a new concept of genotoxicity detection in living human cell cultures. The data were obtained by use of a newly developed assay, which is based on the quantification of fluorescent signals, i.e. counting of the relative number of fluorescent cells in the sample. It is characterised by a short reaction time and fulfills the requirements for automated performance. The new system monitors chromosomal rearrangements and, therefore, is predicted to detect a broad spectrum of genotoxic substances. Indeed, we demonstrate the genotoxic effect of ionising radiation, of an Ames assay positive compound, and of two compounds which are poorly mutagenic in the Ames assay. The new assay will be optimised further and adapted to the requirements for routine analysis in order to help to further reduce animal experimentation in genotoxicity testing.

AB - Genotoxicity tests available today have several shortcomings. The widely applied Ames assay measures mutations in bacteria, thereby disregarding the physiological particularities of the human cell and organism.We provide first evidence for a new concept of genotoxicity detection in living human cell cultures. The data were obtained by use of a newly developed assay, which is based on the quantification of fluorescent signals, i.e. counting of the relative number of fluorescent cells in the sample. It is characterised by a short reaction time and fulfills the requirements for automated performance. The new system monitors chromosomal rearrangements and, therefore, is predicted to detect a broad spectrum of genotoxic substances. Indeed, we demonstrate the genotoxic effect of ionising radiation, of an Ames assay positive compound, and of two compounds which are poorly mutagenic in the Ames assay. The new assay will be optimised further and adapted to the requirements for routine analysis in order to help to further reduce animal experimentation in genotoxicity testing.

KW - Animal Testing Alternatives

KW - Animals

KW - Cell Line

KW - DNA Damage

KW - Etoposide

KW - Green Fluorescent Proteins

KW - Humans

KW - Luminescent Proteins

KW - Mutagens

KW - Recombinant Proteins

KW - Recombination, Genetic

KW - Spectrometry, Fluorescence

KW - Tumor Cells, Cultured

M3 - SCORING: Journal article

C2 - 12764544

VL - 20

SP - 77

EP - 84

JO - ALTEX-ALTERN ANIM EX

JF - ALTEX-ALTERN ANIM EX

SN - 1868-596X

IS - 2

ER -