Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer
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Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer : Sci Rep. / Seitz, A. K.; Christensen, L. L.; Christensen, E.; Faarkrog, K.; Ostenfeld, M. S.; Hedegaard, J.; Nordentoft, I.; Nielsen, M. M.; Palmfeldt, J.; Thomson, M.; Jensen, M. T.; Nawroth, R.; Maurer, T.; Orntoft, T. F.; Jensen, J. B.; Damgaard, C. K.; Dyrskjot, L.
In: SCI REP-UK, Vol. 7, No. 1, 2017, p. 395.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research
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TY - JOUR
T1 - Profiling of long non-coding RNAs identifies LINC00958 and LINC01296 as candidate oncogenes in bladder cancer
T2 - Sci Rep
AU - Seitz, A. K.
AU - Christensen, L. L.
AU - Christensen, E.
AU - Faarkrog, K.
AU - Ostenfeld, M. S.
AU - Hedegaard, J.
AU - Nordentoft, I.
AU - Nielsen, M. M.
AU - Palmfeldt, J.
AU - Thomson, M.
AU - Jensen, M. T.
AU - Nawroth, R.
AU - Maurer, T.
AU - Orntoft, T. F.
AU - Jensen, J. B.
AU - Damgaard, C. K.
AU - Dyrskjot, L.
N1 - 2045-2322 Seitz, Anna Katharina Christensen, Lise Lotte Christensen, Emil Faarkrog, Kasper Ostenfeld, Marie Stampe Hedegaard, Jakob Nordentoft, Iver Nielsen, Morten Muhlig Palmfeldt, Johan Thomson, Michelle Jensen, Michael Theis Solgaard Nawroth, Roman Maurer, Tobias Orntoft, Torben Falck Jensen, Jorgen Bjerggaard Damgaard, Christian Kroun Dyrskjot, Lars Journal Article Research Support, Non-U.S. Gov't England Sci Rep. 2017 Mar 24;7(1):395. doi: 10.1038/s41598-017-00327-0.
PY - 2017
Y1 - 2017
N2 - Aberrant expression of long non-coding RNAs (lncRNAs) has been regarded as a critical component in bladder cancer (BC) and lncRNAs have been associated with BC development and progression although their overall expression and functional significance is still unclear. The aim of our study was to identify novel lncRNAs with a functional role in BC carcinogenesis. RNA-sequencing was used to identify aberrantly expressed lncRNAs in 8 normal and 72 BC samples. We identified 89 lncRNAs that were significantly dys-regulated in BC. Five lncRNAs; LINC00958, LINC01296, LINC00355, LNC-CMC1-1 and LNC-ALX1-2 were selected for further analyses. Silencing of LINC00958 or LINC01296 in vitro reduced both cell viability and migration. Knock-down of LINC00958 also affected invasion and resistance to anoikis. These cellular effects could be linked to direct/indirect regulation of protein coding mRNAs involved in cell death/survival, proliferation and cellular movement. Finally, we showed that LINC00958 binds proteins involved in regulation and initiation of translation and in post-transcriptional modification of RNA, including Metadherin, which has previously been associated with BC. Our analyses identified novel lncRNAs in BC that likely act as oncogenic drivers contributing to an aggressive cancerous phenotype likely through interaction with proteins involved in initiation of translation and/or post-transcriptional modification of RNA.
AB - Aberrant expression of long non-coding RNAs (lncRNAs) has been regarded as a critical component in bladder cancer (BC) and lncRNAs have been associated with BC development and progression although their overall expression and functional significance is still unclear. The aim of our study was to identify novel lncRNAs with a functional role in BC carcinogenesis. RNA-sequencing was used to identify aberrantly expressed lncRNAs in 8 normal and 72 BC samples. We identified 89 lncRNAs that were significantly dys-regulated in BC. Five lncRNAs; LINC00958, LINC01296, LINC00355, LNC-CMC1-1 and LNC-ALX1-2 were selected for further analyses. Silencing of LINC00958 or LINC01296 in vitro reduced both cell viability and migration. Knock-down of LINC00958 also affected invasion and resistance to anoikis. These cellular effects could be linked to direct/indirect regulation of protein coding mRNAs involved in cell death/survival, proliferation and cellular movement. Finally, we showed that LINC00958 binds proteins involved in regulation and initiation of translation and in post-transcriptional modification of RNA, including Metadherin, which has previously been associated with BC. Our analyses identified novel lncRNAs in BC that likely act as oncogenic drivers contributing to an aggressive cancerous phenotype likely through interaction with proteins involved in initiation of translation and/or post-transcriptional modification of RNA.
KW - Cell Line, Tumor Cell Survival Gene Expression Profiling Gene Expression Regulation, Neoplastic Humans Oncogenes RNA, Long Noncoding/genetics Transcriptome Up-Regulation Urinary Bladder Neoplasms/genetics
U2 - 10.1038/s41598-017-00327-0
DO - 10.1038/s41598-017-00327-0
M3 - SCORING: Journal article
VL - 7
SP - 395
JO - SCI REP-UK
JF - SCI REP-UK
SN - 2045-2322
IS - 1
ER -
