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Prevalence of proliferating CD8+ cells in normal lymphatic tissues, inflammation and cancer

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Prevalence of proliferating CD8+ cells in normal lymphatic tissues, inflammation and cancer. / Blessin, Niclas C; Abu-Hashem, Raed; Mandelkow, Tim; Li, Wenchao; Simon, Ronald; Hube-Magg, Claudia; Möller-Koop, Christina; Witt, Melanie; Schmidt, Alice; Büscheck, Franziska; Fraune, Christoph; Luebke, Andreas M; Möller, Katharina; Jacobsen, Frank; Lutz, Florian; Lennartz, Maximilian; Steurer, Stefan; Sauter, Guido; Höflmayer, Doris; Tsourlakis, Maria Christina; Hinsch, Andrea; Burandt, Eike; Wilczak, Waldemar; Minner, Sarah; Clauditz, Till S.

In: AGING-US, Vol. 13, No. 11, 03.06.2021, p. 14590-14603.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Blessin, NC, Abu-Hashem, R, Mandelkow, T, Li, W, Simon, R, Hube-Magg, C, Möller-Koop, C, Witt, M, Schmidt, A, Büscheck, F, Fraune, C, Luebke, AM, Möller, K, Jacobsen, F, Lutz, F, Lennartz, M, Steurer, S, Sauter, G, Höflmayer, D, Tsourlakis, MC, Hinsch, A, Burandt, E, Wilczak, W, Minner, S & Clauditz, TS 2021, 'Prevalence of proliferating CD8+ cells in normal lymphatic tissues, inflammation and cancer', AGING-US, vol. 13, no. 11, pp. 14590-14603. https://doi.org/10.18632/aging.203113

APA

Blessin, N. C., Abu-Hashem, R., Mandelkow, T., Li, W., Simon, R., Hube-Magg, C., Möller-Koop, C., Witt, M., Schmidt, A., Büscheck, F., Fraune, C., Luebke, A. M., Möller, K., Jacobsen, F., Lutz, F., Lennartz, M., Steurer, S., Sauter, G., Höflmayer, D., ... Clauditz, T. S. (2021). Prevalence of proliferating CD8+ cells in normal lymphatic tissues, inflammation and cancer. AGING-US, 13(11), 14590-14603. https://doi.org/10.18632/aging.203113

Vancouver

Blessin NC, Abu-Hashem R, Mandelkow T, Li W, Simon R, Hube-Magg C et al. Prevalence of proliferating CD8+ cells in normal lymphatic tissues, inflammation and cancer. AGING-US. 2021 Jun 3;13(11):14590-14603. https://doi.org/10.18632/aging.203113

Bibtex

@article{c63596b4da154dbcb6dd6fe3351b8906,
title = "Prevalence of proliferating CD8+ cells in normal lymphatic tissues, inflammation and cancer",
abstract = "CD8+ cytotoxic T-lymphocytes are essential components of the anti-tumor immunity. To better understand the expansion of CD8+ T-cells we used multiplex fluorescence immunohistochemistry to study Ki67+CD8+ cells in normal lymphoid tissues, selected inflammatory diseases and cancers in 41 large sections/ microenvironment tissue microarrays (TMAs) as well as 765 samples in a conventional TMA format. The evaluation of more than 20 different compartments of normal lymphoid tissues revealed that the percentage of proliferating (ki67+) CD8+ cells did commonly not exceed 3%. In inflammations, the percentage of Ki67+CD8+ cells was more variable and higher compared to normal tissues. In cancers, the percentage of Ki67+CD8+ cells was higher in the tumor center than at the invasive margin. In the tumor center of 765 colorectal cancers, the density of Ki67+CD8+ cells and the percentage of proliferating CD8+ cytotoxic T-cells was significantly associated with microsatellite instability (p<0.0001), pT (p<0.0002) and pN category (p<0.0098). In summary, these data show that the percentage of Ki67+CD8+ cells is usually at a baseline proliferation rate below 3% in healthy secondary lymphoid organs. This rate is often markedly higher in inflammatory and neoplastic diseases compared to normal tissues. The striking link with unfavorable tumor features in colorectal cancer suggest a potential clinical utility of assessing the percentage of Ki67+CD8+ cells to predict patients outcome.",
keywords = "CD8-Positive T-Lymphocytes/immunology, Cell Proliferation, Colorectal Neoplasms/immunology, Humans, Inflammation/immunology, Ki-67 Antigen/metabolism, Lymphoid Tissue/immunology, Phenotype",
author = "Blessin, {Niclas C} and Raed Abu-Hashem and Tim Mandelkow and Wenchao Li and Ronald Simon and Claudia Hube-Magg and Christina M{\"o}ller-Koop and Melanie Witt and Alice Schmidt and Franziska B{\"u}scheck and Christoph Fraune and Luebke, {Andreas M} and Katharina M{\"o}ller and Frank Jacobsen and Florian Lutz and Maximilian Lennartz and Stefan Steurer and Guido Sauter and Doris H{\"o}flmayer and Tsourlakis, {Maria Christina} and Andrea Hinsch and Eike Burandt and Waldemar Wilczak and Sarah Minner and Clauditz, {Till S}",
year = "2021",
month = jun,
day = "3",
doi = "10.18632/aging.203113",
language = "English",
volume = "13",
pages = "14590--14603",
journal = "AGING-US",
issn = "1945-4589",
publisher = "US Administration on Aging",
number = "11",

}

RIS

TY - JOUR

T1 - Prevalence of proliferating CD8+ cells in normal lymphatic tissues, inflammation and cancer

AU - Blessin, Niclas C

AU - Abu-Hashem, Raed

AU - Mandelkow, Tim

AU - Li, Wenchao

AU - Simon, Ronald

AU - Hube-Magg, Claudia

AU - Möller-Koop, Christina

AU - Witt, Melanie

AU - Schmidt, Alice

AU - Büscheck, Franziska

AU - Fraune, Christoph

AU - Luebke, Andreas M

AU - Möller, Katharina

AU - Jacobsen, Frank

AU - Lutz, Florian

AU - Lennartz, Maximilian

AU - Steurer, Stefan

AU - Sauter, Guido

AU - Höflmayer, Doris

AU - Tsourlakis, Maria Christina

AU - Hinsch, Andrea

AU - Burandt, Eike

AU - Wilczak, Waldemar

AU - Minner, Sarah

AU - Clauditz, Till S

PY - 2021/6/3

Y1 - 2021/6/3

N2 - CD8+ cytotoxic T-lymphocytes are essential components of the anti-tumor immunity. To better understand the expansion of CD8+ T-cells we used multiplex fluorescence immunohistochemistry to study Ki67+CD8+ cells in normal lymphoid tissues, selected inflammatory diseases and cancers in 41 large sections/ microenvironment tissue microarrays (TMAs) as well as 765 samples in a conventional TMA format. The evaluation of more than 20 different compartments of normal lymphoid tissues revealed that the percentage of proliferating (ki67+) CD8+ cells did commonly not exceed 3%. In inflammations, the percentage of Ki67+CD8+ cells was more variable and higher compared to normal tissues. In cancers, the percentage of Ki67+CD8+ cells was higher in the tumor center than at the invasive margin. In the tumor center of 765 colorectal cancers, the density of Ki67+CD8+ cells and the percentage of proliferating CD8+ cytotoxic T-cells was significantly associated with microsatellite instability (p<0.0001), pT (p<0.0002) and pN category (p<0.0098). In summary, these data show that the percentage of Ki67+CD8+ cells is usually at a baseline proliferation rate below 3% in healthy secondary lymphoid organs. This rate is often markedly higher in inflammatory and neoplastic diseases compared to normal tissues. The striking link with unfavorable tumor features in colorectal cancer suggest a potential clinical utility of assessing the percentage of Ki67+CD8+ cells to predict patients outcome.

AB - CD8+ cytotoxic T-lymphocytes are essential components of the anti-tumor immunity. To better understand the expansion of CD8+ T-cells we used multiplex fluorescence immunohistochemistry to study Ki67+CD8+ cells in normal lymphoid tissues, selected inflammatory diseases and cancers in 41 large sections/ microenvironment tissue microarrays (TMAs) as well as 765 samples in a conventional TMA format. The evaluation of more than 20 different compartments of normal lymphoid tissues revealed that the percentage of proliferating (ki67+) CD8+ cells did commonly not exceed 3%. In inflammations, the percentage of Ki67+CD8+ cells was more variable and higher compared to normal tissues. In cancers, the percentage of Ki67+CD8+ cells was higher in the tumor center than at the invasive margin. In the tumor center of 765 colorectal cancers, the density of Ki67+CD8+ cells and the percentage of proliferating CD8+ cytotoxic T-cells was significantly associated with microsatellite instability (p<0.0001), pT (p<0.0002) and pN category (p<0.0098). In summary, these data show that the percentage of Ki67+CD8+ cells is usually at a baseline proliferation rate below 3% in healthy secondary lymphoid organs. This rate is often markedly higher in inflammatory and neoplastic diseases compared to normal tissues. The striking link with unfavorable tumor features in colorectal cancer suggest a potential clinical utility of assessing the percentage of Ki67+CD8+ cells to predict patients outcome.

KW - CD8-Positive T-Lymphocytes/immunology

KW - Cell Proliferation

KW - Colorectal Neoplasms/immunology

KW - Humans

KW - Inflammation/immunology

KW - Ki-67 Antigen/metabolism

KW - Lymphoid Tissue/immunology

KW - Phenotype

U2 - 10.18632/aging.203113

DO - 10.18632/aging.203113

M3 - SCORING: Journal article

C2 - 34083496

VL - 13

SP - 14590

EP - 14603

JO - AGING-US

JF - AGING-US

SN - 1945-4589

IS - 11

ER -