Preliminary crystallographic analysis of a cruciferin protein from seeds of Moringa oleifera
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Preliminary crystallographic analysis of a cruciferin protein from seeds of Moringa oleifera. / Akrem, Ahmed; Yousef, Nasser; Begum, Afshan; Negm, Amr; Meyer, Arne; Perbandt, Markus; Buck, Friedrich; Betzel, Christian.
In: PROTEIN J, Vol. 33, No. 3, 01.06.2014, p. 253-7.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Preliminary crystallographic analysis of a cruciferin protein from seeds of Moringa oleifera
AU - Akrem, Ahmed
AU - Yousef, Nasser
AU - Begum, Afshan
AU - Negm, Amr
AU - Meyer, Arne
AU - Perbandt, Markus
AU - Buck, Friedrich
AU - Betzel, Christian
PY - 2014/6/1
Y1 - 2014/6/1
N2 - A 55 kDa cruciferin protein has been purified and characterized from seeds of Moringa oleifera plant. Protein blast of N-terminal amino-acid sequence showed 60 % sequence similarity with cruciferin from Brassica napus. The M. oleifera protein has been crystallized applying the sitting drop method using 5 % polyethylene glycol 8,000, 38.5 % 3-methyl-1,5-pentanediol and 0.1 M sodium cacodylate pH 6.5. The crystals belonged to the P6322 hexagonal space group with cell dimensions, a = b = 98.4, c = 274.3 Å. Initial diffraction data have been collected to a resolution of 6 Å.
AB - A 55 kDa cruciferin protein has been purified and characterized from seeds of Moringa oleifera plant. Protein blast of N-terminal amino-acid sequence showed 60 % sequence similarity with cruciferin from Brassica napus. The M. oleifera protein has been crystallized applying the sitting drop method using 5 % polyethylene glycol 8,000, 38.5 % 3-methyl-1,5-pentanediol and 0.1 M sodium cacodylate pH 6.5. The crystals belonged to the P6322 hexagonal space group with cell dimensions, a = b = 98.4, c = 274.3 Å. Initial diffraction data have been collected to a resolution of 6 Å.
U2 - 10.1007/s10930-014-9558-x
DO - 10.1007/s10930-014-9558-x
M3 - SCORING: Journal article
C2 - 24705831
VL - 33
SP - 253
EP - 257
JO - PROTEIN J
JF - PROTEIN J
SN - 1572-3887
IS - 3
ER -