Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study

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Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study. / Popal, Zohal; Nickel, Katrin F; Wöltje, Michael; Aibibu, Dilbar; Knipfer, Christian; Smeets, Ralf; Renné, Thomas.

In: INT J IMPLANT DENT, Vol. 9, No. 1, 17.11.2023, p. 44.

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@article{398d2826240c4c93ace666ea680abcdd,
title = "Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study",
abstract = "PURPOSE: Post-interventional hemorrhage can result in serious complications, especially in patients with hemostatic disorders. Identification of safe and efficient local hemostatic agents is important, particularly in the context of an ageing society and the emergence of new oral anticoagulants. The aim of this in vitro study was to investigate the potential of silk fibroin membranes coated with the inorganic polymer polyphosphate (polyP) as a novel hemostatic device in oral surgery.METHODS: Cocoons of the silkworm Bombyx mori were degummed and dissolved. Varying amounts of long-chain polyP (2-2000 µg/mm2) were adsorbed to the surface of silk fibroin membranes. Analysis of the procoagulant effect of polyP-coated silk membranes was performed using real-time thrombin generation assays in human plasma. Increasing concentrations of polyP (0.15-500 µg/ml) served as a positive control, while uncoated silk fibroin membranes were used as negative control.RESULTS: PolyP-coated silk fibroin membranes triggered coagulation when compared to plasma samples and pure silk fibroin membranes. A polyP-dose-dependent effect of thrombin generation could be found with a maximum (ETP = 1525.7 nM⋅min, peak thrombin = 310.1 nM, time to peak = 9.8 min, lag time = 7.6 min.) at 200 µg/mm2 of polymer loading on the silk fibroin membrane surface.CONCLUSIONS: In this study, it was demonstrated that silk fibroin membranes coated with polyP have the potential to act as a promising novel hemostatic device.",
author = "Zohal Popal and Nickel, {Katrin F} and Michael W{\"o}ltje and Dilbar Aibibu and Christian Knipfer and Ralf Smeets and Thomas Renn{\'e}",
note = "{\textcopyright} 2023. The Author(s).",
year = "2023",
month = nov,
day = "17",
doi = "10.1186/s40729-023-00503-0",
language = "English",
volume = "9",
pages = "44",
journal = "INT J IMPLANT DENT",
issn = "2198-4034",
publisher = "Springer",
number = "1",

}

RIS

TY - JOUR

T1 - Polyphosphate-loaded silk fibroin membrane as hemostatic agent in oral surgery: a pilot study

AU - Popal, Zohal

AU - Nickel, Katrin F

AU - Wöltje, Michael

AU - Aibibu, Dilbar

AU - Knipfer, Christian

AU - Smeets, Ralf

AU - Renné, Thomas

N1 - © 2023. The Author(s).

PY - 2023/11/17

Y1 - 2023/11/17

N2 - PURPOSE: Post-interventional hemorrhage can result in serious complications, especially in patients with hemostatic disorders. Identification of safe and efficient local hemostatic agents is important, particularly in the context of an ageing society and the emergence of new oral anticoagulants. The aim of this in vitro study was to investigate the potential of silk fibroin membranes coated with the inorganic polymer polyphosphate (polyP) as a novel hemostatic device in oral surgery.METHODS: Cocoons of the silkworm Bombyx mori were degummed and dissolved. Varying amounts of long-chain polyP (2-2000 µg/mm2) were adsorbed to the surface of silk fibroin membranes. Analysis of the procoagulant effect of polyP-coated silk membranes was performed using real-time thrombin generation assays in human plasma. Increasing concentrations of polyP (0.15-500 µg/ml) served as a positive control, while uncoated silk fibroin membranes were used as negative control.RESULTS: PolyP-coated silk fibroin membranes triggered coagulation when compared to plasma samples and pure silk fibroin membranes. A polyP-dose-dependent effect of thrombin generation could be found with a maximum (ETP = 1525.7 nM⋅min, peak thrombin = 310.1 nM, time to peak = 9.8 min, lag time = 7.6 min.) at 200 µg/mm2 of polymer loading on the silk fibroin membrane surface.CONCLUSIONS: In this study, it was demonstrated that silk fibroin membranes coated with polyP have the potential to act as a promising novel hemostatic device.

AB - PURPOSE: Post-interventional hemorrhage can result in serious complications, especially in patients with hemostatic disorders. Identification of safe and efficient local hemostatic agents is important, particularly in the context of an ageing society and the emergence of new oral anticoagulants. The aim of this in vitro study was to investigate the potential of silk fibroin membranes coated with the inorganic polymer polyphosphate (polyP) as a novel hemostatic device in oral surgery.METHODS: Cocoons of the silkworm Bombyx mori were degummed and dissolved. Varying amounts of long-chain polyP (2-2000 µg/mm2) were adsorbed to the surface of silk fibroin membranes. Analysis of the procoagulant effect of polyP-coated silk membranes was performed using real-time thrombin generation assays in human plasma. Increasing concentrations of polyP (0.15-500 µg/ml) served as a positive control, while uncoated silk fibroin membranes were used as negative control.RESULTS: PolyP-coated silk fibroin membranes triggered coagulation when compared to plasma samples and pure silk fibroin membranes. A polyP-dose-dependent effect of thrombin generation could be found with a maximum (ETP = 1525.7 nM⋅min, peak thrombin = 310.1 nM, time to peak = 9.8 min, lag time = 7.6 min.) at 200 µg/mm2 of polymer loading on the silk fibroin membrane surface.CONCLUSIONS: In this study, it was demonstrated that silk fibroin membranes coated with polyP have the potential to act as a promising novel hemostatic device.

U2 - 10.1186/s40729-023-00503-0

DO - 10.1186/s40729-023-00503-0

M3 - SCORING: Journal article

C2 - 37975954

VL - 9

SP - 44

JO - INT J IMPLANT DENT

JF - INT J IMPLANT DENT

SN - 2198-4034

IS - 1

ER -