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Podoplanin increases migration and angiogenesis in malignant glioma

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Podoplanin increases migration and angiogenesis in malignant glioma. / Grau, Stefan J; Trillsch, Fabian; Tonn, Joerg-Christian; Goldbrunner, Roland H; Noessner, Elfriede; Nelson, Peter J; von Luettichau, Irene.

In: AM J CLIN PATHOL, Vol. 8, No. 7, 2015, p. 8663-70.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Grau, SJ, Trillsch, F, Tonn, J-C, Goldbrunner, RH, Noessner, E, Nelson, PJ & von Luettichau, I 2015, 'Podoplanin increases migration and angiogenesis in malignant glioma', AM J CLIN PATHOL, vol. 8, no. 7, pp. 8663-70.

APA

Grau, S. J., Trillsch, F., Tonn, J-C., Goldbrunner, R. H., Noessner, E., Nelson, P. J., & von Luettichau, I. (2015). Podoplanin increases migration and angiogenesis in malignant glioma. AM J CLIN PATHOL, 8(7), 8663-70.

Vancouver

Grau SJ, Trillsch F, Tonn J-C, Goldbrunner RH, Noessner E, Nelson PJ et al. Podoplanin increases migration and angiogenesis in malignant glioma. AM J CLIN PATHOL. 2015;8(7):8663-70.

Bibtex

@article{54c4a1bfb4934c789a1cad33993e6e92,
title = "Podoplanin increases migration and angiogenesis in malignant glioma",
abstract = "Expression of podoplanin in glial brain tumors is grade dependent. While serving as a marker for tumor progression and modulating invasion in various neoplasms, little is known about podoplanin function in gliomas. Therefore we stably transfected two human glioma cell lines (U373MG and U87MG) with expression plasmids encoding podoplanin. The efficacy of transfection was confirmed by FACS analysis, PCR and immunocytochemistry. Cells were then sorted for highly podoplanin expressing cells (U373P(high)/U87P(high)). Transfection did not influence the production of pro-angiogenic factors including VEGF, VEGF-C and D. Also, expression of VEGF receptors (VEGFR) remained unchanged except for U87P(high), where a VEGFR3 expression was induced. U373P(high) showed significantly reduced proliferation as compared to mock transfected group. By contrast, podoplanin significantly increased migration and invasion into collagen matrix. Furthermore, conditioned media from P(high) glioma cells strongly induced tube formation on matrigel. In conclusion, podoplanin increased migration of tumor cells and enhanced tube formation activity in endothelial cells independent from VEGF. Thus, podoplanin expression may be an important step in tumor progression.",
author = "Grau, {Stefan J} and Fabian Trillsch and Joerg-Christian Tonn and Goldbrunner, {Roland H} and Elfriede Noessner and Nelson, {Peter J} and {von Luettichau}, Irene",
year = "2015",
language = "English",
volume = "8",
pages = "8663--70",
journal = "AM J CLIN PATHOL",
issn = "0002-9173",
publisher = "American Society of Clinical Pathologists",
number = "7",

}

RIS

TY - JOUR

T1 - Podoplanin increases migration and angiogenesis in malignant glioma

AU - Grau, Stefan J

AU - Trillsch, Fabian

AU - Tonn, Joerg-Christian

AU - Goldbrunner, Roland H

AU - Noessner, Elfriede

AU - Nelson, Peter J

AU - von Luettichau, Irene

PY - 2015

Y1 - 2015

N2 - Expression of podoplanin in glial brain tumors is grade dependent. While serving as a marker for tumor progression and modulating invasion in various neoplasms, little is known about podoplanin function in gliomas. Therefore we stably transfected two human glioma cell lines (U373MG and U87MG) with expression plasmids encoding podoplanin. The efficacy of transfection was confirmed by FACS analysis, PCR and immunocytochemistry. Cells were then sorted for highly podoplanin expressing cells (U373P(high)/U87P(high)). Transfection did not influence the production of pro-angiogenic factors including VEGF, VEGF-C and D. Also, expression of VEGF receptors (VEGFR) remained unchanged except for U87P(high), where a VEGFR3 expression was induced. U373P(high) showed significantly reduced proliferation as compared to mock transfected group. By contrast, podoplanin significantly increased migration and invasion into collagen matrix. Furthermore, conditioned media from P(high) glioma cells strongly induced tube formation on matrigel. In conclusion, podoplanin increased migration of tumor cells and enhanced tube formation activity in endothelial cells independent from VEGF. Thus, podoplanin expression may be an important step in tumor progression.

AB - Expression of podoplanin in glial brain tumors is grade dependent. While serving as a marker for tumor progression and modulating invasion in various neoplasms, little is known about podoplanin function in gliomas. Therefore we stably transfected two human glioma cell lines (U373MG and U87MG) with expression plasmids encoding podoplanin. The efficacy of transfection was confirmed by FACS analysis, PCR and immunocytochemistry. Cells were then sorted for highly podoplanin expressing cells (U373P(high)/U87P(high)). Transfection did not influence the production of pro-angiogenic factors including VEGF, VEGF-C and D. Also, expression of VEGF receptors (VEGFR) remained unchanged except for U87P(high), where a VEGFR3 expression was induced. U373P(high) showed significantly reduced proliferation as compared to mock transfected group. By contrast, podoplanin significantly increased migration and invasion into collagen matrix. Furthermore, conditioned media from P(high) glioma cells strongly induced tube formation on matrigel. In conclusion, podoplanin increased migration of tumor cells and enhanced tube formation activity in endothelial cells independent from VEGF. Thus, podoplanin expression may be an important step in tumor progression.

M3 - SCORING: Journal article

C2 - 26339454

VL - 8

SP - 8663

EP - 8670

JO - AM J CLIN PATHOL

JF - AM J CLIN PATHOL

SN - 0002-9173

IS - 7

ER -