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Podocyte migration during nephrotic syndrome requires a coordinated interplay between cathepsin L and alpha3 integrin

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Podocyte migration during nephrotic syndrome requires a coordinated interplay between cathepsin L and alpha3 integrin. / Reiser, Jochen; Oh, Jun; Shirato, Isao; Asanuma, Katsuhiko; Hug, Andreas; Mundel, Thomas M; Honey, Karen; Ishidoh, Kazumi; Kominami, Eiki; Kreidberg, Jordan A; Tomino, Yasuhiko; Mundel, Peter.

In: J BIOL CHEM, Vol. 279, No. 33, 13.08.2004, p. 34827-32.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Reiser, J, Oh, J, Shirato, I, Asanuma, K, Hug, A, Mundel, TM, Honey, K, Ishidoh, K, Kominami, E, Kreidberg, JA, Tomino, Y & Mundel, P 2004, 'Podocyte migration during nephrotic syndrome requires a coordinated interplay between cathepsin L and alpha3 integrin', J BIOL CHEM, vol. 279, no. 33, pp. 34827-32. https://doi.org/10.1074/jbc.M401973200

APA

Reiser, J., Oh, J., Shirato, I., Asanuma, K., Hug, A., Mundel, T. M., Honey, K., Ishidoh, K., Kominami, E., Kreidberg, J. A., Tomino, Y., & Mundel, P. (2004). Podocyte migration during nephrotic syndrome requires a coordinated interplay between cathepsin L and alpha3 integrin. J BIOL CHEM, 279(33), 34827-32. https://doi.org/10.1074/jbc.M401973200

Vancouver

Reiser J, Oh J, Shirato I, Asanuma K, Hug A, Mundel TM et al. Podocyte migration during nephrotic syndrome requires a coordinated interplay between cathepsin L and alpha3 integrin. J BIOL CHEM. 2004 Aug 13;279(33):34827-32. https://doi.org/10.1074/jbc.M401973200

Bibtex

@article{7e6d05a98dc7458cba061ad8d64a46a0,
title = "Podocyte migration during nephrotic syndrome requires a coordinated interplay between cathepsin L and alpha3 integrin",
abstract = "Podocyte foot process effacement and disruption of the slit diaphragm are typically associated with glomerular proteinuria and can be induced in rats by the injection of puromycin aminonucleoside. Here, we show that the induction of puromycin aminonucleoside nephrosis involves podocyte migration conducted by a coordinated interplay between the cysteine protease cathepsin L and alpha(3) integrin. Puromycin aminonucleoside treatment up-regulates cathepsin L expression in podocytes in vivo as well as expression and enzymatic activity of cathepsin L in podocytes in vitro. Isolated podocytes from mice lacking cathepsin L are protected from cell puromycin aminonucleoside-induced cell detachment. The functional significance of cathepsin L expression was underscored by the observation that puromycin aminonucleoside-induced cell migration was slowed down in cathepsin L-deficient podocytes and by the preservation of cell-cell contacts and expression of vital slit diaphragm protein CD2AP. Cathepsin L expression and activity were induced in podocytes lacking alpha(3) integrin. Similarly, acute functional inhibition of alpha(3) integrin in wild type podocytes with a blocking antibody increased the expression of cathepsin L activity. Down-regulation of alpha(3) integrin protected against puromycin aminonucleoside-induced podocyte detachment. In summary, these data establish that podocyte foot process effacement is a migratory event involving a novel interplay between cathepsin L and alpha(3) integrin.",
keywords = "Adaptor Proteins, Signal Transducing, Animals, Blotting, Northern, Blotting, Western, Cathepsin L, Cathepsins/metabolism, Cell Differentiation, Cell Line, Cell Movement, Cells, Cultured, Cysteine Endopeptidases, Cytoskeletal Proteins, Down-Regulation, Extracellular Matrix/metabolism, Gene Expression Profiling, Humans, Immunoblotting, Integrin alpha3/metabolism, Integrins/metabolism, Kidney/metabolism, Male, Mice, Microscopy, Fluorescence, Models, Biological, Nephrotic Syndrome/metabolism, Polymerase Chain Reaction, Proteins/metabolism, Puromycin/pharmacology, Rats, Rats, Sprague-Dawley, Up-Regulation",
author = "Jochen Reiser and Jun Oh and Isao Shirato and Katsuhiko Asanuma and Andreas Hug and Mundel, {Thomas M} and Karen Honey and Kazumi Ishidoh and Eiki Kominami and Kreidberg, {Jordan A} and Yasuhiko Tomino and Peter Mundel",
year = "2004",
month = aug,
day = "13",
doi = "10.1074/jbc.M401973200",
language = "English",
volume = "279",
pages = "34827--32",
journal = "J BIOL CHEM",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology Inc.",
number = "33",

}

RIS

TY - JOUR

T1 - Podocyte migration during nephrotic syndrome requires a coordinated interplay between cathepsin L and alpha3 integrin

AU - Reiser, Jochen

AU - Oh, Jun

AU - Shirato, Isao

AU - Asanuma, Katsuhiko

AU - Hug, Andreas

AU - Mundel, Thomas M

AU - Honey, Karen

AU - Ishidoh, Kazumi

AU - Kominami, Eiki

AU - Kreidberg, Jordan A

AU - Tomino, Yasuhiko

AU - Mundel, Peter

PY - 2004/8/13

Y1 - 2004/8/13

N2 - Podocyte foot process effacement and disruption of the slit diaphragm are typically associated with glomerular proteinuria and can be induced in rats by the injection of puromycin aminonucleoside. Here, we show that the induction of puromycin aminonucleoside nephrosis involves podocyte migration conducted by a coordinated interplay between the cysteine protease cathepsin L and alpha(3) integrin. Puromycin aminonucleoside treatment up-regulates cathepsin L expression in podocytes in vivo as well as expression and enzymatic activity of cathepsin L in podocytes in vitro. Isolated podocytes from mice lacking cathepsin L are protected from cell puromycin aminonucleoside-induced cell detachment. The functional significance of cathepsin L expression was underscored by the observation that puromycin aminonucleoside-induced cell migration was slowed down in cathepsin L-deficient podocytes and by the preservation of cell-cell contacts and expression of vital slit diaphragm protein CD2AP. Cathepsin L expression and activity were induced in podocytes lacking alpha(3) integrin. Similarly, acute functional inhibition of alpha(3) integrin in wild type podocytes with a blocking antibody increased the expression of cathepsin L activity. Down-regulation of alpha(3) integrin protected against puromycin aminonucleoside-induced podocyte detachment. In summary, these data establish that podocyte foot process effacement is a migratory event involving a novel interplay between cathepsin L and alpha(3) integrin.

AB - Podocyte foot process effacement and disruption of the slit diaphragm are typically associated with glomerular proteinuria and can be induced in rats by the injection of puromycin aminonucleoside. Here, we show that the induction of puromycin aminonucleoside nephrosis involves podocyte migration conducted by a coordinated interplay between the cysteine protease cathepsin L and alpha(3) integrin. Puromycin aminonucleoside treatment up-regulates cathepsin L expression in podocytes in vivo as well as expression and enzymatic activity of cathepsin L in podocytes in vitro. Isolated podocytes from mice lacking cathepsin L are protected from cell puromycin aminonucleoside-induced cell detachment. The functional significance of cathepsin L expression was underscored by the observation that puromycin aminonucleoside-induced cell migration was slowed down in cathepsin L-deficient podocytes and by the preservation of cell-cell contacts and expression of vital slit diaphragm protein CD2AP. Cathepsin L expression and activity were induced in podocytes lacking alpha(3) integrin. Similarly, acute functional inhibition of alpha(3) integrin in wild type podocytes with a blocking antibody increased the expression of cathepsin L activity. Down-regulation of alpha(3) integrin protected against puromycin aminonucleoside-induced podocyte detachment. In summary, these data establish that podocyte foot process effacement is a migratory event involving a novel interplay between cathepsin L and alpha(3) integrin.

KW - Adaptor Proteins, Signal Transducing

KW - Animals

KW - Blotting, Northern

KW - Blotting, Western

KW - Cathepsin L

KW - Cathepsins/metabolism

KW - Cell Differentiation

KW - Cell Line

KW - Cell Movement

KW - Cells, Cultured

KW - Cysteine Endopeptidases

KW - Cytoskeletal Proteins

KW - Down-Regulation

KW - Extracellular Matrix/metabolism

KW - Gene Expression Profiling

KW - Humans

KW - Immunoblotting

KW - Integrin alpha3/metabolism

KW - Integrins/metabolism

KW - Kidney/metabolism

KW - Male

KW - Mice

KW - Microscopy, Fluorescence

KW - Models, Biological

KW - Nephrotic Syndrome/metabolism

KW - Polymerase Chain Reaction

KW - Proteins/metabolism

KW - Puromycin/pharmacology

KW - Rats

KW - Rats, Sprague-Dawley

KW - Up-Regulation

U2 - 10.1074/jbc.M401973200

DO - 10.1074/jbc.M401973200

M3 - SCORING: Journal article

C2 - 15197181

VL - 279

SP - 34827

EP - 34832

JO - J BIOL CHEM

JF - J BIOL CHEM

SN - 0021-9258

IS - 33

ER -