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Platelet derived bFGF mediates vascular integrative mechanisms of mesenchymal stem cells in vitro

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Platelet derived bFGF mediates vascular integrative mechanisms of mesenchymal stem cells in vitro. / Langer, Harald F; Stellos, Konstantinos; Steingen, Caroline; Froihofer, Amrei; Schönberger, Tanja; Krämer, Björn; Bigalke, Boris; May, Andreas E; Seizer, Peter; Müller, Iris; Gieseke, Friederike; Siegel-Axel, Dorothea; Meuth, Sven G; Schmidt, Annette; Wendel, Hans P; Müller, Ingo; Bloch, Wilhelm; Gawaz, Meinrad.

In: J MOL CELL CARDIOL, Vol. 47, No. 2, 08.2009, p. 315-25.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Langer, HF, Stellos, K, Steingen, C, Froihofer, A, Schönberger, T, Krämer, B, Bigalke, B, May, AE, Seizer, P, Müller, I, Gieseke, F, Siegel-Axel, D, Meuth, SG, Schmidt, A, Wendel, HP, Müller, I, Bloch, W & Gawaz, M 2009, 'Platelet derived bFGF mediates vascular integrative mechanisms of mesenchymal stem cells in vitro', J MOL CELL CARDIOL, vol. 47, no. 2, pp. 315-25. https://doi.org/10.1016/j.yjmcc.2009.03.011

APA

Langer, H. F., Stellos, K., Steingen, C., Froihofer, A., Schönberger, T., Krämer, B., Bigalke, B., May, A. E., Seizer, P., Müller, I., Gieseke, F., Siegel-Axel, D., Meuth, S. G., Schmidt, A., Wendel, H. P., Müller, I., Bloch, W., & Gawaz, M. (2009). Platelet derived bFGF mediates vascular integrative mechanisms of mesenchymal stem cells in vitro. J MOL CELL CARDIOL, 47(2), 315-25. https://doi.org/10.1016/j.yjmcc.2009.03.011

Vancouver

Langer HF, Stellos K, Steingen C, Froihofer A, Schönberger T, Krämer B et al. Platelet derived bFGF mediates vascular integrative mechanisms of mesenchymal stem cells in vitro. J MOL CELL CARDIOL. 2009 Aug;47(2):315-25. https://doi.org/10.1016/j.yjmcc.2009.03.011

Bibtex

@article{8459e577bb49408a96264335f1993154,
title = "Platelet derived bFGF mediates vascular integrative mechanisms of mesenchymal stem cells in vitro",
abstract = "Patients with myocardial infarction reveal an altered number of circulating mesenchymal stem cells (MSCs). Recently, it was shown that MSCs are able to regenerate myocardial tissue and to differentiate into endothelial cells. The homing mechanisms of MSCs from the circulation into the target tissue, however, are not understood so far. In this study, we evaluated the impact of platelets on MSC recruitment, proliferation, migration and integration into the endothelium. MSCs expressing alpha(v)beta(3) integrin were recruited to human arterial endothelial cells exposed to isolated platelets or IL-1 beta under high shear conditions. Furthermore, induction of vascular injury in vivo resulted in increased recruitment of injected MSCs as assessed by intravital microscopy and depletion of platelets significantly reduced this adhesion. The interaction of platelets and MSCs was inhibited by pre-incubation with the mAb 7E3 or an RGD protein both blocking beta(3) integrin mediated adhesion. Platelets had a chemotactic effect on MSCs, promoted a migratory MSC phenotype and dose- and activation-dependently enhanced migration of MSCs, a process, which was mediated by basic fibroblast growth factor (bFGF). Similarly, platelet derived bFGF increased proliferation of MSCs. Coincubation of MSCs with platelets facilitated integration into an endothelial monolayer, which was significantly reduced by pre-incubation with a blocking mAb to bFGF. We conclude that platelets may play a critical part in the recruitment of MSCs to the endothelium, influence MSC function and promote integration of MSCs into the endothelium.",
keywords = "Animals, Arteries, Blood Platelets, Blood Vessels, Cell Adhesion, Cell Movement, Cell Proliferation, Endothelial Cells, Fibroblast Growth Factor 2, Humans, Integrin alphaVbeta3, Mesenchymal Stromal Cells, Mice, Shear Strength",
author = "Langer, {Harald F} and Konstantinos Stellos and Caroline Steingen and Amrei Froihofer and Tanja Sch{\"o}nberger and Bj{\"o}rn Kr{\"a}mer and Boris Bigalke and May, {Andreas E} and Peter Seizer and Iris M{\"u}ller and Friederike Gieseke and Dorothea Siegel-Axel and Meuth, {Sven G} and Annette Schmidt and Wendel, {Hans P} and Ingo M{\"u}ller and Wilhelm Bloch and Meinrad Gawaz",
year = "2009",
month = aug,
doi = "10.1016/j.yjmcc.2009.03.011",
language = "English",
volume = "47",
pages = "315--25",
journal = "J MOL CELL CARDIOL",
issn = "0022-2828",
publisher = "Academic Press Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Platelet derived bFGF mediates vascular integrative mechanisms of mesenchymal stem cells in vitro

AU - Langer, Harald F

AU - Stellos, Konstantinos

AU - Steingen, Caroline

AU - Froihofer, Amrei

AU - Schönberger, Tanja

AU - Krämer, Björn

AU - Bigalke, Boris

AU - May, Andreas E

AU - Seizer, Peter

AU - Müller, Iris

AU - Gieseke, Friederike

AU - Siegel-Axel, Dorothea

AU - Meuth, Sven G

AU - Schmidt, Annette

AU - Wendel, Hans P

AU - Müller, Ingo

AU - Bloch, Wilhelm

AU - Gawaz, Meinrad

PY - 2009/8

Y1 - 2009/8

N2 - Patients with myocardial infarction reveal an altered number of circulating mesenchymal stem cells (MSCs). Recently, it was shown that MSCs are able to regenerate myocardial tissue and to differentiate into endothelial cells. The homing mechanisms of MSCs from the circulation into the target tissue, however, are not understood so far. In this study, we evaluated the impact of platelets on MSC recruitment, proliferation, migration and integration into the endothelium. MSCs expressing alpha(v)beta(3) integrin were recruited to human arterial endothelial cells exposed to isolated platelets or IL-1 beta under high shear conditions. Furthermore, induction of vascular injury in vivo resulted in increased recruitment of injected MSCs as assessed by intravital microscopy and depletion of platelets significantly reduced this adhesion. The interaction of platelets and MSCs was inhibited by pre-incubation with the mAb 7E3 or an RGD protein both blocking beta(3) integrin mediated adhesion. Platelets had a chemotactic effect on MSCs, promoted a migratory MSC phenotype and dose- and activation-dependently enhanced migration of MSCs, a process, which was mediated by basic fibroblast growth factor (bFGF). Similarly, platelet derived bFGF increased proliferation of MSCs. Coincubation of MSCs with platelets facilitated integration into an endothelial monolayer, which was significantly reduced by pre-incubation with a blocking mAb to bFGF. We conclude that platelets may play a critical part in the recruitment of MSCs to the endothelium, influence MSC function and promote integration of MSCs into the endothelium.

AB - Patients with myocardial infarction reveal an altered number of circulating mesenchymal stem cells (MSCs). Recently, it was shown that MSCs are able to regenerate myocardial tissue and to differentiate into endothelial cells. The homing mechanisms of MSCs from the circulation into the target tissue, however, are not understood so far. In this study, we evaluated the impact of platelets on MSC recruitment, proliferation, migration and integration into the endothelium. MSCs expressing alpha(v)beta(3) integrin were recruited to human arterial endothelial cells exposed to isolated platelets or IL-1 beta under high shear conditions. Furthermore, induction of vascular injury in vivo resulted in increased recruitment of injected MSCs as assessed by intravital microscopy and depletion of platelets significantly reduced this adhesion. The interaction of platelets and MSCs was inhibited by pre-incubation with the mAb 7E3 or an RGD protein both blocking beta(3) integrin mediated adhesion. Platelets had a chemotactic effect on MSCs, promoted a migratory MSC phenotype and dose- and activation-dependently enhanced migration of MSCs, a process, which was mediated by basic fibroblast growth factor (bFGF). Similarly, platelet derived bFGF increased proliferation of MSCs. Coincubation of MSCs with platelets facilitated integration into an endothelial monolayer, which was significantly reduced by pre-incubation with a blocking mAb to bFGF. We conclude that platelets may play a critical part in the recruitment of MSCs to the endothelium, influence MSC function and promote integration of MSCs into the endothelium.

KW - Animals

KW - Arteries

KW - Blood Platelets

KW - Blood Vessels

KW - Cell Adhesion

KW - Cell Movement

KW - Cell Proliferation

KW - Endothelial Cells

KW - Fibroblast Growth Factor 2

KW - Humans

KW - Integrin alphaVbeta3

KW - Mesenchymal Stromal Cells

KW - Mice

KW - Shear Strength

U2 - 10.1016/j.yjmcc.2009.03.011

DO - 10.1016/j.yjmcc.2009.03.011

M3 - SCORING: Journal article

C2 - 19328809

VL - 47

SP - 315

EP - 325

JO - J MOL CELL CARDIOL

JF - J MOL CELL CARDIOL

SN - 0022-2828

IS - 2

ER -