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Plasmacytoid dendritic cells accumulate and secrete interferon alpha in lymph nodes of HIV-1 patients.

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Plasmacytoid dendritic cells accumulate and secrete interferon alpha in lymph nodes of HIV-1 patients. / Lehmann, Clara; Lafferty, Mark; Garzino-Demo, Alfredo; Jung, Norma; Hartmann, Pia; Fätkenheuer, Gerd; Wolf, Jeffrey S; van Lunzen, Jan; Romerio, Fabio.

In: PLOS ONE, Vol. 5, No. 6, 6, 2010, p. 11110.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Lehmann, C, Lafferty, M, Garzino-Demo, A, Jung, N, Hartmann, P, Fätkenheuer, G, Wolf, JS, van Lunzen, J & Romerio, F 2010, 'Plasmacytoid dendritic cells accumulate and secrete interferon alpha in lymph nodes of HIV-1 patients.', PLOS ONE, vol. 5, no. 6, 6, pp. 11110. https://doi.org/10.1371/journal.pone.0011110

APA

Lehmann, C., Lafferty, M., Garzino-Demo, A., Jung, N., Hartmann, P., Fätkenheuer, G., Wolf, J. S., van Lunzen, J., & Romerio, F. (2010). Plasmacytoid dendritic cells accumulate and secrete interferon alpha in lymph nodes of HIV-1 patients. PLOS ONE, 5(6), 11110. [6]. https://doi.org/10.1371/journal.pone.0011110

Vancouver

Lehmann C, Lafferty M, Garzino-Demo A, Jung N, Hartmann P, Fätkenheuer G et al. Plasmacytoid dendritic cells accumulate and secrete interferon alpha in lymph nodes of HIV-1 patients. PLOS ONE. 2010;5(6):11110. 6. https://doi.org/10.1371/journal.pone.0011110

Bibtex

@article{4deb4ede2d554de89e765ff0fd38cdaa,
title = "Plasmacytoid dendritic cells accumulate and secrete interferon alpha in lymph nodes of HIV-1 patients.",
abstract = "Circulating plasmacytoid dendritic cells (pDC) decline during HIV-1 infection, but at the same time they express markedly higher levels of interferon alpha (IFNalpha), which is associated with HIV-1 disease progression. Here we show an accumulation of pDC in lymph nodes (LN) of treatment-na{\"i}ve HIV-1 patients. This phenomenon was associated with elevated expression of the LN homing marker, CCR7, on pDC in peripheral blood of HIV-1 patients, which conferred increased migratory capacity in response to CCR7 ligands in ex vivo functional assays. LN-homed pDC of HIV-1 patients presented higher CD40 and lower BDCA2 levels, but unchanged CD83 and CD86 expression. In addition, these cells expressed markedly higher amounts of IFNalpha compared to uninfected individuals, and were undergoing faster rates of cell death. These results demonstrate for the first time that in asymptomatic, untreated HIV-1 patients circulating pDC up-regulate CCR7 expression, accumulate in lymph nodes, and express high amounts of IFNalpha before undergoing cell death. Since IFNalpha inhibits cell proliferation and modulates immune responses, chronically high levels of this cytokine in LN of HIV-1 patients may impair differentiation and immune function of bystander CD4(+) T cells, thus playing into the mechanisms of AIDS immunopathogenesis.",
keywords = "Adult, Humans, Male, Female, HIV-1 isolation, purification, Base Sequence, Flow Cytometry, DNA Primers, Dendritic Cells secretion, HIV Infections metabolism, Interferon-alpha secretion, Lymph Nodes secretion, Receptors, CCR7 blood, Adult, Humans, Male, Female, HIV-1 isolation, purification, Base Sequence, Flow Cytometry, DNA Primers, Dendritic Cells secretion, HIV Infections metabolism, Interferon-alpha secretion, Lymph Nodes secretion, Receptors, CCR7 blood",
author = "Clara Lehmann and Mark Lafferty and Alfredo Garzino-Demo and Norma Jung and Pia Hartmann and Gerd F{\"a}tkenheuer and Wolf, {Jeffrey S} and {van Lunzen}, Jan and Fabio Romerio",
year = "2010",
doi = "10.1371/journal.pone.0011110",
language = "Deutsch",
volume = "5",
pages = "11110",
journal = "PLOS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "6",

}

RIS

TY - JOUR

T1 - Plasmacytoid dendritic cells accumulate and secrete interferon alpha in lymph nodes of HIV-1 patients.

AU - Lehmann, Clara

AU - Lafferty, Mark

AU - Garzino-Demo, Alfredo

AU - Jung, Norma

AU - Hartmann, Pia

AU - Fätkenheuer, Gerd

AU - Wolf, Jeffrey S

AU - van Lunzen, Jan

AU - Romerio, Fabio

PY - 2010

Y1 - 2010

N2 - Circulating plasmacytoid dendritic cells (pDC) decline during HIV-1 infection, but at the same time they express markedly higher levels of interferon alpha (IFNalpha), which is associated with HIV-1 disease progression. Here we show an accumulation of pDC in lymph nodes (LN) of treatment-naïve HIV-1 patients. This phenomenon was associated with elevated expression of the LN homing marker, CCR7, on pDC in peripheral blood of HIV-1 patients, which conferred increased migratory capacity in response to CCR7 ligands in ex vivo functional assays. LN-homed pDC of HIV-1 patients presented higher CD40 and lower BDCA2 levels, but unchanged CD83 and CD86 expression. In addition, these cells expressed markedly higher amounts of IFNalpha compared to uninfected individuals, and were undergoing faster rates of cell death. These results demonstrate for the first time that in asymptomatic, untreated HIV-1 patients circulating pDC up-regulate CCR7 expression, accumulate in lymph nodes, and express high amounts of IFNalpha before undergoing cell death. Since IFNalpha inhibits cell proliferation and modulates immune responses, chronically high levels of this cytokine in LN of HIV-1 patients may impair differentiation and immune function of bystander CD4(+) T cells, thus playing into the mechanisms of AIDS immunopathogenesis.

AB - Circulating plasmacytoid dendritic cells (pDC) decline during HIV-1 infection, but at the same time they express markedly higher levels of interferon alpha (IFNalpha), which is associated with HIV-1 disease progression. Here we show an accumulation of pDC in lymph nodes (LN) of treatment-naïve HIV-1 patients. This phenomenon was associated with elevated expression of the LN homing marker, CCR7, on pDC in peripheral blood of HIV-1 patients, which conferred increased migratory capacity in response to CCR7 ligands in ex vivo functional assays. LN-homed pDC of HIV-1 patients presented higher CD40 and lower BDCA2 levels, but unchanged CD83 and CD86 expression. In addition, these cells expressed markedly higher amounts of IFNalpha compared to uninfected individuals, and were undergoing faster rates of cell death. These results demonstrate for the first time that in asymptomatic, untreated HIV-1 patients circulating pDC up-regulate CCR7 expression, accumulate in lymph nodes, and express high amounts of IFNalpha before undergoing cell death. Since IFNalpha inhibits cell proliferation and modulates immune responses, chronically high levels of this cytokine in LN of HIV-1 patients may impair differentiation and immune function of bystander CD4(+) T cells, thus playing into the mechanisms of AIDS immunopathogenesis.

KW - Adult

KW - Humans

KW - Male

KW - Female

KW - HIV-1 isolation

KW - purification

KW - Base Sequence

KW - Flow Cytometry

KW - DNA Primers

KW - Dendritic Cells secretion

KW - HIV Infections metabolism

KW - Interferon-alpha secretion

KW - Lymph Nodes secretion

KW - Receptors, CCR7 blood

KW - Adult

KW - Humans

KW - Male

KW - Female

KW - HIV-1 isolation

KW - purification

KW - Base Sequence

KW - Flow Cytometry

KW - DNA Primers

KW - Dendritic Cells secretion

KW - HIV Infections metabolism

KW - Interferon-alpha secretion

KW - Lymph Nodes secretion

KW - Receptors, CCR7 blood

U2 - 10.1371/journal.pone.0011110

DO - 10.1371/journal.pone.0011110

M3 - SCORING: Zeitschriftenaufsatz

VL - 5

SP - 11110

JO - PLOS ONE

JF - PLOS ONE

SN - 1932-6203

IS - 6

M1 - 6

ER -