Plasma medicine and wound management
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Plasma medicine and wound management : Evaluation of the antibacterial efficacy of a medically certified cold atmospheric argon plasma jet. / Plattfaut, Isabell; Besser, Manuela; Severing, Anna-Lena; Stürmer, Ewa K; Opländer, Christian.
In: INT J ANTIMICROB AG, Vol. 57, No. 5, 106319, 05.05.2021.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Plasma medicine and wound management
T2 - Evaluation of the antibacterial efficacy of a medically certified cold atmospheric argon plasma jet
AU - Plattfaut, Isabell
AU - Besser, Manuela
AU - Severing, Anna-Lena
AU - Stürmer, Ewa K
AU - Opländer, Christian
N1 - Copyright © 2021 Elsevier Ltd. All rights reserved.
PY - 2021/5/5
Y1 - 2021/5/5
N2 - OBJECTIVES: A major problem for wound healing is contamination with bacteria, often resulting in biofilm formation and wound infection, which, in turn, needs immediate intervention such as surgical debridement and through irrigation. A topical treatment with cold atmospheric pressure plasma (CAP) for wound disinfection may present an alternative and less painful approach.METHODS: This study investigated the antibacterial effects of a cold atmospheric pressure argon plasma jet (kINPen® MED) as a CAP source, using the three-dimensional Staphylococcus aureus immunocompetent biofilm system hpBIOM in addition to a standard planktonic test. Furthermore, skin cell compatibility was evaluated using a keratinocyte (HaCat) model.RESULTS: CAP treatment (0-240 s) followed by incubation (15, 120 min) within the CAP-treated media showed slight bactericidal efficacy under planktonic conditions but no effect on biofilms. However, indirect CAP treatment of keratinocytes performed under the same conditions resulted in a significant decrease in metabolic activity. Short CAP treatment and exposure time (30 s; 15 min) induced a slight increase in the metabolic activity; however, longer treatments and/or exposure times led to pronounced reductions up to 100%. These effects could partially be reversed by addition of catalase, indicating a dominant role of CAP-generated hydrogen peroxide.CONCLUSIONS: These results indicate that plasma treatment does not lead to the desired disinfection or significant reduction in the bacterial burden of Staphylococcus aureus in a wet milieu or in biofilms. Thus, treatment with CAP could not be recommended as a single anti-bacterial therapy for wounds but could be used to support standard treatments.
AB - OBJECTIVES: A major problem for wound healing is contamination with bacteria, often resulting in biofilm formation and wound infection, which, in turn, needs immediate intervention such as surgical debridement and through irrigation. A topical treatment with cold atmospheric pressure plasma (CAP) for wound disinfection may present an alternative and less painful approach.METHODS: This study investigated the antibacterial effects of a cold atmospheric pressure argon plasma jet (kINPen® MED) as a CAP source, using the three-dimensional Staphylococcus aureus immunocompetent biofilm system hpBIOM in addition to a standard planktonic test. Furthermore, skin cell compatibility was evaluated using a keratinocyte (HaCat) model.RESULTS: CAP treatment (0-240 s) followed by incubation (15, 120 min) within the CAP-treated media showed slight bactericidal efficacy under planktonic conditions but no effect on biofilms. However, indirect CAP treatment of keratinocytes performed under the same conditions resulted in a significant decrease in metabolic activity. Short CAP treatment and exposure time (30 s; 15 min) induced a slight increase in the metabolic activity; however, longer treatments and/or exposure times led to pronounced reductions up to 100%. These effects could partially be reversed by addition of catalase, indicating a dominant role of CAP-generated hydrogen peroxide.CONCLUSIONS: These results indicate that plasma treatment does not lead to the desired disinfection or significant reduction in the bacterial burden of Staphylococcus aureus in a wet milieu or in biofilms. Thus, treatment with CAP could not be recommended as a single anti-bacterial therapy for wounds but could be used to support standard treatments.
KW - Anti-Bacterial Agents/pharmacology
KW - Argon/pharmacology
KW - Atmospheric Pressure
KW - Biofilms/drug effects
KW - HaCaT Cells
KW - Humans
KW - Keratinocytes/drug effects
KW - Microbial Viability
KW - Plasma Gases/pharmacology
KW - Skin/drug effects
KW - Staphylococcus aureus/cytology
KW - Wound Healing/drug effects
KW - Wound Infection/microbiology
U2 - 10.1016/j.ijantimicag.2021.106319
DO - 10.1016/j.ijantimicag.2021.106319
M3 - SCORING: Journal article
C2 - 33716180
VL - 57
JO - INT J ANTIMICROB AG
JF - INT J ANTIMICROB AG
SN - 0924-8579
IS - 5
M1 - 106319
ER -