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Pinching the cortex of live cells reveals thickness instabilities caused by myosin II motors

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Pinching the cortex of live cells reveals thickness instabilities caused by myosin II motors. / Laplaud, Valentin; Levernier, Nicolas; Pineau, Judith; Roman, Mabel San; Barbier, Lucie; Sáez, Pablo J; Lennon-Duménil, Ana-Maria; Vargas, Pablo; Kruse, Karsten; du Roure, Olivia; Piel, Matthieu; Heuvingh, Julien.

In: SCI ADV, Vol. 7, No. 27, eabe3640, 07.2021.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Laplaud, V, Levernier, N, Pineau, J, Roman, MS, Barbier, L, Sáez, PJ, Lennon-Duménil, A-M, Vargas, P, Kruse, K, du Roure, O, Piel, M & Heuvingh, J 2021, 'Pinching the cortex of live cells reveals thickness instabilities caused by myosin II motors', SCI ADV, vol. 7, no. 27, eabe3640. https://doi.org/10.1126/sciadv.abe3640

APA

Laplaud, V., Levernier, N., Pineau, J., Roman, M. S., Barbier, L., Sáez, P. J., Lennon-Duménil, A-M., Vargas, P., Kruse, K., du Roure, O., Piel, M., & Heuvingh, J. (2021). Pinching the cortex of live cells reveals thickness instabilities caused by myosin II motors. SCI ADV, 7(27), [eabe3640]. https://doi.org/10.1126/sciadv.abe3640

Vancouver

Laplaud V, Levernier N, Pineau J, Roman MS, Barbier L, Sáez PJ et al. Pinching the cortex of live cells reveals thickness instabilities caused by myosin II motors. SCI ADV. 2021 Jul;7(27). eabe3640. https://doi.org/10.1126/sciadv.abe3640

Bibtex

@article{9e072f9513544e9fbbc2baf863d17fd2,
title = "Pinching the cortex of live cells reveals thickness instabilities caused by myosin II motors",
abstract = "The cell cortex is a contractile actin meshwork, which determines cell shape and is essential for cell mechanics, migration, and division. Because its thickness is below optical resolution, there is a tendency to consider the cortex as a thin uniform two-dimensional layer. Using two mutually attracted magnetic beads, one inside the cell and the other in the extracellular medium, we pinch the cortex of dendritic cells and provide an accurate and time-resolved measure of its thickness. Our observations draw a new picture of the cell cortex as a highly dynamic layer, harboring large fluctuations in its third dimension because of actomyosin contractility. We propose that the cortex dynamics might be responsible for the fast shape-changing capacity of highly contractile cells that use amoeboid-like migration.",
author = "Valentin Laplaud and Nicolas Levernier and Judith Pineau and Roman, {Mabel San} and Lucie Barbier and S{\'a}ez, {Pablo J} and Ana-Maria Lennon-Dum{\'e}nil and Pablo Vargas and Karsten Kruse and {du Roure}, Olivia and Matthieu Piel and Julien Heuvingh",
note = "Copyright {\textcopyright} 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).",
year = "2021",
month = jul,
doi = "10.1126/sciadv.abe3640",
language = "English",
volume = "7",
journal = "SCI ADV",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "27",

}

RIS

TY - JOUR

T1 - Pinching the cortex of live cells reveals thickness instabilities caused by myosin II motors

AU - Laplaud, Valentin

AU - Levernier, Nicolas

AU - Pineau, Judith

AU - Roman, Mabel San

AU - Barbier, Lucie

AU - Sáez, Pablo J

AU - Lennon-Duménil, Ana-Maria

AU - Vargas, Pablo

AU - Kruse, Karsten

AU - du Roure, Olivia

AU - Piel, Matthieu

AU - Heuvingh, Julien

N1 - Copyright © 2021 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC).

PY - 2021/7

Y1 - 2021/7

N2 - The cell cortex is a contractile actin meshwork, which determines cell shape and is essential for cell mechanics, migration, and division. Because its thickness is below optical resolution, there is a tendency to consider the cortex as a thin uniform two-dimensional layer. Using two mutually attracted magnetic beads, one inside the cell and the other in the extracellular medium, we pinch the cortex of dendritic cells and provide an accurate and time-resolved measure of its thickness. Our observations draw a new picture of the cell cortex as a highly dynamic layer, harboring large fluctuations in its third dimension because of actomyosin contractility. We propose that the cortex dynamics might be responsible for the fast shape-changing capacity of highly contractile cells that use amoeboid-like migration.

AB - The cell cortex is a contractile actin meshwork, which determines cell shape and is essential for cell mechanics, migration, and division. Because its thickness is below optical resolution, there is a tendency to consider the cortex as a thin uniform two-dimensional layer. Using two mutually attracted magnetic beads, one inside the cell and the other in the extracellular medium, we pinch the cortex of dendritic cells and provide an accurate and time-resolved measure of its thickness. Our observations draw a new picture of the cell cortex as a highly dynamic layer, harboring large fluctuations in its third dimension because of actomyosin contractility. We propose that the cortex dynamics might be responsible for the fast shape-changing capacity of highly contractile cells that use amoeboid-like migration.

U2 - 10.1126/sciadv.abe3640

DO - 10.1126/sciadv.abe3640

M3 - SCORING: Journal article

C2 - 34215576

VL - 7

JO - SCI ADV

JF - SCI ADV

SN - 2375-2548

IS - 27

M1 - eabe3640

ER -