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Partial hepatectomy induces mobilization of a unique population of haematopoietic progenitor cells in human healthy liver donors.

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Partial hepatectomy induces mobilization of a unique population of haematopoietic progenitor cells in human healthy liver donors. / Gehling, Ursula; Willems, Marc; Dandri-Petersen, Maura; Petersen, Jörg; Berna, Marc; Thill, Michelle; Wulf, Tina; Müller, Lars; Pollok, Jörg-Matthias; Schlagner, Kathleen; Faltz, Christiane; Hossfeld, Dieter K; Rogiers, Xavier.

In: J HEPATOL, Vol. 43, No. 5, 5, 2005, p. 845-853.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Gehling, U, Willems, M, Dandri-Petersen, M, Petersen, J, Berna, M, Thill, M, Wulf, T, Müller, L, Pollok, J-M, Schlagner, K, Faltz, C, Hossfeld, DK & Rogiers, X 2005, 'Partial hepatectomy induces mobilization of a unique population of haematopoietic progenitor cells in human healthy liver donors.', J HEPATOL, vol. 43, no. 5, 5, pp. 845-853. <http://www.ncbi.nlm.nih.gov/pubmed/16139387?dopt=Citation>

APA

Gehling, U., Willems, M., Dandri-Petersen, M., Petersen, J., Berna, M., Thill, M., Wulf, T., Müller, L., Pollok, J-M., Schlagner, K., Faltz, C., Hossfeld, D. K., & Rogiers, X. (2005). Partial hepatectomy induces mobilization of a unique population of haematopoietic progenitor cells in human healthy liver donors. J HEPATOL, 43(5), 845-853. [5]. http://www.ncbi.nlm.nih.gov/pubmed/16139387?dopt=Citation

Vancouver

Gehling U, Willems M, Dandri-Petersen M, Petersen J, Berna M, Thill M et al. Partial hepatectomy induces mobilization of a unique population of haematopoietic progenitor cells in human healthy liver donors. J HEPATOL. 2005;43(5):845-853. 5.

Bibtex

@article{f2360d93f38049c8b1686b32c3068da7,
title = "Partial hepatectomy induces mobilization of a unique population of haematopoietic progenitor cells in human healthy liver donors.",
abstract = "BACKGROUND/AIMS: Recent studies indicate that after transplantation, circulating bone marrow-derived stem cells migrate into the liver and contribute to liver regeneration. Whether such cells are actively recruited from the bone marrow for liver repair remains to be determined. In this regard, we investigated whether liver resection leads to a release of stem cell marker-positive (+) cells into the peripheral circulation. METHODS: Peripheral blood samples from 11 living liver donors were analyzed by flow cytometry one day before and 12h after partial hepatectomy (PH) using antibodies against CD133, CD34, CD45, CD14, c-kit, bcrp-1. Immunomagnetic separation was performed to select CD133+ cells for functional assays in vitro. RESULTS: A significant increase in the percentage of CD133+ cells could be demonstrated in all donors studied. Unexpectedly, virtually all CD133+ cells coexpressed CD45 and CD14, whereas only a small subset expressed CD34. No significant staining was observed for c-kit and bcrp-1. In culture, immunoselected CD133+ cells displayed characteristics of myelomonocytic precursors. In addition, enriched CD133+ generated an adherent cell population that expressed CK8, alpha-fetoprotein, and human albumin. CONCLUSIONS: PH induces mobilization of a distinct population of myelomonocytic progenitor cells, which have hepatic differentiation potential in vitro, and might play a role in liver regeneration after PH in humans.",
author = "Ursula Gehling and Marc Willems and Maura Dandri-Petersen and J{\"o}rg Petersen and Marc Berna and Michelle Thill and Tina Wulf and Lars M{\"u}ller and J{\"o}rg-Matthias Pollok and Kathleen Schlagner and Christiane Faltz and Hossfeld, {Dieter K} and Xavier Rogiers",
year = "2005",
language = "Deutsch",
volume = "43",
pages = "845--853",
journal = "J HEPATOL",
issn = "0168-8278",
publisher = "Elsevier",
number = "5",

}

RIS

TY - JOUR

T1 - Partial hepatectomy induces mobilization of a unique population of haematopoietic progenitor cells in human healthy liver donors.

AU - Gehling, Ursula

AU - Willems, Marc

AU - Dandri-Petersen, Maura

AU - Petersen, Jörg

AU - Berna, Marc

AU - Thill, Michelle

AU - Wulf, Tina

AU - Müller, Lars

AU - Pollok, Jörg-Matthias

AU - Schlagner, Kathleen

AU - Faltz, Christiane

AU - Hossfeld, Dieter K

AU - Rogiers, Xavier

PY - 2005

Y1 - 2005

N2 - BACKGROUND/AIMS: Recent studies indicate that after transplantation, circulating bone marrow-derived stem cells migrate into the liver and contribute to liver regeneration. Whether such cells are actively recruited from the bone marrow for liver repair remains to be determined. In this regard, we investigated whether liver resection leads to a release of stem cell marker-positive (+) cells into the peripheral circulation. METHODS: Peripheral blood samples from 11 living liver donors were analyzed by flow cytometry one day before and 12h after partial hepatectomy (PH) using antibodies against CD133, CD34, CD45, CD14, c-kit, bcrp-1. Immunomagnetic separation was performed to select CD133+ cells for functional assays in vitro. RESULTS: A significant increase in the percentage of CD133+ cells could be demonstrated in all donors studied. Unexpectedly, virtually all CD133+ cells coexpressed CD45 and CD14, whereas only a small subset expressed CD34. No significant staining was observed for c-kit and bcrp-1. In culture, immunoselected CD133+ cells displayed characteristics of myelomonocytic precursors. In addition, enriched CD133+ generated an adherent cell population that expressed CK8, alpha-fetoprotein, and human albumin. CONCLUSIONS: PH induces mobilization of a distinct population of myelomonocytic progenitor cells, which have hepatic differentiation potential in vitro, and might play a role in liver regeneration after PH in humans.

AB - BACKGROUND/AIMS: Recent studies indicate that after transplantation, circulating bone marrow-derived stem cells migrate into the liver and contribute to liver regeneration. Whether such cells are actively recruited from the bone marrow for liver repair remains to be determined. In this regard, we investigated whether liver resection leads to a release of stem cell marker-positive (+) cells into the peripheral circulation. METHODS: Peripheral blood samples from 11 living liver donors were analyzed by flow cytometry one day before and 12h after partial hepatectomy (PH) using antibodies against CD133, CD34, CD45, CD14, c-kit, bcrp-1. Immunomagnetic separation was performed to select CD133+ cells for functional assays in vitro. RESULTS: A significant increase in the percentage of CD133+ cells could be demonstrated in all donors studied. Unexpectedly, virtually all CD133+ cells coexpressed CD45 and CD14, whereas only a small subset expressed CD34. No significant staining was observed for c-kit and bcrp-1. In culture, immunoselected CD133+ cells displayed characteristics of myelomonocytic precursors. In addition, enriched CD133+ generated an adherent cell population that expressed CK8, alpha-fetoprotein, and human albumin. CONCLUSIONS: PH induces mobilization of a distinct population of myelomonocytic progenitor cells, which have hepatic differentiation potential in vitro, and might play a role in liver regeneration after PH in humans.

M3 - SCORING: Zeitschriftenaufsatz

VL - 43

SP - 845

EP - 853

JO - J HEPATOL

JF - J HEPATOL

SN - 0168-8278

IS - 5

M1 - 5

ER -