Parameter study of shipping conditions for the ready-to-use application of a 3D human hemicornea construct in drug absorption studies
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Parameter study of shipping conditions for the ready-to-use application of a 3D human hemicornea construct in drug absorption studies. / Beißner, Nicole; Zorn-Kruppa, Michaela; Reichl, Stephan.
In: INT J PHARMACEUT, Vol. 536, No. 1, 30.01.2018, p. 377-387.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
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TY - JOUR
T1 - Parameter study of shipping conditions for the ready-to-use application of a 3D human hemicornea construct in drug absorption studies
AU - Beißner, Nicole
AU - Zorn-Kruppa, Michaela
AU - Reichl, Stephan
N1 - Copyright © 2017 Elsevier B.V. All rights reserved.
PY - 2018/1/30
Y1 - 2018/1/30
N2 - In this study, a shipping protocol for our 3D human hemicornea (HC) construct should be developed to provide quality-maintaining shipping conditions and to allow its ready-to-use application in drug absorption studies. First, the effects of single and multiple parameters, such as the type of shipping container, storage temperature and CO2 supply, were investigated under controlled laboratory conditions by assessing cell viability via MTT dye reaction and epithelial barrier properties via transepithelial electrical resistance (TEER) measurements. These investigations showed that TEER is more susceptible to shipping parameters than cell viability. Furthermore, the results were used to determine the optimal shipping conditions and critical values for subsequent overnight, real-time shipping experiments. Epithelial barrier properties were then investigated via TEER and the permeation of sodium fluorescein for shipped and not shipped HC. The results underscore that acceleration forces and changes in position may have a great impact on the epithelial barrier of 3D models. Low acceleration values and short changes in position caused only minor impairments. However, combined or intensive separate effects resulted in considerably low yields after shipping. Consequently, barrier-maintaining shipping of 3D in vitro models seems to be challenging, as mechanical forces have to be reduced to a minimum.
AB - In this study, a shipping protocol for our 3D human hemicornea (HC) construct should be developed to provide quality-maintaining shipping conditions and to allow its ready-to-use application in drug absorption studies. First, the effects of single and multiple parameters, such as the type of shipping container, storage temperature and CO2 supply, were investigated under controlled laboratory conditions by assessing cell viability via MTT dye reaction and epithelial barrier properties via transepithelial electrical resistance (TEER) measurements. These investigations showed that TEER is more susceptible to shipping parameters than cell viability. Furthermore, the results were used to determine the optimal shipping conditions and critical values for subsequent overnight, real-time shipping experiments. Epithelial barrier properties were then investigated via TEER and the permeation of sodium fluorescein for shipped and not shipped HC. The results underscore that acceleration forces and changes in position may have a great impact on the epithelial barrier of 3D models. Low acceleration values and short changes in position caused only minor impairments. However, combined or intensive separate effects resulted in considerably low yields after shipping. Consequently, barrier-maintaining shipping of 3D in vitro models seems to be challenging, as mechanical forces have to be reduced to a minimum.
KW - Journal Article
U2 - 10.1016/j.ijpharm.2017.11.057
DO - 10.1016/j.ijpharm.2017.11.057
M3 - SCORING: Journal article
C2 - 29191484
VL - 536
SP - 377
EP - 387
JO - INT J PHARMACEUT
JF - INT J PHARMACEUT
SN - 0378-5173
IS - 1
ER -
