Parallel assessment of Th17 cell frequencies by surface marker co-expression versus ex vivo IL-17 production in HIV-1 infection
Standard
Parallel assessment of Th17 cell frequencies by surface marker co-expression versus ex vivo IL-17 production in HIV-1 infection. / Dunay, Gabor Artur; Tóth, Ilona; Eberhard, Johanna M; Degen, Olaf; Tolosa, Eva; Lunzen, Jan; Hauber, Joachim; Zur Wiesch, Julian Schulze.
In: CYTOM PART B-CLIN CY, Vol. 90, No. 6, 11.2016, p. 486-492.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - Parallel assessment of Th17 cell frequencies by surface marker co-expression versus ex vivo IL-17 production in HIV-1 infection
AU - Dunay, Gabor Artur
AU - Tóth, Ilona
AU - Eberhard, Johanna M
AU - Degen, Olaf
AU - Tolosa, Eva
AU - Lunzen, Jan
AU - Hauber, Joachim
AU - Zur Wiesch, Julian Schulze
N1 - © 2015 International Clinical Cytometry Society.
PY - 2016/11
Y1 - 2016/11
N2 - INTRODUCTION: Th17 cells can either be identified by co-staining of surface markers or by intracellular cytokine staining (ICS) for IL-17 production. Discrepancies regarding the published frequencies of Th17 cells in peripheral blood mononuclear cells (PBMC) of HIV patients may partly be due to the different methodologies used.METHODS: Cryopreserved PBMCs from healthy controls and HIV infected subjects, including treated (cART) and viremic patients, were split and analyzed side by side by flow cytometry for expression of surface markers CCR6, CXCR3, CCR4 and CD161, or for intracellular expression of IL-17A and IFNγ after stimulation.RESULTS: The characterization of Th17 cells as CCR6+CXCR3-CCR4+CD161+ yielded considerably higher frequencies than the corresponding frequencies obtained by characterization via cytokines (IL-17+IFNγ-), regardless of the HIV status. However, the overall frequencies delivered by the two methods significantly correlated. The relative frequency of Th17 cells within the CD4+ T cell compartment was preserved in HIV infection, but there was a significant decrease in the absolute Th17 number, which was restored after initiation of cART, paralleling CD4+ T cell recovery. Absolute Th17 numbers inversely correlated with HIV viral load.CONCLUSION: The definition of Th17 cells by surface markers might overestimate their frequency in comparison to functional assessment of IL-17 production by ICS, regardless of the HIV infection status. However, both methods yield proportionate results with reduced absolute numbers of Th17 cells in untreated HIV disease, reflecting the depletion of total CD4+ T cells in viremic HIV patients, and restoration with cART. This article is protected by copyright. All rights reserved.
AB - INTRODUCTION: Th17 cells can either be identified by co-staining of surface markers or by intracellular cytokine staining (ICS) for IL-17 production. Discrepancies regarding the published frequencies of Th17 cells in peripheral blood mononuclear cells (PBMC) of HIV patients may partly be due to the different methodologies used.METHODS: Cryopreserved PBMCs from healthy controls and HIV infected subjects, including treated (cART) and viremic patients, were split and analyzed side by side by flow cytometry for expression of surface markers CCR6, CXCR3, CCR4 and CD161, or for intracellular expression of IL-17A and IFNγ after stimulation.RESULTS: The characterization of Th17 cells as CCR6+CXCR3-CCR4+CD161+ yielded considerably higher frequencies than the corresponding frequencies obtained by characterization via cytokines (IL-17+IFNγ-), regardless of the HIV status. However, the overall frequencies delivered by the two methods significantly correlated. The relative frequency of Th17 cells within the CD4+ T cell compartment was preserved in HIV infection, but there was a significant decrease in the absolute Th17 number, which was restored after initiation of cART, paralleling CD4+ T cell recovery. Absolute Th17 numbers inversely correlated with HIV viral load.CONCLUSION: The definition of Th17 cells by surface markers might overestimate their frequency in comparison to functional assessment of IL-17 production by ICS, regardless of the HIV infection status. However, both methods yield proportionate results with reduced absolute numbers of Th17 cells in untreated HIV disease, reflecting the depletion of total CD4+ T cells in viremic HIV patients, and restoration with cART. This article is protected by copyright. All rights reserved.
U2 - 10.1002/cyto.b.21352
DO - 10.1002/cyto.b.21352
M3 - SCORING: Journal article
C2 - 26666875
VL - 90
SP - 486
EP - 492
JO - CYTOM PART B-CLIN CY
JF - CYTOM PART B-CLIN CY
SN - 1552-4949
IS - 6
ER -