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Pancreatic carcinoma cell lines reflect frequency and variability of cancer stem cell markers in clinical tissue

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Pancreatic carcinoma cell lines reflect frequency and variability of cancer stem cell markers in clinical tissue. / Bünger, S; Barow, M; Thorns, C; Freitag-Wolf, S; Danner, S; Tiede, S; Pries, R; Görg, S; Bruch, H-P; Roblick, U J; Kruse, C; Habermann, J K.

In: EUR SURG RES, Vol. 49, No. 2, 2012, p. 88-98.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Bünger, S, Barow, M, Thorns, C, Freitag-Wolf, S, Danner, S, Tiede, S, Pries, R, Görg, S, Bruch, H-P, Roblick, UJ, Kruse, C & Habermann, JK 2012, 'Pancreatic carcinoma cell lines reflect frequency and variability of cancer stem cell markers in clinical tissue', EUR SURG RES, vol. 49, no. 2, pp. 88-98. https://doi.org/10.1159/000341669

APA

Bünger, S., Barow, M., Thorns, C., Freitag-Wolf, S., Danner, S., Tiede, S., Pries, R., Görg, S., Bruch, H-P., Roblick, U. J., Kruse, C., & Habermann, J. K. (2012). Pancreatic carcinoma cell lines reflect frequency and variability of cancer stem cell markers in clinical tissue. EUR SURG RES, 49(2), 88-98. https://doi.org/10.1159/000341669

Vancouver

Bünger S, Barow M, Thorns C, Freitag-Wolf S, Danner S, Tiede S et al. Pancreatic carcinoma cell lines reflect frequency and variability of cancer stem cell markers in clinical tissue. EUR SURG RES. 2012;49(2):88-98. https://doi.org/10.1159/000341669

Bibtex

@article{969c50b972054accaa90fea89aff9fc7,
title = "Pancreatic carcinoma cell lines reflect frequency and variability of cancer stem cell markers in clinical tissue",
abstract = "BACKGROUND: Pancreatic cancer is one of the most deadly malignancies with insufficient therapeutic options and poor outcome. Cancer stem cells (CSCs) are thought to be responsible for progression and therapy resistance. We investigated the potential of pancreatic cell lines for CSC research by analyzing to what extent they contain CSC populations and how representative these are compared to clinical tissue.METHODS: Six pancreatic cancer cell lines were analyzed by flow cytometry for CD326, CD133, CD44, CD24, CXCR4 and ABCG2. Subsequently, 70 primary pancreatic tissues were evaluated for CD326, CD133 and CD44 by immunohistochemistry.RESULTS: All the cell lines but one showed a stable expression pattern throughout biological replicates. Marker expression in clinical tissue of CD44 distinguished normal patients from pancreatic carcinoma patients with a sensitivity of 50% at 80% specificity and metastasized from nonmetastasized carcinomas with 69% sensitivity at 100% specificity.CONCLUSIONS: Our results indicate a link between elevated CD44 expression, malignancy and metastasis of pancreatic tissue. Furthermore, individual pancreatic cell lines show a substantial amount of cells with CSC properties which is comparable with interpatient variability detected in primary tissue. These pancreatic cancer cell lines could thus serve for urgently needed pharmacological CSC in vitro research.",
keywords = "Aged, Aged, 80 and over, Biomarkers, Tumor, Carcinoma, Case-Control Studies, Cell Line, Tumor, Female, Flow Cytometry, Humans, Male, Middle Aged, Neoplastic Stem Cells, Pancreas, Pancreatic Neoplasms, Comparative Study, Journal Article",
author = "S B{\"u}nger and M Barow and C Thorns and S Freitag-Wolf and S Danner and S Tiede and R Pries and S G{\"o}rg and H-P Bruch and Roblick, {U J} and C Kruse and Habermann, {J K}",
note = "Copyright {\textcopyright} 2012 S. Karger AG, Basel.",
year = "2012",
doi = "10.1159/000341669",
language = "English",
volume = "49",
pages = "88--98",
journal = "EUR SURG RES",
issn = "0014-312X",
publisher = "S. Karger AG",
number = "2",

}

RIS

TY - JOUR

T1 - Pancreatic carcinoma cell lines reflect frequency and variability of cancer stem cell markers in clinical tissue

AU - Bünger, S

AU - Barow, M

AU - Thorns, C

AU - Freitag-Wolf, S

AU - Danner, S

AU - Tiede, S

AU - Pries, R

AU - Görg, S

AU - Bruch, H-P

AU - Roblick, U J

AU - Kruse, C

AU - Habermann, J K

N1 - Copyright © 2012 S. Karger AG, Basel.

PY - 2012

Y1 - 2012

N2 - BACKGROUND: Pancreatic cancer is one of the most deadly malignancies with insufficient therapeutic options and poor outcome. Cancer stem cells (CSCs) are thought to be responsible for progression and therapy resistance. We investigated the potential of pancreatic cell lines for CSC research by analyzing to what extent they contain CSC populations and how representative these are compared to clinical tissue.METHODS: Six pancreatic cancer cell lines were analyzed by flow cytometry for CD326, CD133, CD44, CD24, CXCR4 and ABCG2. Subsequently, 70 primary pancreatic tissues were evaluated for CD326, CD133 and CD44 by immunohistochemistry.RESULTS: All the cell lines but one showed a stable expression pattern throughout biological replicates. Marker expression in clinical tissue of CD44 distinguished normal patients from pancreatic carcinoma patients with a sensitivity of 50% at 80% specificity and metastasized from nonmetastasized carcinomas with 69% sensitivity at 100% specificity.CONCLUSIONS: Our results indicate a link between elevated CD44 expression, malignancy and metastasis of pancreatic tissue. Furthermore, individual pancreatic cell lines show a substantial amount of cells with CSC properties which is comparable with interpatient variability detected in primary tissue. These pancreatic cancer cell lines could thus serve for urgently needed pharmacological CSC in vitro research.

AB - BACKGROUND: Pancreatic cancer is one of the most deadly malignancies with insufficient therapeutic options and poor outcome. Cancer stem cells (CSCs) are thought to be responsible for progression and therapy resistance. We investigated the potential of pancreatic cell lines for CSC research by analyzing to what extent they contain CSC populations and how representative these are compared to clinical tissue.METHODS: Six pancreatic cancer cell lines were analyzed by flow cytometry for CD326, CD133, CD44, CD24, CXCR4 and ABCG2. Subsequently, 70 primary pancreatic tissues were evaluated for CD326, CD133 and CD44 by immunohistochemistry.RESULTS: All the cell lines but one showed a stable expression pattern throughout biological replicates. Marker expression in clinical tissue of CD44 distinguished normal patients from pancreatic carcinoma patients with a sensitivity of 50% at 80% specificity and metastasized from nonmetastasized carcinomas with 69% sensitivity at 100% specificity.CONCLUSIONS: Our results indicate a link between elevated CD44 expression, malignancy and metastasis of pancreatic tissue. Furthermore, individual pancreatic cell lines show a substantial amount of cells with CSC properties which is comparable with interpatient variability detected in primary tissue. These pancreatic cancer cell lines could thus serve for urgently needed pharmacological CSC in vitro research.

KW - Aged

KW - Aged, 80 and over

KW - Biomarkers, Tumor

KW - Carcinoma

KW - Case-Control Studies

KW - Cell Line, Tumor

KW - Female

KW - Flow Cytometry

KW - Humans

KW - Male

KW - Middle Aged

KW - Neoplastic Stem Cells

KW - Pancreas

KW - Pancreatic Neoplasms

KW - Comparative Study

KW - Journal Article

U2 - 10.1159/000341669

DO - 10.1159/000341669

M3 - SCORING: Journal article

C2 - 22948659

VL - 49

SP - 88

EP - 98

JO - EUR SURG RES

JF - EUR SURG RES

SN - 0014-312X

IS - 2

ER -