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Oxidative stress increases synthesis of big endothelin-1 by activation of the endothelin-1 promoter

Standard

Oxidative stress increases synthesis of big endothelin-1 by activation of the endothelin-1 promoter. / Kähler, Jan; Mendel, Sabine; Weckmüller, Jörn; Orzechowski, Hans Dieter; Mittmann, Clemens; Köster, Ralf; Paul, Martin; Meinertz, Thomas; Münzel, Thomas.

In: J MOL CELL CARDIOL, Vol. 32, No. 8, 2000, p. 1429-1437.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Kähler, J, Mendel, S, Weckmüller, J, Orzechowski, HD, Mittmann, C, Köster, R, Paul, M, Meinertz, T & Münzel, T 2000, 'Oxidative stress increases synthesis of big endothelin-1 by activation of the endothelin-1 promoter', J MOL CELL CARDIOL, vol. 32, no. 8, pp. 1429-1437. https://doi.org/10.1006/jmcc.2000.1178

APA

Kähler, J., Mendel, S., Weckmüller, J., Orzechowski, H. D., Mittmann, C., Köster, R., Paul, M., Meinertz, T., & Münzel, T. (2000). Oxidative stress increases synthesis of big endothelin-1 by activation of the endothelin-1 promoter. J MOL CELL CARDIOL, 32(8), 1429-1437. https://doi.org/10.1006/jmcc.2000.1178

Vancouver

Kähler J, Mendel S, Weckmüller J, Orzechowski HD, Mittmann C, Köster R et al. Oxidative stress increases synthesis of big endothelin-1 by activation of the endothelin-1 promoter. J MOL CELL CARDIOL. 2000;32(8):1429-1437. https://doi.org/10.1006/jmcc.2000.1178

Bibtex

@article{6b6f663ca90044b2a24181579f8ff0b7,
title = "Oxidative stress increases synthesis of big endothelin-1 by activation of the endothelin-1 promoter",
abstract = "Modulation of the biosynthesis of the vasoconstrictor peptide endothelin-1 by oxygen-derived free radicals generated by xanthine oxidase or hydrogen peroxide was studied in cultured endothelial cells. Endothelin-1 metabolism was investigated at the level of endothelin-1 promoter, preproendothelin-1 mRNA and intracellular big endothelin-1. Endothelin-1 mRNA, as characterized by Northern blotting, was increased both time- and dose-dependently by xanthine oxidase to up to 500% above baseline. Analysis of endothelin-1 promoter activity using a construct containing 1329 bp of the endothelin-1 promoter revealed that promoter activity was increased up to eight-fold by incubation with xanthine oxidase. Specificity was ascertained by co-incubation with superoxide dismutase and catalase leading to inhibition of the effect of xanthine oxidase. A significant contribution of nitric oxide was ruled out, since NOS III-mRNA transcription remained unchanged and L-NAME did not significantly alter endothelin-1 promoter activity. Synthesis of intracellular big endothelin-1 protein was increased dose-dependently by xanthine oxidase. Our results indicate that oxidative stress leads to increased endothelial synthesis of big endothelin-1, which is a previously unknown mechanism and may help to understand the detrimental association of increased oxidative stress and elevated endothelin-1 levels in pathophysiological conditions promoting atherosclerosis. (C) 2000 Academic Press.",
keywords = "Endothelin-1, Oxygen-derived radicals, Preproendothelin-1 mRNA, Promoter",
author = "Jan K{\"a}hler and Sabine Mendel and J{\"o}rn Weckm{\"u}ller and Orzechowski, {Hans Dieter} and Clemens Mittmann and Ralf K{\"o}ster and Martin Paul and Thomas Meinertz and Thomas M{\"u}nzel",
year = "2000",
doi = "10.1006/jmcc.2000.1178",
language = "English",
volume = "32",
pages = "1429--1437",
journal = "J MOL CELL CARDIOL",
issn = "0022-2828",
publisher = "Academic Press Inc.",
number = "8",

}

RIS

TY - JOUR

T1 - Oxidative stress increases synthesis of big endothelin-1 by activation of the endothelin-1 promoter

AU - Kähler, Jan

AU - Mendel, Sabine

AU - Weckmüller, Jörn

AU - Orzechowski, Hans Dieter

AU - Mittmann, Clemens

AU - Köster, Ralf

AU - Paul, Martin

AU - Meinertz, Thomas

AU - Münzel, Thomas

PY - 2000

Y1 - 2000

N2 - Modulation of the biosynthesis of the vasoconstrictor peptide endothelin-1 by oxygen-derived free radicals generated by xanthine oxidase or hydrogen peroxide was studied in cultured endothelial cells. Endothelin-1 metabolism was investigated at the level of endothelin-1 promoter, preproendothelin-1 mRNA and intracellular big endothelin-1. Endothelin-1 mRNA, as characterized by Northern blotting, was increased both time- and dose-dependently by xanthine oxidase to up to 500% above baseline. Analysis of endothelin-1 promoter activity using a construct containing 1329 bp of the endothelin-1 promoter revealed that promoter activity was increased up to eight-fold by incubation with xanthine oxidase. Specificity was ascertained by co-incubation with superoxide dismutase and catalase leading to inhibition of the effect of xanthine oxidase. A significant contribution of nitric oxide was ruled out, since NOS III-mRNA transcription remained unchanged and L-NAME did not significantly alter endothelin-1 promoter activity. Synthesis of intracellular big endothelin-1 protein was increased dose-dependently by xanthine oxidase. Our results indicate that oxidative stress leads to increased endothelial synthesis of big endothelin-1, which is a previously unknown mechanism and may help to understand the detrimental association of increased oxidative stress and elevated endothelin-1 levels in pathophysiological conditions promoting atherosclerosis. (C) 2000 Academic Press.

AB - Modulation of the biosynthesis of the vasoconstrictor peptide endothelin-1 by oxygen-derived free radicals generated by xanthine oxidase or hydrogen peroxide was studied in cultured endothelial cells. Endothelin-1 metabolism was investigated at the level of endothelin-1 promoter, preproendothelin-1 mRNA and intracellular big endothelin-1. Endothelin-1 mRNA, as characterized by Northern blotting, was increased both time- and dose-dependently by xanthine oxidase to up to 500% above baseline. Analysis of endothelin-1 promoter activity using a construct containing 1329 bp of the endothelin-1 promoter revealed that promoter activity was increased up to eight-fold by incubation with xanthine oxidase. Specificity was ascertained by co-incubation with superoxide dismutase and catalase leading to inhibition of the effect of xanthine oxidase. A significant contribution of nitric oxide was ruled out, since NOS III-mRNA transcription remained unchanged and L-NAME did not significantly alter endothelin-1 promoter activity. Synthesis of intracellular big endothelin-1 protein was increased dose-dependently by xanthine oxidase. Our results indicate that oxidative stress leads to increased endothelial synthesis of big endothelin-1, which is a previously unknown mechanism and may help to understand the detrimental association of increased oxidative stress and elevated endothelin-1 levels in pathophysiological conditions promoting atherosclerosis. (C) 2000 Academic Press.

KW - Endothelin-1

KW - Oxygen-derived radicals

KW - Preproendothelin-1 mRNA

KW - Promoter

UR - http://www.scopus.com/inward/record.url?scp=0033858177&partnerID=8YFLogxK

U2 - 10.1006/jmcc.2000.1178

DO - 10.1006/jmcc.2000.1178

M3 - SCORING: Journal article

AN - SCOPUS:0033858177

VL - 32

SP - 1429

EP - 1437

JO - J MOL CELL CARDIOL

JF - J MOL CELL CARDIOL

SN - 0022-2828

IS - 8

ER -