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Overcoming chemoresistance in pancreatic cancer cells role of the bitter taste receptor T2R10

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Overcoming chemoresistance in pancreatic cancer cells role of the bitter taste receptor T2R10. / Stern, Louisa; Giese, Nathalia; Hackert, Thilo; Strobel, Oliver; Schirmacher, Peter; Felix, Klaus; Gaida, Matthias M.

In: J CANCER, Vol. 9, No. 4, 2018, p. 711-725.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Stern, L, Giese, N, Hackert, T, Strobel, O, Schirmacher, P, Felix, K & Gaida, MM 2018, 'Overcoming chemoresistance in pancreatic cancer cells role of the bitter taste receptor T2R10', J CANCER, vol. 9, no. 4, pp. 711-725. https://doi.org/10.7150/jca.21803

APA

Stern, L., Giese, N., Hackert, T., Strobel, O., Schirmacher, P., Felix, K., & Gaida, M. M. (2018). Overcoming chemoresistance in pancreatic cancer cells role of the bitter taste receptor T2R10. J CANCER, 9(4), 711-725. https://doi.org/10.7150/jca.21803

Vancouver

Stern L, Giese N, Hackert T, Strobel O, Schirmacher P, Felix K et al. Overcoming chemoresistance in pancreatic cancer cells role of the bitter taste receptor T2R10. J CANCER. 2018;9(4):711-725. https://doi.org/10.7150/jca.21803

Bibtex

@article{3e54cd1dd8fd40849e828468aed3769c,
title = "Overcoming chemoresistance in pancreatic cancer cells role of the bitter taste receptor T2R10",
abstract = "Bitter taste receptors (T2Rs) are G-protein coupled transmembrane proteins initially identified in the gustatory system as sensors for the taste of bitter. Recent evidence on expression of these receptors outside gustatory tissues suggested alternative functions, and there is growing interest of their potential role in cancer biology. In this study, we report for the first time, expression and functionality of the bitter receptor family member T2R10 in both human pancreatic ductal adenocarcinoma (PDAC) tissue and PDAC derived cell lines. Caffeine, a known ligand for T2R10, rendered the tumor cells more susceptible to two standard chemotherapeutics, Gemcitabine and 5-Fluoruracil. Knocking down T2R10 in the cell line BxPC-3 reduced the caffeine-induced effect. As possible underlying mechanism, we found that caffeine via triggering T2R10 inhibited Akt phosphorylation and subsequently downregulated expression of ABCG2, the so-called multi-drug resistance protein that participates in rendering cells resistant to a variety of chemotherapeutics. In conclusion, T2R10 is expressed in pancreatic cancer and it downmodulates the chemoresistance of the tumor cells.",
author = "Louisa Stern and Nathalia Giese and Thilo Hackert and Oliver Strobel and Peter Schirmacher and Klaus Felix and Gaida, {Matthias M}",
year = "2018",
doi = "10.7150/jca.21803",
language = "English",
volume = "9",
pages = "711--725",
journal = "J CANCER",
issn = "1837-9664",
publisher = "IVYSPRING INT PUBL ",
number = "4",

}

RIS

TY - JOUR

T1 - Overcoming chemoresistance in pancreatic cancer cells role of the bitter taste receptor T2R10

AU - Stern, Louisa

AU - Giese, Nathalia

AU - Hackert, Thilo

AU - Strobel, Oliver

AU - Schirmacher, Peter

AU - Felix, Klaus

AU - Gaida, Matthias M

PY - 2018

Y1 - 2018

N2 - Bitter taste receptors (T2Rs) are G-protein coupled transmembrane proteins initially identified in the gustatory system as sensors for the taste of bitter. Recent evidence on expression of these receptors outside gustatory tissues suggested alternative functions, and there is growing interest of their potential role in cancer biology. In this study, we report for the first time, expression and functionality of the bitter receptor family member T2R10 in both human pancreatic ductal adenocarcinoma (PDAC) tissue and PDAC derived cell lines. Caffeine, a known ligand for T2R10, rendered the tumor cells more susceptible to two standard chemotherapeutics, Gemcitabine and 5-Fluoruracil. Knocking down T2R10 in the cell line BxPC-3 reduced the caffeine-induced effect. As possible underlying mechanism, we found that caffeine via triggering T2R10 inhibited Akt phosphorylation and subsequently downregulated expression of ABCG2, the so-called multi-drug resistance protein that participates in rendering cells resistant to a variety of chemotherapeutics. In conclusion, T2R10 is expressed in pancreatic cancer and it downmodulates the chemoresistance of the tumor cells.

AB - Bitter taste receptors (T2Rs) are G-protein coupled transmembrane proteins initially identified in the gustatory system as sensors for the taste of bitter. Recent evidence on expression of these receptors outside gustatory tissues suggested alternative functions, and there is growing interest of their potential role in cancer biology. In this study, we report for the first time, expression and functionality of the bitter receptor family member T2R10 in both human pancreatic ductal adenocarcinoma (PDAC) tissue and PDAC derived cell lines. Caffeine, a known ligand for T2R10, rendered the tumor cells more susceptible to two standard chemotherapeutics, Gemcitabine and 5-Fluoruracil. Knocking down T2R10 in the cell line BxPC-3 reduced the caffeine-induced effect. As possible underlying mechanism, we found that caffeine via triggering T2R10 inhibited Akt phosphorylation and subsequently downregulated expression of ABCG2, the so-called multi-drug resistance protein that participates in rendering cells resistant to a variety of chemotherapeutics. In conclusion, T2R10 is expressed in pancreatic cancer and it downmodulates the chemoresistance of the tumor cells.

U2 - 10.7150/jca.21803

DO - 10.7150/jca.21803

M3 - SCORING: Journal article

C2 - 29556329

VL - 9

SP - 711

EP - 725

JO - J CANCER

JF - J CANCER

SN - 1837-9664

IS - 4

ER -