Research ExplorerPublicationsOral polio vaccination leads to oligoclonal expansion of TCR...

Oral polio vaccination leads to oligoclonal expansion of TCRBV16+ and TCRBV13+ T cells in the colon of rhesus macaques

Standard

Oral polio vaccination leads to oligoclonal expansion of TCRBV16+ and TCRBV13+ T cells in the colon of rhesus macaques. / Hörer, Sabine; Burdak-Rothkamm, Susanne; Allers, Kristina; Schneider, Thomas; Duchmann, Rainer.

In: EXP MOL PATHOL, Vol. 85, No. 3, 12.2008, p. 189-95.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Hörer, S, Burdak-Rothkamm, S, Allers, K, Schneider, T & Duchmann, R 2008, 'Oral polio vaccination leads to oligoclonal expansion of TCRBV16+ and TCRBV13+ T cells in the colon of rhesus macaques', EXP MOL PATHOL, vol. 85, no. 3, pp. 189-95. https://doi.org/10.1016/j.yexmp.2008.08.001

APA

Hörer, S., Burdak-Rothkamm, S., Allers, K., Schneider, T., & Duchmann, R. (2008). Oral polio vaccination leads to oligoclonal expansion of TCRBV16+ and TCRBV13+ T cells in the colon of rhesus macaques. EXP MOL PATHOL, 85(3), 189-95. https://doi.org/10.1016/j.yexmp.2008.08.001

Vancouver

Hörer S, Burdak-Rothkamm S, Allers K, Schneider T, Duchmann R. Oral polio vaccination leads to oligoclonal expansion of TCRBV16+ and TCRBV13+ T cells in the colon of rhesus macaques. EXP MOL PATHOL. 2008 Dec;85(3):189-95. https://doi.org/10.1016/j.yexmp.2008.08.001

Bibtex

@article{383fa944361f4d68831356bbd520675c,
title = "Oral polio vaccination leads to oligoclonal expansion of TCRBV16+ and TCRBV13+ T cells in the colon of rhesus macaques",
abstract = "In studying immune responses towards the poliovirus, data about T cell mediated immunity in the intestine as the main portal of viral entry in disease and vaccination is lacking. We treated two macaques with oral Polio vaccine and collected duodenal and colonic biopsy specimens. RNA isolation, reverse transcription, and polymerase chain reaction were performed for fragment analysis of the complementarity determining region 3 (CDR3) of the T cell receptor beta chain variable region (TCRBV), followed by subcloning and sequencing of expanded bands. In the colon, oligoclonal expansions of TCRBV16+ or TCRBV13+ intestinal T cells with conserved motifs of the hypervariable CDR3 were found. Flow cytometric analysis of mucosal T cells revealed that activated colonic T cells were mainly CD4(+). Our results indicate that there is a local activation of oligoclonal T cells in the colon after oral Polio vaccination (OPV) which involves selected TCRBV families and may occur within the CD4(+) T cell subset.",
keywords = "Amino Acid Motifs, Amino Acid Sequence, Animals, Antigens, Viral/immunology, CD4-Positive T-Lymphocytes/cytology, Cell Proliferation, Clone Cells, Colon/cytology, Complementarity Determining Regions/chemistry, Duodenum/cytology, Lymphocyte Activation/immunology, Macaca mulatta/immunology, Male, Molecular Sequence Data, Poliovirus Vaccine, Oral/immunology, Receptors, Antigen, T-Cell, alpha-beta/immunology, T-Lymphocytes/cytology",
author = "Sabine H{\"o}rer and Susanne Burdak-Rothkamm and Kristina Allers and Thomas Schneider and Rainer Duchmann",
year = "2008",
month = dec,
doi = "10.1016/j.yexmp.2008.08.001",
language = "English",
volume = "85",
pages = "189--95",
journal = "EXP MOL PATHOL",
issn = "0014-4800",
publisher = "Academic Press Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Oral polio vaccination leads to oligoclonal expansion of TCRBV16+ and TCRBV13+ T cells in the colon of rhesus macaques

AU - Hörer, Sabine

AU - Burdak-Rothkamm, Susanne

AU - Allers, Kristina

AU - Schneider, Thomas

AU - Duchmann, Rainer

PY - 2008/12

Y1 - 2008/12

N2 - In studying immune responses towards the poliovirus, data about T cell mediated immunity in the intestine as the main portal of viral entry in disease and vaccination is lacking. We treated two macaques with oral Polio vaccine and collected duodenal and colonic biopsy specimens. RNA isolation, reverse transcription, and polymerase chain reaction were performed for fragment analysis of the complementarity determining region 3 (CDR3) of the T cell receptor beta chain variable region (TCRBV), followed by subcloning and sequencing of expanded bands. In the colon, oligoclonal expansions of TCRBV16+ or TCRBV13+ intestinal T cells with conserved motifs of the hypervariable CDR3 were found. Flow cytometric analysis of mucosal T cells revealed that activated colonic T cells were mainly CD4(+). Our results indicate that there is a local activation of oligoclonal T cells in the colon after oral Polio vaccination (OPV) which involves selected TCRBV families and may occur within the CD4(+) T cell subset.

AB - In studying immune responses towards the poliovirus, data about T cell mediated immunity in the intestine as the main portal of viral entry in disease and vaccination is lacking. We treated two macaques with oral Polio vaccine and collected duodenal and colonic biopsy specimens. RNA isolation, reverse transcription, and polymerase chain reaction were performed for fragment analysis of the complementarity determining region 3 (CDR3) of the T cell receptor beta chain variable region (TCRBV), followed by subcloning and sequencing of expanded bands. In the colon, oligoclonal expansions of TCRBV16+ or TCRBV13+ intestinal T cells with conserved motifs of the hypervariable CDR3 were found. Flow cytometric analysis of mucosal T cells revealed that activated colonic T cells were mainly CD4(+). Our results indicate that there is a local activation of oligoclonal T cells in the colon after oral Polio vaccination (OPV) which involves selected TCRBV families and may occur within the CD4(+) T cell subset.

KW - Amino Acid Motifs

KW - Amino Acid Sequence

KW - Animals

KW - Antigens, Viral/immunology

KW - CD4-Positive T-Lymphocytes/cytology

KW - Cell Proliferation

KW - Clone Cells

KW - Colon/cytology

KW - Complementarity Determining Regions/chemistry

KW - Duodenum/cytology

KW - Lymphocyte Activation/immunology

KW - Macaca mulatta/immunology

KW - Male

KW - Molecular Sequence Data

KW - Poliovirus Vaccine, Oral/immunology

KW - Receptors, Antigen, T-Cell, alpha-beta/immunology

KW - T-Lymphocytes/cytology

U2 - 10.1016/j.yexmp.2008.08.001

DO - 10.1016/j.yexmp.2008.08.001

M3 - SCORING: Journal article

C2 - 18775423

VL - 85

SP - 189

EP - 195

JO - EXP MOL PATHOL

JF - EXP MOL PATHOL

SN - 0014-4800

IS - 3

ER -