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Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors

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Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors. / Zhou, Fangmin; Tichy, Alexandra-Madelaine; Imambocus, Bibi Nusreen; Sakharwade, Shreyas; Rodriguez Jimenez, Francisco J; González Martínez, Marco; Jahan, Ishrat; Habib, Margarita; Wilhelmy, Nina; Burre, Vanessa; Lömker, Tatjana; Sauter, Kathrin; Helfrich-Förster, Charlotte; Pielage, Jan; Grunwald Kadow, Ilona C; Janovjak, Harald; Soba, Peter.

In: NAT COMMUN, Vol. 14, No. 1, 19.12.2023, p. 8434.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Zhou, F, Tichy, A-M, Imambocus, BN, Sakharwade, S, Rodriguez Jimenez, FJ, González Martínez, M, Jahan, I, Habib, M, Wilhelmy, N, Burre, V, Lömker, T, Sauter, K, Helfrich-Förster, C, Pielage, J, Grunwald Kadow, IC, Janovjak, H & Soba, P 2023, 'Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors', NAT COMMUN, vol. 14, no. 1, pp. 8434. https://doi.org/10.1038/s41467-023-43970-0

APA

Zhou, F., Tichy, A-M., Imambocus, B. N., Sakharwade, S., Rodriguez Jimenez, F. J., González Martínez, M., Jahan, I., Habib, M., Wilhelmy, N., Burre, V., Lömker, T., Sauter, K., Helfrich-Förster, C., Pielage, J., Grunwald Kadow, I. C., Janovjak, H., & Soba, P. (2023). Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors. NAT COMMUN, 14(1), 8434. https://doi.org/10.1038/s41467-023-43970-0

Vancouver

Zhou F, Tichy A-M, Imambocus BN, Sakharwade S, Rodriguez Jimenez FJ, González Martínez M et al. Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors. NAT COMMUN. 2023 Dec 19;14(1):8434. https://doi.org/10.1038/s41467-023-43970-0

Bibtex

@article{4fdfa6cb3026478ba93d4354e690f990,
title = "Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors",
abstract = "Neuromodulatory signaling via G protein-coupled receptors (GPCRs) plays a pivotal role in regulating neural network function and animal behavior. The recent development of optogenetic tools to induce G protein-mediated signaling provides the promise of acute and cell type-specific manipulation of neuromodulatory signals. However, designing and deploying optogenetically functionalized GPCRs (optoXRs) with accurate specificity and activity to mimic endogenous signaling in vivo remains challenging. Here we optimize the design of optoXRs by considering evolutionary conserved GPCR-G protein interactions and demonstrate the feasibility of this approach using two Drosophila Dopamine receptors (optoDopRs). These optoDopRs exhibit high signaling specificity and light sensitivity in vitro. In vivo, we show receptor and cell type-specific effects of dopaminergic signaling in various behaviors, including the ability of optoDopRs to rescue the loss of the endogenous receptors. This work demonstrates that optoXRs can enable optical control of neuromodulatory receptor-specific signaling in functional and behavioral studies.",
keywords = "Animals, Receptors, Dopamine/genetics, Receptors, G-Protein-Coupled/metabolism, Signal Transduction, GTP-Binding Proteins/metabolism, Drosophila/genetics",
author = "Fangmin Zhou and Alexandra-Madelaine Tichy and Imambocus, {Bibi Nusreen} and Shreyas Sakharwade and {Rodriguez Jimenez}, {Francisco J} and {Gonz{\'a}lez Mart{\'i}nez}, Marco and Ishrat Jahan and Margarita Habib and Nina Wilhelmy and Vanessa Burre and Tatjana L{\"o}mker and Kathrin Sauter and Charlotte Helfrich-F{\"o}rster and Jan Pielage and {Grunwald Kadow}, {Ilona C} and Harald Janovjak and Peter Soba",
note = "{\textcopyright} 2023. The Author(s).",
year = "2023",
month = dec,
day = "19",
doi = "10.1038/s41467-023-43970-0",
language = "English",
volume = "14",
pages = "8434",
journal = "NAT COMMUN",
issn = "2041-1723",
publisher = "NATURE PUBLISHING GROUP",
number = "1",

}

RIS

TY - JOUR

T1 - Optimized design and in vivo application of optogenetically functionalized Drosophila dopamine receptors

AU - Zhou, Fangmin

AU - Tichy, Alexandra-Madelaine

AU - Imambocus, Bibi Nusreen

AU - Sakharwade, Shreyas

AU - Rodriguez Jimenez, Francisco J

AU - González Martínez, Marco

AU - Jahan, Ishrat

AU - Habib, Margarita

AU - Wilhelmy, Nina

AU - Burre, Vanessa

AU - Lömker, Tatjana

AU - Sauter, Kathrin

AU - Helfrich-Förster, Charlotte

AU - Pielage, Jan

AU - Grunwald Kadow, Ilona C

AU - Janovjak, Harald

AU - Soba, Peter

N1 - © 2023. The Author(s).

PY - 2023/12/19

Y1 - 2023/12/19

N2 - Neuromodulatory signaling via G protein-coupled receptors (GPCRs) plays a pivotal role in regulating neural network function and animal behavior. The recent development of optogenetic tools to induce G protein-mediated signaling provides the promise of acute and cell type-specific manipulation of neuromodulatory signals. However, designing and deploying optogenetically functionalized GPCRs (optoXRs) with accurate specificity and activity to mimic endogenous signaling in vivo remains challenging. Here we optimize the design of optoXRs by considering evolutionary conserved GPCR-G protein interactions and demonstrate the feasibility of this approach using two Drosophila Dopamine receptors (optoDopRs). These optoDopRs exhibit high signaling specificity and light sensitivity in vitro. In vivo, we show receptor and cell type-specific effects of dopaminergic signaling in various behaviors, including the ability of optoDopRs to rescue the loss of the endogenous receptors. This work demonstrates that optoXRs can enable optical control of neuromodulatory receptor-specific signaling in functional and behavioral studies.

AB - Neuromodulatory signaling via G protein-coupled receptors (GPCRs) plays a pivotal role in regulating neural network function and animal behavior. The recent development of optogenetic tools to induce G protein-mediated signaling provides the promise of acute and cell type-specific manipulation of neuromodulatory signals. However, designing and deploying optogenetically functionalized GPCRs (optoXRs) with accurate specificity and activity to mimic endogenous signaling in vivo remains challenging. Here we optimize the design of optoXRs by considering evolutionary conserved GPCR-G protein interactions and demonstrate the feasibility of this approach using two Drosophila Dopamine receptors (optoDopRs). These optoDopRs exhibit high signaling specificity and light sensitivity in vitro. In vivo, we show receptor and cell type-specific effects of dopaminergic signaling in various behaviors, including the ability of optoDopRs to rescue the loss of the endogenous receptors. This work demonstrates that optoXRs can enable optical control of neuromodulatory receptor-specific signaling in functional and behavioral studies.

KW - Animals

KW - Receptors, Dopamine/genetics

KW - Receptors, G-Protein-Coupled/metabolism

KW - Signal Transduction

KW - GTP-Binding Proteins/metabolism

KW - Drosophila/genetics

U2 - 10.1038/s41467-023-43970-0

DO - 10.1038/s41467-023-43970-0

M3 - SCORING: Journal article

C2 - 38114457

VL - 14

SP - 8434

JO - NAT COMMUN

JF - NAT COMMUN

SN - 2041-1723

IS - 1

ER -