Optimization of magnetic nanoparticles for engineering erythrocytes as theranostic agents

TY - JOUR

T1 - Optimization of magnetic nanoparticles for engineering erythrocytes as theranostic agents

AU - Slavu, Laura Maria

AU - Antonelli, Antonella

AU - Scarpa, Emanuele Salvatore

AU - Abdalla, Pasant

AU - Wilhelm, Claire

AU - Silvestri, Niccolò

AU - Pellegrino, Teresa

AU - Scheffler, Konrad

AU - Magnani, Mauro

AU - Rinaldi, Rosaria

AU - Di Corato, Riccardo

PY - 2023/5/2

Y1 - 2023/5/2

N2 - The application of superparamagnetic iron oxide nanoparticles (SPIONs) in drug delivery, magnetic resonance imaging, cell tracking, and hyperthermia has been long exploited regarding their inducible magnetic properties. Nevertheless, SPIONs remain rapidly cleared from the circulation by the reticuloendothelial system (RES) or mononuclear phagocyte system, with uptake dependent on several factors such as the hydrodynamic diameter, electrical charge and surface coating. This rapid clearance of SPION-based theranostic agents from circulation is one of the main challenges hampering the medical applications that differ from RES targeting. This work proposes a strategy to render biocompatible SPIONs through their encapsulation in the red blood cells (RBCs). In this work, the research has been focused on the multi-step optimization of chemical synthesis of magnetic nanoparticles (MNPs), precisely iron oxide nanoparticles (IONPs) and zinc manganese-ferrite nanoparticles (Zn/Mn FNPs), for encapsulation in human and murine RBCs. The encapsulation through the transient opening of RBC membrane pores requires extensive efforts to deliver high-quality nanoparticles in terms of chemical properties, morphology, stability and biocompatibility. After reaching this goal, in vitro experiments were performed with selected nanomaterials to investigate the potential of engineered MNP-RBC constructs in theranostic approaches.

AB - The application of superparamagnetic iron oxide nanoparticles (SPIONs) in drug delivery, magnetic resonance imaging, cell tracking, and hyperthermia has been long exploited regarding their inducible magnetic properties. Nevertheless, SPIONs remain rapidly cleared from the circulation by the reticuloendothelial system (RES) or mononuclear phagocyte system, with uptake dependent on several factors such as the hydrodynamic diameter, electrical charge and surface coating. This rapid clearance of SPION-based theranostic agents from circulation is one of the main challenges hampering the medical applications that differ from RES targeting. This work proposes a strategy to render biocompatible SPIONs through their encapsulation in the red blood cells (RBCs). In this work, the research has been focused on the multi-step optimization of chemical synthesis of magnetic nanoparticles (MNPs), precisely iron oxide nanoparticles (IONPs) and zinc manganese-ferrite nanoparticles (Zn/Mn FNPs), for encapsulation in human and murine RBCs. The encapsulation through the transient opening of RBC membrane pores requires extensive efforts to deliver high-quality nanoparticles in terms of chemical properties, morphology, stability and biocompatibility. After reaching this goal, in vitro experiments were performed with selected nanomaterials to investigate the potential of engineered MNP-RBC constructs in theranostic approaches.

KW - Mice

KW - Animals

KW - Humans

KW - Magnetite Nanoparticles/chemistry

KW - Precision Medicine

KW - Magnetic Resonance Imaging/methods

KW - Drug Delivery Systems

KW - Erythrocytes/metabolism

KW - Theranostic Nanomedicine/methods

U2 - 10.1039/d3bm00264k

DO - 10.1039/d3bm00264k

M3 - SCORING: Journal article

C2 - 36939172

VL - 11

SP - 3252

EP - 3268

JO - BIOMATER SCI-UK

JF - BIOMATER SCI-UK

SN - 2047-4830

IS - 9

ER -