One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system

Rajeev Raman; Anna Karpova; Michael R Kreutz

doi:10.1016/j.pep.2022.106057

One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system

Standard

One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system. / Raman, Rajeev; Karpova, Anna; Kreutz, Michael R.

In: PROTEIN EXPRES PURIF, Vol. 193, 106057, 05.2022.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Raman, R, Karpova, A & Kreutz, MR 2022, 'One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system', PROTEIN EXPRES PURIF, vol. 193, 106057. https://doi.org/10.1016/j.pep.2022.106057

APA

Raman, R., Karpova, A., & Kreutz, M. R. (2022). One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system. PROTEIN EXPRES PURIF, 193, [106057]. https://doi.org/10.1016/j.pep.2022.106057

Vancouver

Raman R, Karpova A, Kreutz MR. One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system. PROTEIN EXPRES PURIF. 2022 May;193. 106057. https://doi.org/10.1016/j.pep.2022.106057

Bibtex

@article{d5e61f9e798b4e0883899f3466e4d0f9,

title = "One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system",

abstract = "Lamin B1 is an intermediate filament protein that is a core component of the nuclear lamina. Structural studies and biochemical characterization of lamin B1 are severely hampered by the tendency of the protein to form inclusion bodies in E. coli bacterial expression systems. Therefore, the purity and consistency of the protein varies from batch to batch. In this work, we have purified a tag-free lamin B1 protein from a soluble fraction following bacterial expression. We also checked the functional properties of the purified as well as of the subsequently lyophilised protein. The current protocol helps to purify functional lamin B1 in a single step.",

author = "Rajeev Raman and Anna Karpova and Kreutz, {Michael R}",

note = "Copyright {\textcopyright} 2022. Published by Elsevier Inc.",

year = "2022",

month = may,

doi = "10.1016/j.pep.2022.106057",

language = "English",

volume = "193",

journal = "PROTEIN EXPRES PURIF",

issn = "1046-5928",

publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - One-step purification of tag free and soluble lamin B1 from an E. coli bacterial expression system

AU - Raman, Rajeev

AU - Karpova, Anna

AU - Kreutz, Michael R

PY - 2022/5

Y1 - 2022/5

N2 - Lamin B1 is an intermediate filament protein that is a core component of the nuclear lamina. Structural studies and biochemical characterization of lamin B1 are severely hampered by the tendency of the protein to form inclusion bodies in E. coli bacterial expression systems. Therefore, the purity and consistency of the protein varies from batch to batch. In this work, we have purified a tag-free lamin B1 protein from a soluble fraction following bacterial expression. We also checked the functional properties of the purified as well as of the subsequently lyophilised protein. The current protocol helps to purify functional lamin B1 in a single step.

AB - Lamin B1 is an intermediate filament protein that is a core component of the nuclear lamina. Structural studies and biochemical characterization of lamin B1 are severely hampered by the tendency of the protein to form inclusion bodies in E. coli bacterial expression systems. Therefore, the purity and consistency of the protein varies from batch to batch. In this work, we have purified a tag-free lamin B1 protein from a soluble fraction following bacterial expression. We also checked the functional properties of the purified as well as of the subsequently lyophilised protein. The current protocol helps to purify functional lamin B1 in a single step.

U2 - 10.1016/j.pep.2022.106057

DO - 10.1016/j.pep.2022.106057

M3 - SCORING: Journal article

C2 - 35077781

VL - 193

JO - PROTEIN EXPRES PURIF

JF - PROTEIN EXPRES PURIF

SN - 1046-5928

M1 - 106057

ER -