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OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells

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OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells. / Barros-Martins, Joana; Bruni, Elena; Fichtner, Alina Suzann; Cornberg, Markus; Prinz, Immo.

In: CYTOM PART A, Vol. 101, No. 10, 10.2022, p. 856-861.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Barros-Martins, J, Bruni, E, Fichtner, AS, Cornberg, M & Prinz, I 2022, 'OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells', CYTOM PART A, vol. 101, no. 10, pp. 856-861. https://doi.org/10.1002/cyto.a.24564

APA

Barros-Martins, J., Bruni, E., Fichtner, A. S., Cornberg, M., & Prinz, I. (2022). OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells. CYTOM PART A, 101(10), 856-861. https://doi.org/10.1002/cyto.a.24564

Vancouver

Barros-Martins J, Bruni E, Fichtner AS, Cornberg M, Prinz I. OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells. CYTOM PART A. 2022 Oct;101(10):856-861. https://doi.org/10.1002/cyto.a.24564

Bibtex

@article{360a4afd567a494a9cb3d2ae69848d50,
title = "OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells",
abstract = "Using full spectrum flow cytometry, we designed a 28-color panel for the analysis of markers known to be associated with the γδ T cell immune response. This panel allows the classification of γδ T cell subsets via specific V gene usage (Vγ9, Vδ1, Vδ2, and Vδ3) of their T cell receptor (TCR) and according to their functional differentiation. Phenotypical surface receptors to distinguish different stages of cell maturation included CD45RA, CD27, CD28, CD127, CD57, and CD16; chemokine receptors CXCR6, CCR5, CCR6, and CX3CR1; NK-associated markers NKG2A, NKG2D, CD56, and CD161, checkpoint-inhibitor PD-1, and activating receptors CD38 and CD25. T cell lineage markers for the analysis of αβ T cells (CD4 and CD8) and MAIT cells (Vα7.2) were also included. This optimized multicolor panel allows a comprehensive immune-profiling of all main human γδ T cell subsets and is suitable for longitudinal or exploratory analysis of γδ T cell development and γδ T cell dynamics in clinical cohorts.",
author = "Joana Barros-Martins and Elena Bruni and Fichtner, {Alina Suzann} and Markus Cornberg and Immo Prinz",
note = "{\textcopyright} 2022 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry.",
year = "2022",
month = oct,
doi = "10.1002/cyto.a.24564",
language = "English",
volume = "101",
pages = "856--861",
journal = "CYTOM PART A",
issn = "1552-4922",
publisher = "Wiley-Liss Inc.",
number = "10",

}

RIS

TY - JOUR

T1 - OMIP-084: 28-color full spectrum flow cytometry panel for the comprehensive analysis of human γδ T cells

AU - Barros-Martins, Joana

AU - Bruni, Elena

AU - Fichtner, Alina Suzann

AU - Cornberg, Markus

AU - Prinz, Immo

N1 - © 2022 The Authors. Cytometry Part A published by Wiley Periodicals LLC on behalf of International Society for Advancement of Cytometry.

PY - 2022/10

Y1 - 2022/10

N2 - Using full spectrum flow cytometry, we designed a 28-color panel for the analysis of markers known to be associated with the γδ T cell immune response. This panel allows the classification of γδ T cell subsets via specific V gene usage (Vγ9, Vδ1, Vδ2, and Vδ3) of their T cell receptor (TCR) and according to their functional differentiation. Phenotypical surface receptors to distinguish different stages of cell maturation included CD45RA, CD27, CD28, CD127, CD57, and CD16; chemokine receptors CXCR6, CCR5, CCR6, and CX3CR1; NK-associated markers NKG2A, NKG2D, CD56, and CD161, checkpoint-inhibitor PD-1, and activating receptors CD38 and CD25. T cell lineage markers for the analysis of αβ T cells (CD4 and CD8) and MAIT cells (Vα7.2) were also included. This optimized multicolor panel allows a comprehensive immune-profiling of all main human γδ T cell subsets and is suitable for longitudinal or exploratory analysis of γδ T cell development and γδ T cell dynamics in clinical cohorts.

AB - Using full spectrum flow cytometry, we designed a 28-color panel for the analysis of markers known to be associated with the γδ T cell immune response. This panel allows the classification of γδ T cell subsets via specific V gene usage (Vγ9, Vδ1, Vδ2, and Vδ3) of their T cell receptor (TCR) and according to their functional differentiation. Phenotypical surface receptors to distinguish different stages of cell maturation included CD45RA, CD27, CD28, CD127, CD57, and CD16; chemokine receptors CXCR6, CCR5, CCR6, and CX3CR1; NK-associated markers NKG2A, NKG2D, CD56, and CD161, checkpoint-inhibitor PD-1, and activating receptors CD38 and CD25. T cell lineage markers for the analysis of αβ T cells (CD4 and CD8) and MAIT cells (Vα7.2) were also included. This optimized multicolor panel allows a comprehensive immune-profiling of all main human γδ T cell subsets and is suitable for longitudinal or exploratory analysis of γδ T cell development and γδ T cell dynamics in clinical cohorts.

U2 - 10.1002/cyto.a.24564

DO - 10.1002/cyto.a.24564

M3 - SCORING: Journal article

C2 - 35521651

VL - 101

SP - 856

EP - 861

JO - CYTOM PART A

JF - CYTOM PART A

SN - 1552-4922

IS - 10

ER -