Research ExplorerPublicationsNovel Technology for Enrichment of Biomolecules from Cell-Fr...

Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing

Standard

Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing. / Patel, Vipulkumar; Celec, Peter; Grunt, Magdalena; Schwarzenbach, Heidi; Jenneckens, Ingo; Hillebrand, Timo.

In: ADV EXP MED BIOL, Vol. 924, 2016, p. 165-169.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Patel, V, Celec, P, Grunt, M, Schwarzenbach, H, Jenneckens, I & Hillebrand, T 2016, 'Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing', ADV EXP MED BIOL, vol. 924, pp. 165-169. https://doi.org/10.1007/978-3-319-42044-8_30

APA

Patel, V., Celec, P., Grunt, M., Schwarzenbach, H., Jenneckens, I., & Hillebrand, T. (2016). Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing. ADV EXP MED BIOL, 924, 165-169. https://doi.org/10.1007/978-3-319-42044-8_30

Vancouver

Patel V, Celec P, Grunt M, Schwarzenbach H, Jenneckens I, Hillebrand T. Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing. ADV EXP MED BIOL. 2016;924:165-169. https://doi.org/10.1007/978-3-319-42044-8_30

Bibtex

@article{a1aa499afa0f4b909137143a3a50aba4,
title = "Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing",
abstract = "Circulating cell-free DNA (ccfDNA) is a promising diagnostic tool and its size fractionation is of interest. However, kits for isolation of ccfDNA available on the market are designed for small volumes hence processing large sample volumes is laborious. We have tested a new method that enables enrichment of ccfDNA from large volumes of plasma and subsequently allows size-fractionation of isolated ccfDNA into two fractions with individually established cut-off levels of ccfDNA length. This method allows isolation of low-abundant DNA as well as separation of long and short DNA molecules. This procedure may be important e.g., in prenatal diagnostics and cancer research that have been already confirmed by our primary experiments. Here, we report the results of selective separation of 200- and 500-bp long synthetic DNA fragments spiked in plasma samples. Furthermore, we size-fractionated ccfDNA from the plasma of pregnant women and verified the prevalence of fetal ccfDNA in all fractions.",
author = "Vipulkumar Patel and Peter Celec and Magdalena Grunt and Heidi Schwarzenbach and Ingo Jenneckens and Timo Hillebrand",
year = "2016",
doi = "10.1007/978-3-319-42044-8_30",
language = "English",
volume = "924",
pages = "165--169",
journal = "ADV EXP MED BIOL",
issn = "0065-2598",
publisher = "Springer New York",

}

RIS

TY - JOUR

T1 - Novel Technology for Enrichment of Biomolecules from Cell-Free Body Fluids and Subsequent DNA Sizing

AU - Patel, Vipulkumar

AU - Celec, Peter

AU - Grunt, Magdalena

AU - Schwarzenbach, Heidi

AU - Jenneckens, Ingo

AU - Hillebrand, Timo

PY - 2016

Y1 - 2016

N2 - Circulating cell-free DNA (ccfDNA) is a promising diagnostic tool and its size fractionation is of interest. However, kits for isolation of ccfDNA available on the market are designed for small volumes hence processing large sample volumes is laborious. We have tested a new method that enables enrichment of ccfDNA from large volumes of plasma and subsequently allows size-fractionation of isolated ccfDNA into two fractions with individually established cut-off levels of ccfDNA length. This method allows isolation of low-abundant DNA as well as separation of long and short DNA molecules. This procedure may be important e.g., in prenatal diagnostics and cancer research that have been already confirmed by our primary experiments. Here, we report the results of selective separation of 200- and 500-bp long synthetic DNA fragments spiked in plasma samples. Furthermore, we size-fractionated ccfDNA from the plasma of pregnant women and verified the prevalence of fetal ccfDNA in all fractions.

AB - Circulating cell-free DNA (ccfDNA) is a promising diagnostic tool and its size fractionation is of interest. However, kits for isolation of ccfDNA available on the market are designed for small volumes hence processing large sample volumes is laborious. We have tested a new method that enables enrichment of ccfDNA from large volumes of plasma and subsequently allows size-fractionation of isolated ccfDNA into two fractions with individually established cut-off levels of ccfDNA length. This method allows isolation of low-abundant DNA as well as separation of long and short DNA molecules. This procedure may be important e.g., in prenatal diagnostics and cancer research that have been already confirmed by our primary experiments. Here, we report the results of selective separation of 200- and 500-bp long synthetic DNA fragments spiked in plasma samples. Furthermore, we size-fractionated ccfDNA from the plasma of pregnant women and verified the prevalence of fetal ccfDNA in all fractions.

U2 - 10.1007/978-3-319-42044-8_30

DO - 10.1007/978-3-319-42044-8_30

M3 - SCORING: Journal article

C2 - 27753038

VL - 924

SP - 165

EP - 169

JO - ADV EXP MED BIOL

JF - ADV EXP MED BIOL

SN - 0065-2598

ER -