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Novel DNA Methylation Sites Influence Expression in Relation to Smoking

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Novel DNA Methylation Sites Influence Expression in Relation to Smoking. / Haase, Tina; Müller, Christian; Krause, Julia; Röthemeier, Caroline; Stenzig, Justus; Kunze, Sonja; Waldenberger, Melanie; Münzel, Thomas; Pfeiffer, Norbert; Wild, Philipp S; Michal, Matthias; Marini, Federico; Karakas, Mahir; Lackner, Karl J; Blankenberg, Stefan; Zeller, Tanja.

In: BIOMOLECULES, Vol. 8, No. 3, 20.08.2018, p. E74.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Haase, T, Müller, C, Krause, J, Röthemeier, C, Stenzig, J, Kunze, S, Waldenberger, M, Münzel, T, Pfeiffer, N, Wild, PS, Michal, M, Marini, F, Karakas, M, Lackner, KJ, Blankenberg, S & Zeller, T 2018, 'Novel DNA Methylation Sites Influence Expression in Relation to Smoking', BIOMOLECULES, vol. 8, no. 3, pp. E74. https://doi.org/10.3390/biom8030074

APA

Haase, T., Müller, C., Krause, J., Röthemeier, C., Stenzig, J., Kunze, S., Waldenberger, M., Münzel, T., Pfeiffer, N., Wild, P. S., Michal, M., Marini, F., Karakas, M., Lackner, K. J., Blankenberg, S., & Zeller, T. (2018). Novel DNA Methylation Sites Influence Expression in Relation to Smoking. BIOMOLECULES, 8(3), E74. https://doi.org/10.3390/biom8030074

Vancouver

Haase T, Müller C, Krause J, Röthemeier C, Stenzig J, Kunze S et al. Novel DNA Methylation Sites Influence Expression in Relation to Smoking. BIOMOLECULES. 2018 Aug 20;8(3):E74. https://doi.org/10.3390/biom8030074

Bibtex

@article{da00ee5056b64f829f8d9ee9c5483ba1,
title = "Novel DNA Methylation Sites Influence Expression in Relation to Smoking",
abstract = "Smoking is a major risk factor for cardiovascular diseases and has been implicated in the regulation of the G protein-coupled receptor 15 (GPR15) by affecting CpG methylation. The G protein-coupled receptor 15 is involved in angiogenesis and inflammation. An effect on GPR15 gene regulation has been shown for the CpG site CpG3.98251294. We aimed to analyze the effect of smoking on GPR15 expression and methylation sites spanning the GPR15 locus. DNA methylation of nine GPR15 CpG sites was measured in leukocytes from 1291 population-based individuals using the EpiTYPER. Monocytic GPR15 expression was measured by qPCR at baseline and five-years follow up. GPR15 gene expression was upregulated in smokers (beta ({\ss}) = -2.699, p-value (p) = 1.02 × 10-77) and strongly correlated with smoking exposure ({\ss} = -0.063, p = 2.95 × 10-34). Smoking cessation within five years reduced GPR15 expression about 19% (p = 9.65 × 10-5) with decreasing GPR15 expression over time ({\ss} = 0.031, p = 3.81 × 10-6). Additionally, three novel CpG sites within GPR15 affected by smoking were identified. For CpG3.98251047, DNA methylation increased steadily after smoking cessation ({\ss} = 0.123, p = 1.67 × 10-3) and strongly correlated with changes in GPR15 expression ({\ss} = 0.036, p = 4.86 × 10-5). Three novel GPR15 CpG sites were identified in relation to smoking and GPR15 expression. Our results provide novel insights in the regulation of GPR15, which possibly linked smoking to inflammation and disease progression.",
keywords = "Journal Article",
author = "Tina Haase and Christian M{\"u}ller and Julia Krause and Caroline R{\"o}themeier and Justus Stenzig and Sonja Kunze and Melanie Waldenberger and Thomas M{\"u}nzel and Norbert Pfeiffer and Wild, {Philipp S} and Matthias Michal and Federico Marini and Mahir Karakas and Lackner, {Karl J} and Stefan Blankenberg and Tanja Zeller",
year = "2018",
month = aug,
day = "20",
doi = "10.3390/biom8030074",
language = "English",
volume = "8",
pages = "E74",
journal = "BIOMOLECULES",
issn = "2218-273X",
publisher = "Multidisciplinary Digital Publishing Institute",
number = "3",

}

RIS

TY - JOUR

T1 - Novel DNA Methylation Sites Influence Expression in Relation to Smoking

AU - Haase, Tina

AU - Müller, Christian

AU - Krause, Julia

AU - Röthemeier, Caroline

AU - Stenzig, Justus

AU - Kunze, Sonja

AU - Waldenberger, Melanie

AU - Münzel, Thomas

AU - Pfeiffer, Norbert

AU - Wild, Philipp S

AU - Michal, Matthias

AU - Marini, Federico

AU - Karakas, Mahir

AU - Lackner, Karl J

AU - Blankenberg, Stefan

AU - Zeller, Tanja

PY - 2018/8/20

Y1 - 2018/8/20

N2 - Smoking is a major risk factor for cardiovascular diseases and has been implicated in the regulation of the G protein-coupled receptor 15 (GPR15) by affecting CpG methylation. The G protein-coupled receptor 15 is involved in angiogenesis and inflammation. An effect on GPR15 gene regulation has been shown for the CpG site CpG3.98251294. We aimed to analyze the effect of smoking on GPR15 expression and methylation sites spanning the GPR15 locus. DNA methylation of nine GPR15 CpG sites was measured in leukocytes from 1291 population-based individuals using the EpiTYPER. Monocytic GPR15 expression was measured by qPCR at baseline and five-years follow up. GPR15 gene expression was upregulated in smokers (beta (ß) = -2.699, p-value (p) = 1.02 × 10-77) and strongly correlated with smoking exposure (ß = -0.063, p = 2.95 × 10-34). Smoking cessation within five years reduced GPR15 expression about 19% (p = 9.65 × 10-5) with decreasing GPR15 expression over time (ß = 0.031, p = 3.81 × 10-6). Additionally, three novel CpG sites within GPR15 affected by smoking were identified. For CpG3.98251047, DNA methylation increased steadily after smoking cessation (ß = 0.123, p = 1.67 × 10-3) and strongly correlated with changes in GPR15 expression (ß = 0.036, p = 4.86 × 10-5). Three novel GPR15 CpG sites were identified in relation to smoking and GPR15 expression. Our results provide novel insights in the regulation of GPR15, which possibly linked smoking to inflammation and disease progression.

AB - Smoking is a major risk factor for cardiovascular diseases and has been implicated in the regulation of the G protein-coupled receptor 15 (GPR15) by affecting CpG methylation. The G protein-coupled receptor 15 is involved in angiogenesis and inflammation. An effect on GPR15 gene regulation has been shown for the CpG site CpG3.98251294. We aimed to analyze the effect of smoking on GPR15 expression and methylation sites spanning the GPR15 locus. DNA methylation of nine GPR15 CpG sites was measured in leukocytes from 1291 population-based individuals using the EpiTYPER. Monocytic GPR15 expression was measured by qPCR at baseline and five-years follow up. GPR15 gene expression was upregulated in smokers (beta (ß) = -2.699, p-value (p) = 1.02 × 10-77) and strongly correlated with smoking exposure (ß = -0.063, p = 2.95 × 10-34). Smoking cessation within five years reduced GPR15 expression about 19% (p = 9.65 × 10-5) with decreasing GPR15 expression over time (ß = 0.031, p = 3.81 × 10-6). Additionally, three novel CpG sites within GPR15 affected by smoking were identified. For CpG3.98251047, DNA methylation increased steadily after smoking cessation (ß = 0.123, p = 1.67 × 10-3) and strongly correlated with changes in GPR15 expression (ß = 0.036, p = 4.86 × 10-5). Three novel GPR15 CpG sites were identified in relation to smoking and GPR15 expression. Our results provide novel insights in the regulation of GPR15, which possibly linked smoking to inflammation and disease progression.

KW - Journal Article

U2 - 10.3390/biom8030074

DO - 10.3390/biom8030074

M3 - SCORING: Journal article

C2 - 30127295

VL - 8

SP - E74

JO - BIOMOLECULES

JF - BIOMOLECULES

SN - 2218-273X

IS - 3

ER -