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Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster

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Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster. / Lange, Ulrike C; Adams, David J; Lee, Caroline; Barton, Sheila; Schneider, Robert; Bradley, Allan; Surani, M Azim.

In: MOL CELL BIOL, Vol. 28, No. 15, 01.08.2008, p. 4688-96.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Lange, UC, Adams, DJ, Lee, C, Barton, S, Schneider, R, Bradley, A & Surani, MA 2008, 'Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster', MOL CELL BIOL, vol. 28, no. 15, pp. 4688-96. https://doi.org/10.1128/MCB.00272-08

APA

Lange, U. C., Adams, D. J., Lee, C., Barton, S., Schneider, R., Bradley, A., & Surani, M. A. (2008). Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster. MOL CELL BIOL, 28(15), 4688-96. https://doi.org/10.1128/MCB.00272-08

Vancouver

Lange UC, Adams DJ, Lee C, Barton S, Schneider R, Bradley A et al. Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster. MOL CELL BIOL. 2008 Aug 1;28(15):4688-96. https://doi.org/10.1128/MCB.00272-08

Bibtex

@article{4a173246e7274fa3a3bd4688a38d1c9e,
title = "Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster",
abstract = "The family of interferon-inducible transmembrane proteins (Ifitm) consists of five highly sequence-related cell surface proteins, which are implicated in diverse cellular processes. Ifitm genes are conserved, widely expressed, and characteristically found in genomic clusters, such as the 67-kb Ifitm family locus on mouse chromosome 7. Recently, Ifitm1 and Ifitm3 have been suggested to mediate migration of early primordial germ cells (PGCs), a process that is little understood. To investigate Ifitm function during germ cell development, we used targeted chromosome engineering to generate mutants which either lack the entire Ifitm locus or carry a disrupted Ifitm3 gene only. Here we show that the mutations have no detectable effects on development of the germ line or on the generation of live young. Hence, contrary to previous reports, Ifitm genes are not essential for PGC migration. The Ifitm family is a striking example of a conserved gene cluster which appears to be functionally redundant during development.",
keywords = "Animals, Cell Lineage, Cell Movement, Embryo, Mammalian, Fertility, Gene Deletion, Gene Expression Regulation, Developmental, Gene Targeting, Germ Cells, Gonads, Green Fluorescent Proteins, Homozygote, Membrane Proteins, Mice, Multigene Family",
author = "Lange, {Ulrike C} and Adams, {David J} and Caroline Lee and Sheila Barton and Robert Schneider and Allan Bradley and Surani, {M Azim}",
year = "2008",
month = aug,
day = "1",
doi = "10.1128/MCB.00272-08",
language = "English",
volume = "28",
pages = "4688--96",
journal = "MOL CELL BIOL",
issn = "0270-7306",
publisher = "American Society for Microbiology",
number = "15",

}

RIS

TY - JOUR

T1 - Normal germ line establishment in mice carrying a deletion of the Ifitm/Fragilis gene family cluster

AU - Lange, Ulrike C

AU - Adams, David J

AU - Lee, Caroline

AU - Barton, Sheila

AU - Schneider, Robert

AU - Bradley, Allan

AU - Surani, M Azim

PY - 2008/8/1

Y1 - 2008/8/1

N2 - The family of interferon-inducible transmembrane proteins (Ifitm) consists of five highly sequence-related cell surface proteins, which are implicated in diverse cellular processes. Ifitm genes are conserved, widely expressed, and characteristically found in genomic clusters, such as the 67-kb Ifitm family locus on mouse chromosome 7. Recently, Ifitm1 and Ifitm3 have been suggested to mediate migration of early primordial germ cells (PGCs), a process that is little understood. To investigate Ifitm function during germ cell development, we used targeted chromosome engineering to generate mutants which either lack the entire Ifitm locus or carry a disrupted Ifitm3 gene only. Here we show that the mutations have no detectable effects on development of the germ line or on the generation of live young. Hence, contrary to previous reports, Ifitm genes are not essential for PGC migration. The Ifitm family is a striking example of a conserved gene cluster which appears to be functionally redundant during development.

AB - The family of interferon-inducible transmembrane proteins (Ifitm) consists of five highly sequence-related cell surface proteins, which are implicated in diverse cellular processes. Ifitm genes are conserved, widely expressed, and characteristically found in genomic clusters, such as the 67-kb Ifitm family locus on mouse chromosome 7. Recently, Ifitm1 and Ifitm3 have been suggested to mediate migration of early primordial germ cells (PGCs), a process that is little understood. To investigate Ifitm function during germ cell development, we used targeted chromosome engineering to generate mutants which either lack the entire Ifitm locus or carry a disrupted Ifitm3 gene only. Here we show that the mutations have no detectable effects on development of the germ line or on the generation of live young. Hence, contrary to previous reports, Ifitm genes are not essential for PGC migration. The Ifitm family is a striking example of a conserved gene cluster which appears to be functionally redundant during development.

KW - Animals

KW - Cell Lineage

KW - Cell Movement

KW - Embryo, Mammalian

KW - Fertility

KW - Gene Deletion

KW - Gene Expression Regulation, Developmental

KW - Gene Targeting

KW - Germ Cells

KW - Gonads

KW - Green Fluorescent Proteins

KW - Homozygote

KW - Membrane Proteins

KW - Mice

KW - Multigene Family

U2 - 10.1128/MCB.00272-08

DO - 10.1128/MCB.00272-08

M3 - SCORING: Journal article

C2 - 18505827

VL - 28

SP - 4688

EP - 4696

JO - MOL CELL BIOL

JF - MOL CELL BIOL

SN - 0270-7306

IS - 15

ER -