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Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor cells

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Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor cells. / Tiede, Stephan; Koop, Norbert; Kloepper, Jennifer E; Fässler, Reinhard; Paus, Ralf.

In: Stem cells (Dayton, Ohio), Vol. 27, No. 11, 11.2009, p. 2793-803.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Tiede, S, Koop, N, Kloepper, JE, Fässler, R & Paus, R 2009, 'Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor cells', Stem cells (Dayton, Ohio), vol. 27, no. 11, pp. 2793-803. https://doi.org/10.1002/stem.213

APA

Tiede, S., Koop, N., Kloepper, J. E., Fässler, R., & Paus, R. (2009). Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor cells. Stem cells (Dayton, Ohio), 27(11), 2793-803. https://doi.org/10.1002/stem.213

Vancouver

Tiede S, Koop N, Kloepper JE, Fässler R, Paus R. Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor cells. Stem cells (Dayton, Ohio). 2009 Nov;27(11):2793-803. https://doi.org/10.1002/stem.213

Bibtex

@article{f16fb43a2e1e45b2bf4fbaccf990f7ae,
title = "Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor cells",
abstract = "In this article we show that cloning of the human K15 promoter before a green fluorescence protein (GFP)/geneticin-resistance cassette and transfection of microdissected, organ-cultured adult human scalp hair follicles generates specific K15 promoter-driven GFP expression in their stem cell-rich bulge region. K15-GFP+ cells can be visualized in situ by GFP fluorescence and 2-photon laser scanning microscopy. Vital K15-GFP+ progenitor cells can then be selected by using the criteria of their green fluorescence, adhesion to collagen type IV and fibronectin, and geneticin resistance. Propagated K15-GFP+ cells express epithelial progenitor markers, show the expected differential gene expression profile of human bulge epithelium, and form holoclones. This application of nonretroviral, K15 promoter-driven, GFP labeling to adult human hair follicles facilitates the characterization and manipulation of human epithelial stem cells, both in situ and in vitro, and should be transferable to other complex human tissues.",
keywords = "Apoptosis, Cell Proliferation, Cell Survival, Cells, Cultured, Epithelial Cells, Fluorescent Antibody Technique, Green Fluorescent Proteins, Hair Follicle, Humans, Immunohistochemistry, Keratin-15, Oligonucleotide Array Sequence Analysis, Polymerase Chain Reaction, Promoter Regions, Genetic, Stem Cells, Journal Article, Research Support, Non-U.S. Gov't",
author = "Stephan Tiede and Norbert Koop and Kloepper, {Jennifer E} and Reinhard F{\"a}ssler and Ralf Paus",
year = "2009",
month = nov,
doi = "10.1002/stem.213",
language = "English",
volume = "27",
pages = "2793--803",
journal = "STEM CELLS",
issn = "1066-5099",
publisher = "ALPHAMED PRESS",
number = "11",

}

RIS

TY - JOUR

T1 - Nonviral in situ green fluorescent protein labeling and culture of primary, adult human hair follicle epithelial progenitor cells

AU - Tiede, Stephan

AU - Koop, Norbert

AU - Kloepper, Jennifer E

AU - Fässler, Reinhard

AU - Paus, Ralf

PY - 2009/11

Y1 - 2009/11

N2 - In this article we show that cloning of the human K15 promoter before a green fluorescence protein (GFP)/geneticin-resistance cassette and transfection of microdissected, organ-cultured adult human scalp hair follicles generates specific K15 promoter-driven GFP expression in their stem cell-rich bulge region. K15-GFP+ cells can be visualized in situ by GFP fluorescence and 2-photon laser scanning microscopy. Vital K15-GFP+ progenitor cells can then be selected by using the criteria of their green fluorescence, adhesion to collagen type IV and fibronectin, and geneticin resistance. Propagated K15-GFP+ cells express epithelial progenitor markers, show the expected differential gene expression profile of human bulge epithelium, and form holoclones. This application of nonretroviral, K15 promoter-driven, GFP labeling to adult human hair follicles facilitates the characterization and manipulation of human epithelial stem cells, both in situ and in vitro, and should be transferable to other complex human tissues.

AB - In this article we show that cloning of the human K15 promoter before a green fluorescence protein (GFP)/geneticin-resistance cassette and transfection of microdissected, organ-cultured adult human scalp hair follicles generates specific K15 promoter-driven GFP expression in their stem cell-rich bulge region. K15-GFP+ cells can be visualized in situ by GFP fluorescence and 2-photon laser scanning microscopy. Vital K15-GFP+ progenitor cells can then be selected by using the criteria of their green fluorescence, adhesion to collagen type IV and fibronectin, and geneticin resistance. Propagated K15-GFP+ cells express epithelial progenitor markers, show the expected differential gene expression profile of human bulge epithelium, and form holoclones. This application of nonretroviral, K15 promoter-driven, GFP labeling to adult human hair follicles facilitates the characterization and manipulation of human epithelial stem cells, both in situ and in vitro, and should be transferable to other complex human tissues.

KW - Apoptosis

KW - Cell Proliferation

KW - Cell Survival

KW - Cells, Cultured

KW - Epithelial Cells

KW - Fluorescent Antibody Technique

KW - Green Fluorescent Proteins

KW - Hair Follicle

KW - Humans

KW - Immunohistochemistry

KW - Keratin-15

KW - Oligonucleotide Array Sequence Analysis

KW - Polymerase Chain Reaction

KW - Promoter Regions, Genetic

KW - Stem Cells

KW - Journal Article

KW - Research Support, Non-U.S. Gov't

U2 - 10.1002/stem.213

DO - 10.1002/stem.213

M3 - SCORING: Journal article

C2 - 19750535

VL - 27

SP - 2793

EP - 2803

JO - STEM CELLS

JF - STEM CELLS

SN - 1066-5099

IS - 11

ER -