NK-1 receptor is involved in the decreased movement in a rat chronic acid reflux oesophagitis model
Standard
NK-1 receptor is involved in the decreased movement in a rat chronic acid reflux oesophagitis model. / Oshima, T; Koseki, J; Sakurai, J; Watari, J; Matsumoto, T; Miwa, H.
In: NEUROGASTROENT MOTIL, Vol. 22, No. 5, 05.2010, p. 579-84, e125.Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review
Harvard
APA
Vancouver
Bibtex
}
RIS
TY - JOUR
T1 - NK-1 receptor is involved in the decreased movement in a rat chronic acid reflux oesophagitis model
AU - Oshima, T
AU - Koseki, J
AU - Sakurai, J
AU - Watari, J
AU - Matsumoto, T
AU - Miwa, H
PY - 2010/5
Y1 - 2010/5
N2 - BACKGROUND: We previously reported that rats with reflux oesophagitis (RE) show a decrease in voluntary movement, which could be used as a measure of chronic visceral symptoms. However, what mediates these symptoms is still unknown, and pain-related neuropeptides or their receptors in oesophageal mucosa are possibly related to the symptom generation of oesophagitis. In the present study, we investigated the role of NK-1 receptor (NK-1R) as a mediator of oesophagitis symptoms.METHODS: Chronic RE was surgically induced using rats. The degree or severity of oesophageal symptoms was evaluated by assessing voluntary movement, which was monitored using an infrared sensor system. The NK-1R antagonist, L-732,138, was administered and changes in voluntary movement were assessed. Ten days after surgery, the rats were killed to examine the oesophagus. NK-1R and tachykinin-1 mRNA were detected by real-time RT-PCR. NK-1R protein expression was examined by Western blotting.KEY RESULTS: Voluntary movement of the oesophagitis model rats was significantly lower than that of the sham-operated rats on day 10. The size of oesophageal mucosal erosion did not correlate with the amount of voluntary movement. The amount of NK-1R protein and mRNA in the oesophageal tissue was significantly higher at both the erosion and non-erosion sites. The amount of tachykinin-1 mRNA in oesophageal tissue at the non-erosion sites was significantly higher in oesophagitis rats. The voluntary movement of oesophagitis rats was significantly increased by the administration of L-732,138.CONCLUSIONS & INFERENCES: The NK-1R and related neuropeptides are possibly involved in the decrease in voluntary movement of RE.
AB - BACKGROUND: We previously reported that rats with reflux oesophagitis (RE) show a decrease in voluntary movement, which could be used as a measure of chronic visceral symptoms. However, what mediates these symptoms is still unknown, and pain-related neuropeptides or their receptors in oesophageal mucosa are possibly related to the symptom generation of oesophagitis. In the present study, we investigated the role of NK-1 receptor (NK-1R) as a mediator of oesophagitis symptoms.METHODS: Chronic RE was surgically induced using rats. The degree or severity of oesophageal symptoms was evaluated by assessing voluntary movement, which was monitored using an infrared sensor system. The NK-1R antagonist, L-732,138, was administered and changes in voluntary movement were assessed. Ten days after surgery, the rats were killed to examine the oesophagus. NK-1R and tachykinin-1 mRNA were detected by real-time RT-PCR. NK-1R protein expression was examined by Western blotting.KEY RESULTS: Voluntary movement of the oesophagitis model rats was significantly lower than that of the sham-operated rats on day 10. The size of oesophageal mucosal erosion did not correlate with the amount of voluntary movement. The amount of NK-1R protein and mRNA in the oesophageal tissue was significantly higher at both the erosion and non-erosion sites. The amount of tachykinin-1 mRNA in oesophageal tissue at the non-erosion sites was significantly higher in oesophagitis rats. The voluntary movement of oesophagitis rats was significantly increased by the administration of L-732,138.CONCLUSIONS & INFERENCES: The NK-1R and related neuropeptides are possibly involved in the decrease in voluntary movement of RE.
KW - Animals
KW - Blotting, Western
KW - Disease Models, Animal
KW - Esophagitis, Peptic
KW - Esophagus
KW - Male
KW - Motor Activity
KW - Movement
KW - Neurokinin-1 Receptor Antagonists
KW - RNA, Messenger
KW - Rats
KW - Rats, Wistar
KW - Receptors, Neurokinin-1
KW - Reverse Transcriptase Polymerase Chain Reaction
KW - Tachykinins
KW - Tryptophan
KW - Journal Article
U2 - 10.1111/j.1365-2982.2009.01450.x
DO - 10.1111/j.1365-2982.2009.01450.x
M3 - SCORING: Journal article
C2 - 20059697
VL - 22
SP - 579-84, e125
JO - NEUROGASTROENT MOTIL
JF - NEUROGASTROENT MOTIL
SN - 1350-1925
IS - 5
ER -