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Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease

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Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease. / Schultheiß, Christoph; Paschold, Lisa; Simnica, Donjete; Mohme, Malte; Willscher, Edith; von Wenserski, Lisa; Scholz, Rebekka; Wieters, Imke; Dahlke, Christine; Tolosa, Eva; Sedding, Daniel G; Ciesek, Sandra; Addo, Marylyn; Binder, Mascha.

In: IMMUNITY, Vol. 53, No. 2, 18.08.2020, p. 442-455.e4.

Research output: SCORING: Contribution to journal › SCORING: Journal article › Research › peer-review

Harvard

Schultheiß, C, Paschold, L, Simnica, D, Mohme, M, Willscher, E, von Wenserski, L, Scholz, R, Wieters, I, Dahlke, C, Tolosa, E, Sedding, DG, Ciesek, S, Addo, M & Binder, M 2020, 'Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease', IMMUNITY, vol. 53, no. 2, pp. 442-455.e4. https://doi.org/10.1016/j.immuni.2020.06.024

APA

Schultheiß, C., Paschold, L., Simnica, D., Mohme, M., Willscher, E., von Wenserski, L., Scholz, R., Wieters, I., Dahlke, C., Tolosa, E., Sedding, D. G., Ciesek, S., Addo, M., & Binder, M. (2020). Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease. IMMUNITY, 53(2), 442-455.e4. https://doi.org/10.1016/j.immuni.2020.06.024

Vancouver

Schultheiß C, Paschold L, Simnica D, Mohme M, Willscher E, von Wenserski L et al. Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease. IMMUNITY. 2020 Aug 18;53(2):442-455.e4. https://doi.org/10.1016/j.immuni.2020.06.024

Bibtex

@article{e822e782721140d08b909ef543a2da37,
title = "Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease",
abstract = "We profiled adaptive immunity in COVID-19 patients with active infection or after recovery and created a repository of currently >14 million B and T cell receptor (BCR and TCR) sequences from the blood of these patients. The B cell response showed converging IGHV3-driven BCR clusters closely associated with SARS-CoV-2 antibodies. Clonality and skewing of TCR repertoires were associated with interferon type I and III responses, early CD4+ and CD8+ T cell activation, and counterregulation by the co-receptors BTLA, Tim-3, PD-1, TIGIT, and CD73. Tfh, Th17-like, and nonconventional (but not classical antiviral) Th1 cell polarizations were induced. SARS-CoV-2-specific T cell responses were driven by TCR clusters shared between patients with a characteristic trajectory of clonotypes and traceability over the disease course. Our data provide fundamental insight into adaptive immunity to SARS-CoV-2 with the actively updated repository providing a resource for the scientific community urgently needed to inform therapeutic concepts and vaccine development.",
author = "Christoph Schulthei{\ss} and Lisa Paschold and Donjete Simnica and Malte Mohme and Edith Willscher and {von Wenserski}, Lisa and Rebekka Scholz and Imke Wieters and Christine Dahlke and Eva Tolosa and Sedding, {Daniel G} and Sandra Ciesek and Marylyn Addo and Mascha Binder",
note = "Copyright {\textcopyright} 2020 Elsevier Inc. All rights reserved.",
year = "2020",
month = aug,
day = "18",
doi = "10.1016/j.immuni.2020.06.024",
language = "English",
volume = "53",
pages = "442--455.e4",
journal = "IMMUNITY",
issn = "1074-7613",
publisher = "Cell Press",
number = "2",

}

RIS

TY - JOUR

T1 - Next-Generation Sequencing of T and B Cell Receptor Repertoires from COVID-19 Patients Showed Signatures Associated with Severity of Disease

AU - Schultheiß, Christoph

AU - Paschold, Lisa

AU - Simnica, Donjete

AU - Mohme, Malte

AU - Willscher, Edith

AU - von Wenserski, Lisa

AU - Scholz, Rebekka

AU - Wieters, Imke

AU - Dahlke, Christine

AU - Tolosa, Eva

AU - Sedding, Daniel G

AU - Ciesek, Sandra

AU - Addo, Marylyn

AU - Binder, Mascha

N1 - Copyright © 2020 Elsevier Inc. All rights reserved.

PY - 2020/8/18

Y1 - 2020/8/18

N2 - We profiled adaptive immunity in COVID-19 patients with active infection or after recovery and created a repository of currently >14 million B and T cell receptor (BCR and TCR) sequences from the blood of these patients. The B cell response showed converging IGHV3-driven BCR clusters closely associated with SARS-CoV-2 antibodies. Clonality and skewing of TCR repertoires were associated with interferon type I and III responses, early CD4+ and CD8+ T cell activation, and counterregulation by the co-receptors BTLA, Tim-3, PD-1, TIGIT, and CD73. Tfh, Th17-like, and nonconventional (but not classical antiviral) Th1 cell polarizations were induced. SARS-CoV-2-specific T cell responses were driven by TCR clusters shared between patients with a characteristic trajectory of clonotypes and traceability over the disease course. Our data provide fundamental insight into adaptive immunity to SARS-CoV-2 with the actively updated repository providing a resource for the scientific community urgently needed to inform therapeutic concepts and vaccine development.

AB - We profiled adaptive immunity in COVID-19 patients with active infection or after recovery and created a repository of currently >14 million B and T cell receptor (BCR and TCR) sequences from the blood of these patients. The B cell response showed converging IGHV3-driven BCR clusters closely associated with SARS-CoV-2 antibodies. Clonality and skewing of TCR repertoires were associated with interferon type I and III responses, early CD4+ and CD8+ T cell activation, and counterregulation by the co-receptors BTLA, Tim-3, PD-1, TIGIT, and CD73. Tfh, Th17-like, and nonconventional (but not classical antiviral) Th1 cell polarizations were induced. SARS-CoV-2-specific T cell responses were driven by TCR clusters shared between patients with a characteristic trajectory of clonotypes and traceability over the disease course. Our data provide fundamental insight into adaptive immunity to SARS-CoV-2 with the actively updated repository providing a resource for the scientific community urgently needed to inform therapeutic concepts and vaccine development.

U2 - 10.1016/j.immuni.2020.06.024

DO - 10.1016/j.immuni.2020.06.024

M3 - SCORING: Journal article

C2 - 32668194

VL - 53

SP - 442-455.e4

JO - IMMUNITY

JF - IMMUNITY

SN - 1074-7613

IS - 2

ER -